Gene description for TRPC6
Gene name transient receptor potential cation channel, subfamily C, member 6
Gene symbol TRPC6
Other names/aliases FSGS2
TRP6
Species Homo sapiens
 Database cross references - TRPC6
ExoCarta ExoCarta_7225
Vesiclepedia VP_7225
Entrez Gene 7225
HGNC 12338
MIM 603652
UniProt Q9Y210  
 TRPC6 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for TRPC6
Molecular Function
    monoatomic cation channel activity GO:0005261 IDA
    calcium channel activity GO:0005262 IDA
    calcium channel activity GO:0005262 IMP
    calcium channel activity GO:0005262 TAS
    protein binding GO:0005515 IPI
    store-operated calcium channel activity GO:0015279 IBA
    protein homodimerization activity GO:0042803 IDA
    inositol 1,4,5 trisphosphate binding GO:0070679 IBA
    inositol 1,4,5 trisphosphate binding GO:0070679 IDA
Biological Process
    monoatomic cation transport GO:0006812 TAS
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    single fertilization GO:0007338 IBA
    positive regulation of ion transmembrane transporter activity GO:0032414 IDA
    regulation of cytosolic calcium ion concentration GO:0051480 IBA
    positive regulation of calcium ion transport GO:0051928 IDA
    calcium ion transmembrane transport GO:0070588 IBA
    calcium ion transmembrane transport GO:0070588 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
    cation channel complex GO:0034703 IBA
    slit diaphragm GO:0036057 IEA
 Experiment description of studies that identified TRPC6 in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRPC6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITPR1 3708
Affinity Capture-Western Homo sapiens
2 TRPC5  
Affinity Capture-Western Homo sapiens
3 SRC 6714
Affinity Capture-Western Homo sapiens
4 TRPC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 RNF24  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 TRPC6 7225
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
7 TRPC1  
Affinity Capture-Western Homo sapiens
8 TRPC3  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
9 FYN 2534
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 TRPC4  
Affinity Capture-Western Homo sapiens
11 ITPR3 3710
Reconstituted Complex Homo sapiens
12 TRPC7  
FRET Homo sapiens
View the network image/svg+xml



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