Gene description for Myo5a
Gene name myosin VA
Gene symbol Myo5a
Other names/aliases 9630007J19Rik
AI413174
AI661011
Dbv
MVa
Myo5
MyoVA
Sev-1
d
d-120J
flail
flr
Species Mus musculus
 Database cross references - Myo5a
ExoCarta ExoCarta_17918
Vesiclepedia VP_17918
Entrez Gene 17918
UniProt Q99104  
 Myo5a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myo5a
Molecular Function
    microfilament motor activity GO:0000146 IBA
    microfilament motor activity GO:0000146 IDA
    microfilament motor activity GO:0000146 ISO
    microfilament motor activity GO:0000146 TAS
    SNARE binding GO:0000149 ISO
    cytoskeletal motor activity GO:0003774 IDA
    cytoskeletal motor activity GO:0003774 ISO
    actin binding GO:0003779 IDA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    syntaxin-1 binding GO:0017075 ISO
    protein kinase binding GO:0019901 ISO
    small GTPase binding GO:0031267 IPI
    small GTPase binding GO:0031267 ISO
    identical protein binding GO:0042802 IPI
    ATP-dependent protein binding GO:0043008 ISO
    protein-containing complex binding GO:0044877 ISO
    calcium-dependent protein binding GO:0048306 ISO
    actin filament binding GO:0051015 IBA
    disordered domain specific binding GO:0097718 IPI
Biological Process
    melanin metabolic process GO:0006582 IMP
    exocytosis GO:0006887 IMP
    post-Golgi vesicle-mediated transport GO:0006892 ISO
    endocytosis GO:0006897 IBA
    actin filament organization GO:0007015 IBA
    chemical synaptic transmission GO:0007268 IMP
    visual perception GO:0007601 IMP
    vesicle-mediated transport GO:0016192 IMP
    macroautophagy GO:0016236 ISO
    regulation of exocytosis GO:0017157 ISO
    actin filament-based movement GO:0030048 IDA
    actin filament-based movement GO:0030048 ISO
    vesicle transport along actin filament GO:0030050 ISO
    vesicle transport along actin filament GO:0030050 TAS
    insulin secretion GO:0030073 IMP
    melanocyte differentiation GO:0030318 IMP
    regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity GO:0031585 IMP
    locomotion involved in locomotory behavior GO:0031987 IMP
    secretory granule localization GO:0032252 IMP
    secretory granule localization GO:0032252 ISO
    melanosome localization GO:0032400 IMP
    melanosome transport GO:0032402 IDA
    melanosome transport GO:0032402 IMP
    cellular response to insulin stimulus GO:0032869 IMP
    negative regulation of dopamine secretion GO:0033602 ISO
    dopamine metabolic process GO:0042417 ISO
    melanin biosynthetic process GO:0042438 IMP
    odontogenesis GO:0042476 IDA
    myelination GO:0042552 IMP
    anagen GO:0042640 IMP
    long-chain fatty acid biosynthetic process GO:0042759 IMP
    pigmentation GO:0043473 IMP
    pigmentation GO:0043473 ISO
    developmental pigmentation GO:0048066 IMP
    hair follicle maturation GO:0048820 IMP
    synapse organization GO:0050808 IMP
    neuromuscular process controlling balance GO:0050885 IMP
    endoplasmic reticulum localization GO:0051643 IMP
    negative regulation of synaptic transmission, glutamatergic GO:0051967 ISO
    protein-containing complex assembly GO:0065003 ISO
    protein localization to plasma membrane GO:0072659 IMP
    establishment of endoplasmic reticulum localization to postsynapse GO:0099089 IDA
    establishment of endoplasmic reticulum localization to postsynapse GO:0099089 IMP
    regulation of postsynaptic cytosolic calcium ion concentration GO:0099566 IDA
    regulation of postsynaptic cytosolic calcium ion concentration GO:0099566 IMP
    axo-dendritic protein transport GO:0099640 ISO
    reactive gliosis GO:0150103 ISO
    positive regulation of cellular response to insulin stimulus GO:1900078 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 ISO
Subcellular Localization
    ruffle GO:0001726 ISO
    photoreceptor outer segment GO:0001750 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    lysosome GO:0005764 ISO
    early endosome GO:0005769 ISO
    late endosome GO:0005770 ISO
    peroxisome GO:0005777 ISO
    endoplasmic reticulum GO:0005783 ISO
    smooth endoplasmic reticulum GO:0005790 IDA
    smooth endoplasmic reticulum GO:0005790 IMP
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 IDA
    actin filament GO:0005884 ISO
    synaptic vesicle GO:0008021 ISO
    actin cytoskeleton GO:0015629 IBA
    membrane GO:0016020 IBA
    membrane GO:0016020 ISO
    myosin complex GO:0016459 IDA
    unconventional myosin complex GO:0016461 IMP
    secretory granule GO:0030141 IDA
    secretory granule GO:0030141 ISO
    axon GO:0030424 ISO
    dendrite GO:0030425 ISO
    vesicle GO:0031982 ISO
    filopodium tip GO:0032433 ISO
    insulin-responsive compartment GO:0032593 IDA
    melanosome GO:0042470 IDA
    melanosome GO:0042470 ISO
    actomyosin GO:0042641 IDA
    actomyosin, myosin complex part GO:0042642 ISO
    neuronal cell body GO:0043025 IDA
    neuronal cell body GO:0043025 ISO
    recycling endosome GO:0055037 ISO
    recycling endosome GO:0055037 ISO
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IMP
    postsynapse GO:0098794 ISO
    postsynaptic actin cytoskeleton GO:0098871 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Myo5a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myo5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
2 SNCA 6622
Affinity Capture-MS Homo sapiens
3 Cyfip2 76884
Affinity Capture-MS Mus musculus
4 Tnik  
Affinity Capture-MS Mus musculus
5 Kalrn  
Affinity Capture-MS Mus musculus
6 DCP1B  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
7 Elf5  
Affinity Capture-MS Mus musculus
8 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
12 Pex5l  
Affinity Capture-MS Mus musculus
13 Ywhaz 22631
Affinity Capture-MS Mus musculus
14 VASP 7408
Affinity Capture-MS Homo sapiens
15 Nphp4  
Affinity Capture-MS Mus musculus
16 Cyfip1 20430
Affinity Capture-MS Mus musculus
17 Myh11  
Co-fractionation Mus musculus
18 Atg5  
Affinity Capture-MS Mus musculus
19 DCP1A 55802
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
20 Tmf1  
Co-fractionation Mus musculus
21 Fancd2  
Affinity Capture-MS Mus musculus
22 Myh14 71960
Co-fractionation Mus musculus
23 Mlph  
Co-fractionation Mus musculus
24 Agap2  
Affinity Capture-MS Mus musculus
25 Myh8  
Co-fractionation Mus musculus
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