Gene description for SH3GLB2
Gene name SH3-domain GRB2-like endophilin B2
Gene symbol SH3GLB2
Other names/aliases PP6569
PP9455
RRIG1
Species Homo sapiens
 Database cross references - SH3GLB2
ExoCarta ExoCarta_56904
Vesiclepedia VP_56904
Entrez Gene 56904
HGNC 10834
MIM 609288
UniProt Q9NR46  
 SH3GLB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SH3GLB2
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    cadherin binding GO:0045296 HDA
Biological Process
    membrane organization GO:0061024 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 IBA
 Experiment description of studies that identified SH3GLB2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SH3GLB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EFHC1  
Two-hybrid Homo sapiens
2 CNOT7 29883
Two-hybrid Homo sapiens
3 MAVS 57506
Proximity Label-MS Homo sapiens
4 OGT 8473
Reconstituted Complex Homo sapiens
5 PSMA3 5684
Affinity Capture-MS Homo sapiens
6 GMPPB 29925
Affinity Capture-MS Homo sapiens
7 SREK1 140890
Two-hybrid Homo sapiens
8 KRTAP10-8  
Two-hybrid Homo sapiens
9 UBA5 79876
Affinity Capture-MS Homo sapiens
10 NMI  
Two-hybrid Homo sapiens
11 RABEP2 79874
Affinity Capture-MS Homo sapiens
12 EXOSC1 51013
Two-hybrid Homo sapiens
13 TBC1D7  
Two-hybrid Homo sapiens
14 LZTFL1 54585
Co-fractionation Homo sapiens
15 GADD45G  
Two-hybrid Homo sapiens
16 CALU 813
Co-fractionation Homo sapiens
17 GIT2 9815
Two-hybrid Homo sapiens
18 AARSD1 80755
Co-fractionation Homo sapiens
19 UBR3  
Affinity Capture-MS Homo sapiens
20 FHL3 2275
Two-hybrid Homo sapiens
21 TEX11  
Two-hybrid Homo sapiens
22 KRTAP9-3  
Two-hybrid Homo sapiens
23 MAGEA1  
Two-hybrid Homo sapiens
24 TSG101 7251
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 CCDC158 339965
Two-hybrid Homo sapiens
26 FKBP4 2288
Affinity Capture-MS Homo sapiens
27 BLMH 642
Co-fractionation Homo sapiens
28 TGM2 7052
Co-fractionation Homo sapiens
29 GAA 2548
Two-hybrid Homo sapiens
30 PPP6R2 9701
Proximity Label-MS Homo sapiens
31 MORF4L1  
Two-hybrid Homo sapiens
32 RPUSD2  
Co-fractionation Homo sapiens
33 PA2G4 5036
Affinity Capture-MS Homo sapiens
34 SH3GL1 6455
Co-fractionation Homo sapiens
35 NXF1 10482
Two-hybrid Homo sapiens
36 APEX1 328
Affinity Capture-RNA Homo sapiens
37 EFTUD2 9343
Co-fractionation Homo sapiens
38 RNH1 6050
Co-fractionation Homo sapiens
39 PRMT5 10419
Co-fractionation Homo sapiens
40 KRTAP5-11  
Two-hybrid Homo sapiens
41 MBNL1 4154
Affinity Capture-MS Homo sapiens
42 KRTAP4-5  
Two-hybrid Homo sapiens
43 KDM1A 23028
Two-hybrid Homo sapiens
44 PGK1 5230
Affinity Capture-MS Homo sapiens
45 CDH1 999
Proximity Label-MS Homo sapiens
46 ZDHHC6  
Affinity Capture-MS Homo sapiens
47 BTBD1  
Affinity Capture-MS Homo sapiens
48 LSM3 27258
Two-hybrid Homo sapiens
49 WDYHV1  
Two-hybrid Homo sapiens
50 SH3GLB1 51100
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PITPNB 23760
Cross-Linking-MS (XL-MS) Homo sapiens
52 CRTAP 10491
Co-fractionation Homo sapiens
53 EXOSC5 56915
Two-hybrid Homo sapiens
54 GTF2E1 2960
Co-fractionation Homo sapiens
55 YES1 7525
Two-hybrid Homo sapiens
56 SSUH2  
Affinity Capture-MS Homo sapiens
57 SERBP1 26135
Affinity Capture-MS Homo sapiens
58 THG1L 54974
Co-fractionation Homo sapiens
59 TBCD 6904
Co-fractionation Homo sapiens
60 SH3KBP1 30011
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
61 STK4 6789
Proximity Label-MS Homo sapiens
62 CDKN2D  
Two-hybrid Homo sapiens
63 STX4 6810
Two-hybrid Homo sapiens
64 RHOA 387
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
65 BRDT  
Affinity Capture-MS Homo sapiens
66 PICALM 8301
Co-fractionation Homo sapiens
67 STIP1 10963
Co-fractionation Homo sapiens
68 DNM1L 10059
Proximity Label-MS Homo sapiens
69 KRTAP10-1  
Two-hybrid Homo sapiens
70 TPM3 7170
Co-fractionation Homo sapiens
71 ZNF302  
Affinity Capture-MS Homo sapiens
72 CCND3  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SH3GLB2 is involved
No pathways found





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