Gene description for Aak1
Gene name AP2 associated kinase 1
Gene symbol Aak1
Other names/aliases 5530400K14Rik
9630042K20
AU067724
AU067726
BC028270
C79663
D6Ertd245e
R75501
mKIAA1048
Species Mus musculus
 Database cross references - Aak1
ExoCarta ExoCarta_269774
Vesiclepedia VP_269774
Entrez Gene 269774
UniProt Q3UHJ0  
 Aak1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Aak1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    Notch binding GO:0005112 ISO
    Notch binding GO:0005112 ISS
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    AP-2 adaptor complex binding GO:0035612 IBA
    AP-2 adaptor complex binding GO:0035612 ISO
    AP-2 adaptor complex binding GO:0035612 ISS
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    regulation of protein localization GO:0032880 ISO
    regulation of protein localization GO:0032880 ISS
    positive regulation of Notch signaling pathway GO:0045747 ISO
    positive regulation of Notch signaling pathway GO:0045747 ISS
    protein stabilization GO:0050821 ISO
    protein stabilization GO:0050821 ISS
    presynaptic endocytosis GO:0140238 IDA
    presynaptic endocytosis GO:0140238 IMP
    presynaptic endocytosis GO:0140238 ISO
    regulation of clathrin-dependent endocytosis GO:2000369 IBA
    regulation of clathrin-dependent endocytosis GO:2000369 ISO
    regulation of clathrin-dependent endocytosis GO:2000369 ISS
Subcellular Localization
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    clathrin-coated pit GO:0005905 ISS
    clathrin-coated vesicle GO:0030136 ISS
    cell leading edge GO:0031252 ISO
    cell leading edge GO:0031252 ISS
    terminal bouton GO:0043195 ISO
    terminal bouton GO:0043195 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    calyx of Held GO:0044305 ISO
    clathrin complex GO:0071439 ISO
    presynapse GO:0098793 IBA
    presynapse GO:0098793 ISO
 Experiment description of studies that identified Aak1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Aak1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Syngap1  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Foxp3  
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Traf3  
Proximity Label-MS Mus musculus
7 Mapt  
Affinity Capture-MS Mus musculus
8 Atg5  
Affinity Capture-RNA Mus musculus
9 Cyfip1 20430
Affinity Capture-MS Mus musculus
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