Gene description for NRD1
Gene name nardilysin (N-arginine dibasic convertase)
Gene symbol NRD1
Other names/aliases hNRD1
hNRD2
Species Homo sapiens
 Database cross references - NRD1
ExoCarta ExoCarta_4898
Vesiclepedia VP_4898
Entrez Gene 4898
HGNC 7995
MIM 602651
UniProt O43847  
 NRD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NRD1
Molecular Function
    metalloendopeptidase activity GO:0004222 NAS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    epidermal growth factor binding GO:0048408 TAS
Biological Process
    proteolysis GO:0006508 IEA
    positive regulation of myelination GO:0031643 ISS
    positive regulation of axonogenesis GO:0050772 ISS
    positive regulation of membrane protein ectodomain proteolysis GO:0051044 IDA
    regulation of endopeptidase activity GO:0052548 IDA
    negative regulation of cold-induced thermogenesis GO:0120163 ISS
Subcellular Localization
    mitochondrial matrix GO:0005759 IDA
    cytosol GO:0005829 TAS
    cell surface GO:0009986 TAS
    dendrite GO:0030425 IEA
 Experiment description of studies that identified NRD1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NRD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PRM2  
Affinity Capture-MS Homo sapiens
3 PPP3CA 5530
Co-fractionation Homo sapiens
4 KPNA3 3839
Co-fractionation Homo sapiens
5 Fus  
Affinity Capture-MS Mus musculus
6 Mtx2  
Affinity Capture-MS Mus musculus
7 TRUB2  
Affinity Capture-MS Homo sapiens
8 KLRK1  
Affinity Capture-MS Homo sapiens
9 CHCHD1  
Affinity Capture-MS Homo sapiens
10 ATM 472
Affinity Capture-MS Homo sapiens
11 KIF7 374654
Affinity Capture-MS Homo sapiens
12 SOX2  
Affinity Capture-MS Homo sapiens
13 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
14 C9orf78 51759
Affinity Capture-MS Homo sapiens
15 HDAC3 8841
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 NCOR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
17 METTL25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CHCHD2  
Affinity Capture-MS Homo sapiens
19 IQGAP1 8826
Affinity Capture-MS Homo sapiens
20 PDIA6 10130
Co-fractionation Homo sapiens
21 P3H1 64175
Co-fractionation Homo sapiens
22 Haus1  
Affinity Capture-MS Mus musculus
23 ERMAP 114625
Affinity Capture-MS Homo sapiens
24 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SEPT1 1731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TSN 7247
Co-fractionation Homo sapiens
27 UIMC1  
Affinity Capture-MS Homo sapiens
28 ACP6 51205
Affinity Capture-MS Homo sapiens
29 COBLL1 22837
Affinity Capture-MS Homo sapiens
30 XRCC4  
Co-fractionation Homo sapiens
31 NSUN2 54888
Co-fractionation Homo sapiens
32 ATP5A1 498
Affinity Capture-MS Homo sapiens
33 C6orf203  
Affinity Capture-MS Homo sapiens
34 PNKP 11284
Affinity Capture-MS Homo sapiens
35 NCOR2  
Affinity Capture-MS Homo sapiens
36 CDRT15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NTRK1 4914
Affinity Capture-MS Homo sapiens
38 PPP2CA 5515
Co-fractionation Homo sapiens
39 GTF3C1  
Co-fractionation Homo sapiens
40 LYRM4 57128
Affinity Capture-MS Homo sapiens
41 TMEM184A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CLK3  
Affinity Capture-MS Homo sapiens
43 DEF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CT45A5  
Affinity Capture-MS Homo sapiens
45 DNAJC7 7266
Co-fractionation Homo sapiens
46 CISD3 284106
Affinity Capture-MS Homo sapiens
47 STK3 6788
Affinity Capture-Western Homo sapiens
48 WBSCR16  
Affinity Capture-MS Homo sapiens
49 RPL10 6134
Affinity Capture-MS Homo sapiens
50 AURKAIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MSH2 4436
Co-fractionation Homo sapiens
52 HIST2H3C 126961
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
53 BOLA3  
Affinity Capture-MS Homo sapiens
54 MAD1L1  
Affinity Capture-MS Homo sapiens
55 PCDHB15  
Affinity Capture-MS Homo sapiens
56 ANKEF1  
Affinity Capture-MS Homo sapiens
57 PDGFRA 5156
Affinity Capture-MS Homo sapiens
58 ELAC2 60528
Co-fractionation Homo sapiens
59 UACA 55075
Affinity Capture-MS Homo sapiens
60 BATF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 OCIAD1 54940
Affinity Capture-MS Homo sapiens
62 NXF1 10482
Affinity Capture-RNA Homo sapiens
63 ERP44 23071
Affinity Capture-MS Homo sapiens
64 NRD1 4898
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 APOOL 139322
Affinity Capture-MS Homo sapiens
67 H2AFB1  
Affinity Capture-MS Homo sapiens
68 TBL1X 6907
Affinity Capture-Western Homo sapiens
69 HP1BP3 50809
Co-fractionation Homo sapiens
70 MOB2 81532
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 LPIN2  
Affinity Capture-MS Homo sapiens
72 CCDC140  
Affinity Capture-MS Homo sapiens
73 PUS1 80324
Co-fractionation Homo sapiens
74 PFDN5 5204
Co-fractionation Homo sapiens
75 FMR1NB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 Smchd1  
Affinity Capture-MS Mus musculus
77 ZRANB1 54764
Affinity Capture-MS Homo sapiens
78 ZNF707  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 SETSIP 646817
Co-fractionation Homo sapiens
81 TP53 7157
Affinity Capture-MS Homo sapiens
82 COQ6 51004
Affinity Capture-MS Homo sapiens
83 NME4 4833
Affinity Capture-MS Homo sapiens
84 HBEGF 1839
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
85 NLGN3  
Two-hybrid Homo sapiens
86 PFDN1 5201
Co-fractionation Homo sapiens
87 MOB1A 55233
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
88 ADCK2  
Affinity Capture-MS Homo sapiens
89 YARS 8565
Co-fractionation Homo sapiens
90 COQ7  
Affinity Capture-MS Homo sapiens
91 HSPB1 3315
Co-fractionation Homo sapiens
92 OPA3 80207
Affinity Capture-MS Homo sapiens
93 TNFSF12 8742
Affinity Capture-MS Homo sapiens
94 GNL1 2794
Co-fractionation Homo sapiens
95 IDE 3416
Negative Genetic Homo sapiens
96 XPO5 57510
Co-fractionation Homo sapiens
97 PPP1R12A 4659
Co-fractionation Homo sapiens
98 MOV10 4343
Affinity Capture-RNA Homo sapiens
99 DHRS4 10901
Affinity Capture-MS Homo sapiens
100 LMO1  
Affinity Capture-MS Homo sapiens
101 RPL36AL 6166
Affinity Capture-MS Homo sapiens
102 PPP2R2A 5520
Co-fractionation Homo sapiens
103 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NRD1 is involved
No pathways found





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