Gene description for Trf
Gene name transferrin
Gene symbol Trf
Other names/aliases AI266983
Cd176
HP
Tf
Tfn
hpx
Species Mus musculus
 Database cross references - Trf
ExoCarta ExoCarta_22041
Vesiclepedia VP_22041
Entrez Gene 22041
UniProt Q921I1  
 Trf identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Trf
Molecular Function
    ferric iron binding GO:0008199 ISO
    ferric iron transmembrane transporter activity GO:0015091 ISO
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of oligodendrocyte progenitor proliferation GO:0070447 ISO
    ion transport GO:0006811 IEA
    positive regulation of phosphorylation GO:0042327 IDA
    transport GO:0006810 IEA
    osteoclast differentiation GO:0030316 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    positive regulation of bone resorption GO:0045780 IDA
    iron ion homeostasis GO:0055072 IEA
    ERK1 and ERK2 cascade GO:0070371 IDA
    iron ion transport GO:0006826 IMP
    ferric iron transport GO:0015682 ISO
    iron ion transmembrane transport GO:0034755 ISO
    positive regulation of myelination GO:0031643 ISO
    positive regulation of cell motility GO:2000147 IDA
    activation of JUN kinase activity GO:0007257 IDA
    actin filament organization GO:0007015 IDA
    SMAD protein signal transduction GO:0060395 IDA
    cellular iron ion homeostasis GO:0006879 IEA
Subcellular Localization
    early endosome GO:0005769 ISO
    coated pit GO:0005905 ISO
    extracellular exosome GO:0070062 ISO
    dense body GO:0097433 ISO
    extracellular space GO:0005615 ISO
    late endosome GO:0005770 ISO
    basal plasma membrane GO:0009925 ISO
    apical plasma membrane GO:0016324 ISO
    vesicle coat GO:0030120 ISO
    endocytic vesicle GO:0030139 ISO
    cell surface GO:0009986 ISO
    vesicle GO:0031982 ISO
    basement membrane GO:0005604 ISO
    blood microparticle GO:0072562 ISO
    membrane GO:0016020 IMP
    endosome GO:0005768 IDA
    cytoplasmic membrane-bounded vesicle GO:0016023 ISO
    basal part of cell GO:0045178 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    extracellular region GO:0005576 IEA
    recycling endosome GO:0055037 ISO
 Experiment description of studies that identified Trf in exosomes
1
Experiment ID 26
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 303
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd63|Cd81|Cd9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG.
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 304
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd63|Cd81|Cd9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG.
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
5
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Trf
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Trf is involved
PathwayEvidenceSource
Iron uptake and transport IEA Reactome
Platelet degranulation IEA Reactome
Transferrin endocytosis and recycling IEA Reactome





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