Gene description for WDR74
Gene name WD repeat domain 74
Gene symbol WDR74
Other names/aliases -
Species Homo sapiens
 Database cross references - WDR74
ExoCarta ExoCarta_54663
Entrez Gene 54663
HGNC 25529
UniProt Q6RFH5  
 WDR74 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for WDR74
Molecular Function
    protein binding GO:0005515 IPI
    molecular_function GO:0003674 ND
Biological Process
    blastocyst formation GO:0001825 IEA
    biological_process GO:0008150 ND
    RNA metabolic process GO:0016070 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified WDR74 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for WDR74
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RGS2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which WDR74 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here