Experiment description of studies that identified WDR74 in exosomes

1 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for WDR74

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GNL2 29889 Co-fractionation Homo sapiens 2 RPSA 3921 Affinity Capture-MS Homo sapiens 3 RIT1 6016 Negative Genetic Homo sapiens 4 VPS35 55737 Affinity Capture-MS Homo sapiens 5 ARHGEF19   Affinity Capture-MS Homo sapiens 6 CCT3 7203 Affinity Capture-MS Homo sapiens 7 CIB2   Affinity Capture-MS Homo sapiens 8 RPL30 6156 Affinity Capture-MS Homo sapiens 9 FGD1   Affinity Capture-MS Homo sapiens 10 RPL13A 23521 Affinity Capture-MS Homo sapiens 11 NOP56 10528 Affinity Capture-MS Homo sapiens 12 SSB 6741 Co-fractionation Homo sapiens 13 CARD8   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 DNAJB6 10049 Proximity Label-MS Homo sapiens 15 USP36   Affinity Capture-MS Homo sapiens 16 RBM3 5935 Affinity Capture-MS Homo sapiens 17 D2HGDH 728294 Affinity Capture-MS Homo sapiens 18 SH3BP1 23616 Affinity Capture-MS Homo sapiens 19 NUDT3 11165 Two-hybrid Homo sapiens 20 NPM1 4869 Affinity Capture-MS Homo sapiens 21 SRP14 6727 Affinity Capture-MS Homo sapiens 22 RBM4B   Affinity Capture-MS Homo sapiens 23 PRKRA 8575 Affinity Capture-MS Homo sapiens 24 ATG16L1 55054 Affinity Capture-MS Homo sapiens 25 VAT1 10493 Co-fractionation Homo sapiens 26 Gspt1 14852 Affinity Capture-MS Mus musculus 27 PKN2 5586 Affinity Capture-MS Homo sapiens 28 MYCN   Affinity Capture-MS Homo sapiens 29 NPAS1   Affinity Capture-MS Homo sapiens 30 USP7 7874 Co-fractionation Homo sapiens 31 MAK16   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 ARHGEF4   Affinity Capture-MS Homo sapiens 33 RPL19 6143 Affinity Capture-MS Homo sapiens 34 KIF14 9928 Affinity Capture-MS Homo sapiens 35 CCT2 10576 Affinity Capture-MS Homo sapiens 36 Nvl   Affinity Capture-Western Mus musculus 37 NSUN2 54888 Co-fractionation Homo sapiens 38 RGS2   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 39 RPL4 6124 Affinity Capture-MS Homo sapiens 40 B4GALT2   Affinity Capture-MS Homo sapiens 41 C17orf85   Affinity Capture-MS Homo sapiens 42 CCT5 22948 Affinity Capture-MS Homo sapiens 43 PPIP5K2 23262 Co-fractionation Homo sapiens 44 SRSF6 6431 Affinity Capture-MS Homo sapiens 45 Srp72   Affinity Capture-MS Mus musculus 46 LRRC29   Affinity Capture-MS Homo sapiens 47 HIST1H1E 3008 Affinity Capture-MS Homo sapiens 48 LYAR 55646 Affinity Capture-MS Homo sapiens 49 HECTD1 25831 Affinity Capture-MS Homo sapiens 50 RPLP0 6175 Affinity Capture-MS Homo sapiens 51 SRPK3   Affinity Capture-MS Homo sapiens 52 MAGEB2 4113 Affinity Capture-MS Homo sapiens 53 DDX39B 7919 Co-fractionation Homo sapiens 54 ZC3H15 55854 Co-fractionation Homo sapiens 55 MYBBP1A 10514 Affinity Capture-MS Homo sapiens 56 CEBPA   Protein-peptide Homo sapiens 57 RPF1   Affinity Capture-MS Homo sapiens 58 Brwd3   Affinity Capture-MS Mus musculus 59 FGFBP1 9982 Affinity Capture-MS Homo sapiens 60 XPO1 7514 Affinity Capture-MS Homo sapiens 61 RPL35 11224 Affinity Capture-MS Homo sapiens 62 WBSCR22   Co-fractionation Homo sapiens 63 PDGFRA 5156 Affinity Capture-MS Homo sapiens 64 NPM3 10360 Affinity Capture-MS Homo sapiens 65 SRSF5 6430 Affinity Capture-MS Homo sapiens 66 RPL3 6122 Affinity Capture-MS Homo sapiens 67 EGFR 1956 Negative Genetic Homo sapiens 68 THOC5 8563 Affinity Capture-MS Homo sapiens 69 CCNJL   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 DHX37   Co-fractionation Homo sapiens 71 ZC3H10   Affinity Capture-MS Homo sapiens 72 ARHGAP40   Affinity Capture-MS Homo sapiens 73 MYC   Affinity Capture-MS Homo sapiens 74 DDX47 51202 Co-fractionation Homo sapiens 75 ARHGEF5   Affinity Capture-MS Homo sapiens 76 DNAJC9 23234 Proximity Label-MS Homo sapiens 77 METTL21B   Affinity Capture-MS Homo sapiens 78 HIST1H1A 3024 Affinity Capture-MS Homo sapiens 79 Junb   Affinity Capture-MS Mus musculus 80 RPL11 6135 Affinity Capture-MS Homo sapiens 81 CCDC140   Affinity Capture-MS Homo sapiens 82 ZC3H3   Affinity Capture-MS Homo sapiens 83 RPL13 6137 Affinity Capture-MS Homo sapiens 84 PUS7 54517 Co-fractionation Homo sapiens 85 PDCL3 79031 Affinity Capture-MS Homo sapiens 86 RRS1 23212 Co-fractionation Homo sapiens 87 BOP1 23246 Affinity Capture-MS Homo sapiens 88 DGCR8   Affinity Capture-MS Homo sapiens 89 RCHY1   Two-hybrid Homo sapiens 90 MKI67   Affinity Capture-MS Homo sapiens 91 CCT7 10574 Affinity Capture-MS Homo sapiens 92 UBE3C 9690 Co-fractionation Homo sapiens 93 NEIL1   Affinity Capture-MS Homo sapiens 94 IBTK 25998 Affinity Capture-MS Homo sapiens 95 RPL7A 6130 Affinity Capture-MS Homo sapiens 96 Rrbp1   Affinity Capture-MS Mus musculus 97 RPL28 6158 Affinity Capture-MS Homo sapiens 98 KRAS 3845 Synthetic Lethality Homo sapiens Negative Genetic Homo sapiens 99 ARHGAP23 57636 Affinity Capture-MS Homo sapiens 100 RPL17 6139 Affinity Capture-MS Homo sapiens View the network

Pathways in which WDR74 is involved

No pathways found