Gene description for LYRM4
Gene name LYR motif containing 4
Gene symbol LYRM4
Other names/aliases C6orf149
CGI-203
COXPD19
ISD11
Species Homo sapiens
 Database cross references - LYRM4
ExoCarta ExoCarta_57128
Vesiclepedia VP_57128
Entrez Gene 57128
HGNC 21365
MIM 613311
UniProt Q9HD34  
 LYRM4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for LYRM4
Molecular Function
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    iron-sulfur cluster assembly GO:0016226 IBA
    iron-sulfur cluster assembly GO:0016226 NAS
    [2Fe-2S] cluster assembly GO:0044571 IDA
    [4Fe-4S] cluster assembly GO:0044572 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 NAS
    mitochondrial matrix GO:0005759 TAS
    nuclear body GO:0016604 IDA
    mitochondrial [2Fe-2S] assembly complex GO:0099128 IDA
    L-cysteine desulfurase complex GO:1990221 IBA
    iron-sulfur cluster assembly complex GO:1990229 NAS
 Experiment description of studies that identified LYRM4 in sEVs
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for LYRM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFAB1 4706
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 ZWINT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ECH1 1891
Affinity Capture-MS Homo sapiens
5 FOXA1  
Affinity Capture-MS Homo sapiens
6 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PMPCB 9512
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TACO1  
Proximity Label-MS Homo sapiens
9 TSFM 10102
Proximity Label-MS Homo sapiens
10 AUH 549
Proximity Label-MS Homo sapiens
11 VWA8 23078
Proximity Label-MS Homo sapiens
12 MRRF  
Proximity Label-MS Homo sapiens
13 TBRG4 9238
Proximity Label-MS Homo sapiens
14 MRPL11 65003
Proximity Label-MS Homo sapiens
15 MTERF3  
Proximity Label-MS Homo sapiens
16 MTIF2 4528
Proximity Label-MS Homo sapiens
17 DDAH1 23576
Affinity Capture-MS Homo sapiens
18 FXN  
Affinity Capture-MS Homo sapiens
19 SDHA 6389
Co-fractionation Homo sapiens
20 Pcgf1  
Affinity Capture-MS Mus musculus
21 MDH2 4191
Proximity Label-MS Homo sapiens
22 CLPP 8192
Proximity Label-MS Homo sapiens
23 HOGA1  
Affinity Capture-MS Homo sapiens
24 TRUB2  
Proximity Label-MS Homo sapiens
25 CARTPT  
Affinity Capture-MS Homo sapiens
26 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
27 C6orf203  
Proximity Label-MS Homo sapiens
28 EPS8 2059
Affinity Capture-MS Homo sapiens
29 CHCHD2  
Affinity Capture-MS Homo sapiens
30 FASTKD5  
Proximity Label-MS Homo sapiens
31 ISCU 23479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 METTL15  
Proximity Label-MS Homo sapiens
33 C21orf33  
Proximity Label-MS Homo sapiens
34 CDNF  
Affinity Capture-MS Homo sapiens
35 SSBP1 6742
Proximity Label-MS Homo sapiens
36 UBA1 7317
Affinity Capture-MS Homo sapiens
37 TUFM 7284
Proximity Label-MS Homo sapiens
38 EFCAB13  
Affinity Capture-MS Homo sapiens
39 IDE 3416
Affinity Capture-MS Homo sapiens
40 PVRL2 5819
Two-hybrid Homo sapiens
41 HSCB 150274
Proximity Label-MS Homo sapiens
42 HSPD1 3329
Co-fractionation Homo sapiens
43 C1QBP 708
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GFM1 85476
Proximity Label-MS Homo sapiens
45 NRD1 4898
Affinity Capture-MS Homo sapiens
46 TFAM 7019
Proximity Label-MS Homo sapiens
47 FBXO2 26232
Affinity Capture-MS Homo sapiens
48 PMPCA 23203
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RAB24 53917
Affinity Capture-MS Homo sapiens
50 S100P 6286
Affinity Capture-MS Homo sapiens
51 NFS1 9054
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ACAD9 28976
Proximity Label-MS Homo sapiens
54 HINT2 84681
Proximity Label-MS Homo sapiens
55 KRAS 3845
Negative Genetic Homo sapiens
56 LYRM4 57128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 METTL17  
Proximity Label-MS Homo sapiens
58 GPX7 2882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 MTRF1L  
Proximity Label-MS Homo sapiens
60 HSPA9 3313
Co-fractionation Homo sapiens
61 MRPS26 64949
Proximity Label-MS Homo sapiens
62 BCKDHA 593
Affinity Capture-MS Homo sapiens
63 EXD2  
Proximity Label-MS Homo sapiens
64 MTRF1  
Proximity Label-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here