Gene description for Myo5a
Gene name myosin VA
Gene symbol Myo5a
Other names/aliases 9630007J19Rik
AI413174
AI661011
Dbv
MVa
Myo5
MyoVA
Sev-1
d
d-120J
flail
flr
Species Mus musculus
 Database cross references - Myo5a
ExoCarta ExoCarta_17918
Vesiclepedia VP_17918
Entrez Gene 17918
UniProt Q99104  
 Myo5a identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myo5a
Molecular Function
    microfilament motor activity GO:0000146 ISO
    calcium ion binding GO:0005509 IDA
    protein heterodimerization activity GO:0046982 ISO
    calcium-dependent protein binding GO:0048306 ISO
    ATP-dependent protein binding GO:0043008 ISO
    protein dimerization activity GO:0046983 ISO
    calmodulin binding GO:0005516 IDA
    SNARE binding GO:0000149 ISO
    ATP binding GO:0005524 ISO
    nucleotide binding GO:0000166 IEA
    syntaxin-1 binding GO:0017075 ISO
    actin binding GO:0003779 IDA
    poly(A) RNA binding GO:0044822 ISO
    protein binding GO:0005515 IPI
    Rab GTPase binding GO:0017137 ISO
    motor activity GO:0003774 ISO
Biological Process
    endoplasmic reticulum localization GO:0051643 IMP
    locomotion involved in locomotory behavior GO:0031987 IMP
    transport GO:0006810 IEA
    odontogenesis GO:0042476 IDA
    vesicle transport along actin filament GO:0030050 TAS
    myelination GO:0042552 IMP
    melanosome transport GO:0032402 IMP
    regulation of exocytosis GO:0017157 ISO
    visual perception GO:0007601 IMP
    protein transport GO:0015031 IEA
    cellular response to insulin stimulus GO:0032869 IMP
    melanocyte differentiation GO:0030318 IMP
    long-chain fatty acid biosynthetic process GO:0042759 IMP
    melanin biosynthetic process GO:0042438 IMP
    melanin metabolic process GO:0006582 IMP
    metabolic process GO:0008152 ISO
    vesicle-mediated transport GO:0016192 IMP
    insulin secretion GO:0030073 IMP
    actin filament-based movement GO:0030048 ISO
    protein localization to plasma membrane GO:0072659 IMP
    anagen GO:0042640 IMP
    developmental pigmentation GO:0048066 IMP
    post-Golgi vesicle-mediated transport GO:0006892 ISO
    melanosome localization GO:0032400 IMP
    synapse organization GO:0050808 IMP
    secretory granule localization GO:0032252 ISO
    pigmentation GO:0043473 ISA
    neuromuscular process controlling balance GO:0050885 IMP
    regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity GO:0031585 IMP
    exocytosis GO:0006887 IMP
    synaptic transmission GO:0007268 IMP
Subcellular Localization
    neuron projection GO:0043005 ISO
    photoreceptor outer segment GO:0001750 IDA
    melanosome GO:0042470 IDA
    peroxisome GO:0005777 ISO
    lysosome GO:0005764 ISO
    ruffle GO:0001726 ISO
    cell GO:0005623 IMP
    axon GO:0030424 ISO
    microtubule plus-end GO:0035371 IDA
    extracellular exosome GO:0070062 ISO
    myosin complex GO:0016459 IDA
    actomyosin, myosin complex part GO:0042642 ISO
    neuronal cell body GO:0043025 ISO
    intermediate filament GO:0005882 IDA
    synaptic vesicle GO:0008021 ISO
    membrane GO:0016020 ISO
    Golgi apparatus GO:0005794 IDA
    early endosome GO:0005769 ISO
    cytosol GO:0005829 ISO
    late endosome GO:0005770 ISO
    recycling endosome GO:0055037 ISO
    cytoplasm GO:0005737 ISO
    vesicle GO:0031982 ISO
    endoplasmic reticulum GO:0005783 ISO
    actomyosin GO:0042641 IDA
    secretory granule GO:0030141 ISO
    insulin-responsive compartment GO:0032593 IDA
    filopodium tip GO:0032433 ISO
    actin filament GO:0005884 ISO
 Experiment description of studies that identified Myo5a in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myo5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Myo5a is involved
PathwayEvidenceSource
Insulin processing IEA Reactome
Translocation of GLUT4 to the plasma membrane IEA Reactome





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