Gene description for Myh14
Gene name myosin, heavy polypeptide 14
Gene symbol Myh14
Other names/aliases 2400004E04Rik
II-C
NHMCII
NMHC II-C
Species Mus musculus
 Database cross references - Myh14
ExoCarta ExoCarta_71960
Vesiclepedia VP_71960
Entrez Gene 71960
UniProt Q6URW6  
 Myh14 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myh14
Molecular Function
    microfilament motor activity GO:0000146 IDA
    microfilament motor activity GO:0000146 ISO
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IDA
    actin filament binding GO:0051015 IDA
    actin filament binding GO:0051015 ISO
    molecular adaptor activity GO:0060090 EXP
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    skeletal muscle contraction GO:0003009 IEA
    skeletal muscle contraction GO:0003009 ISO
    mitochondrion organization GO:0007005 IEA
    mitochondrion organization GO:0007005 ISO
    skeletal muscle tissue development GO:0007519 IEA
    skeletal muscle tissue development GO:0007519 ISO
    sensory perception of sound GO:0007605 IEA
    sensory perception of sound GO:0007605 ISO
    regulation of cell shape GO:0008360 IBA
    regulation of cell shape GO:0008360 IEA
    neuronal action potential GO:0019228 IEA
    neuronal action potential GO:0019228 ISO
    actin filament-based movement GO:0030048 IDA
    actomyosin structure organization GO:0031032 IBA
    actomyosin structure organization GO:0031032 IEA
    actomyosin structure organization GO:0031032 ISO
    vocalization behavior GO:0071625 IEA
    vocalization behavior GO:0071625 ISO
Subcellular Localization
    stress fiber GO:0001725 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    brush border GO:0005903 IDA
    myosin complex GO:0016459 IDA
    myosin II complex GO:0016460 IBA
    myosin II complex GO:0016460 IEA
    myosin II complex GO:0016460 ISO
    axon GO:0030424 IDA
    growth cone GO:0030426 IDA
    myosin filament GO:0032982 IBA
    actomyosin GO:0042641 ISO
    myelin sheath GO:0043209 HDA
    myosin II filament GO:0097513 IEA
    myosin II filament GO:0097513 ISO
 Experiment description of studies that identified Myh14 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myh14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Myh11  
Co-fractionation Mus musculus
2 Zbtb7b  
Affinity Capture-MS Mus musculus
3 Fancd2  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Myo5a 17918
Co-fractionation Mus musculus
View the network image/svg+xml



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