Gene description for Aak1
Gene name AP2 associated kinase 1
Gene symbol Aak1
Other names/aliases 5530400K14Rik
9630042K20
AU067724
AU067726
BC028270
C79663
D6Ertd245e
R75501
mKIAA1048
Species Mus musculus
 Database cross references - Aak1
ExoCarta ExoCarta_269774
Vesiclepedia VP_269774
Entrez Gene 269774
UniProt Q3UHJ0  
 Aak1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Aak1
Molecular Function
    Notch binding GO:0005112 ISO
    protein serine/threonine kinase activity GO:0004674 ISO
    AP-2 adaptor complex binding GO:0035612 ISO
Biological Process
    protein stabilization GO:0050821 ISO
    protein autophosphorylation GO:0046777 ISO
    regulation of protein localization GO:0032880 ISO
    regulation of clathrin-mediated endocytosis GO:2000369 ISO
    protein phosphorylation GO:0006468 ISO
    positive regulation of Notch signaling pathway GO:0045747 ISO
Subcellular Localization
    terminal bouton GO:0043195 ISO
    cell leading edge GO:0031252 ISO
    clathrin complex GO:0071439 ISO
 Experiment description of studies that identified Aak1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Aak1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Aak1 is involved
No pathways found





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