Gene description for Arhgdia
Gene name Rho GDP dissociation inhibitor (GDI) alpha
Gene symbol Arhgdia
Other names/aliases 5330430M07Rik
Gdi-1
Rho-GDI
RhoDGI
RhoGDI-1
Species Mus musculus
 Database cross references - Arhgdia
ExoCarta ExoCarta_192662
Entrez Gene 192662
UniProt Q99PT1  
 Arhgdia identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
 Gene ontology annotations for Arhgdia
Molecular Function
    GTPase activator activity GO:0005096 IEA
    fatty acid binding GO:0005504 ISO
    protein kinase binding GO:0019901 ISO
    Hsp90 protein binding GO:0051879 ISO
    protein binding GO:0005515 IPI
    Rho GTPase binding GO:0017048 ISO
    Rho GDP-dissociation inhibitor activity GO:0005094 IDA
Biological Process
    negative regulation of cell migration GO:0030336 ISO
    regulation of protein localization GO:0032880 IDA
    regulation of cell shape GO:0008360 ISO
    cellular response to mechanical stimulus GO:0071260 ISO
    regulation of catalytic activity GO:0050790 IDA
    Rho protein signal transduction GO:0007266 IDA
    semaphorin-plexin signaling pathway GO:0071526 IMP
Subcellular Localization
    cytosol GO:0005829 ISO
    nucleus GO:0005634 IDA
    extracellular space GO:0005615 ISO
    immunological synapse GO:0001772 IDA
    cytoplasm GO:0005737 IEA
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Arhgdia in exosomes
1
Experiment ID 73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Arhgdia
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Arhgdia is involved
PathwayEvidenceSource
Axonal growth inhibition (RHOA activation) IEA Reactome
Axonal growth stimulation IEA Reactome
Rho GTPase cycle IEA Reactome





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