Gene description for TRPC6
Gene name transient receptor potential cation channel, subfamily C, member 6
Gene symbol TRPC6
Other names/aliases FSGS2
TRP6
Species Homo sapiens
 Database cross references - TRPC6
ExoCarta ExoCarta_7225
Entrez Gene 7225
HGNC 12338
MIM 603652
UniProt Q9Y210  
 TRPC6 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for TRPC6
Molecular Function
    protein binding GO:0005515 IPI
    inositol 1,4,5 trisphosphate binding GO:0070679 IDA
    store-operated calcium channel activity GO:0015279 IBA
Biological Process
    cytosolic calcium ion homeostasis GO:0051480 IBA
    cation transport GO:0006812 TAS
    positive regulation of calcium ion transport GO:0051928 IDA
    blood coagulation GO:0007596 TAS
    ion transmembrane transport GO:0034220 TAS
    manganese ion transport GO:0006828 IBA
    positive regulation of ion transmembrane transporter activity GO:0032414 IDA
    platelet activation GO:0030168 TAS
    axon guidance GO:0007411 TAS
    transmembrane transport GO:0055085 TAS
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
    calcium ion transmembrane transport GO:0070588 TAS
    aging GO:0007568 IEA
    cellular response to hypoxia GO:0071456 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    slit diaphragm GO:0036057 IEA
    membrane GO:0016020 IDA
    integral component of plasma membrane GO:0005887 IBA
 Experiment description of studies that identified TRPC6 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRPC6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITPR1  
Affinity Capture-Western Homo sapiens
2 TRPC5  
Affinity Capture-Western Homo sapiens
3 SRC 6714
Affinity Capture-Western Homo sapiens
4 TRPC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 TRPC6 7225
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
6 TRPC1  
Affinity Capture-Western Homo sapiens
7 TRPC3  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
8 FYN 2534
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 TRPC4  
Affinity Capture-Western Homo sapiens
10 ITPR3 3710
Reconstituted Complex Homo sapiens
11 TRPC7  
FRET Homo sapiens
View the network image/svg+xml



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