Gene description for Capzb
Gene name capping protein (actin filament) muscle Z-line, beta
Gene symbol Capzb
Other names/aliases 1700120C01Rik
AI325129
CPB1
CPB2
CPbeat2
CPbeta1
CPbeta2
Cappb1
Species Mus musculus
 Database cross references - Capzb
ExoCarta ExoCarta_12345
Vesiclepedia VP_12345
Entrez Gene 12345
UniProt P47757  
 Capzb identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Capzb
Molecular Function
    beta-tubulin binding GO:0048487 IPI
    protein binding GO:0005515 IPI
    actin binding GO:0003779 IEA
    actin filament binding GO:0051015 IDA
Biological Process
    regulation of protein kinase C signaling GO:0090036 IMP
    cytoskeleton organization GO:0007010 ISO
    negative regulation of filopodium assembly GO:0051490 IBA
    actin filament capping GO:0051693 IEA
    regulation of lamellipodium assembly GO:0010591 IBA
    muscle fiber development GO:0048747 IMP
    cell morphogenesis GO:0000902 IBA
    cell projection organization GO:0030030 IMP
    actin cytoskeleton organization GO:0030036 IEA
    barbed-end actin filament capping GO:0051016 IDA
    negative regulation of microtubule polymerization GO:0031115 IDA
    neuron projection development GO:0031175 IMP
    lamellipodium assembly GO:0030032 IMP
    regulation of cell morphogenesis GO:0022604 ISO
Subcellular Localization
    F-actin capping protein complex GO:0008290 IPI
    WASH complex GO:0071203 ISO
    cytoskeleton GO:0005856 IEA
    Z disc GO:0030018 ISA
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IDA
    dendritic spine GO:0043197 ISO
    neuronal cell body GO:0043025 ISO
    cytoplasm GO:0005737 IEA
    intercalated disc GO:0014704 ISA
    acrosomal vesicle GO:0001669 IDA
    cortical cytoskeleton GO:0030863 IDA
    lamellipodium GO:0030027 IDA
    brush border GO:0005903 IDA
    actin filament GO:0005884 IBA
 Experiment description of studies that identified Capzb in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Capzb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Capzb is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production IEA Reactome





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