Gene description for KALRN
Gene name kalirin, RhoGEF kinase
Gene symbol KALRN
Other names/aliases ARHGEF24
CHD5
CHDS5
DUET
DUO
HAPIP
TRAD
Species Homo sapiens
 Database cross references - KALRN
ExoCarta ExoCarta_8997
Entrez Gene 8997
HGNC 4814
MIM 604605
UniProt O60229  
 KALRN identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for KALRN
Molecular Function
    metal ion binding GO:0046872 IEA
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 EXP
    protein serine/threonine kinase activity GO:0004674 IEA
Biological Process
    vesicle-mediated transport GO:0016192 TAS
    positive regulation of GTPase activity GO:0043547 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    intracellular signal transduction GO:0035556 ISS
    axon guidance GO:0007411 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    apoptotic signaling pathway GO:0097190 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    ephrin receptor signaling pathway GO:0048013 TAS
    signal transduction GO:0007165 TAS
    nervous system development GO:0007399 ISS
    protein phosphorylation GO:0006468 IEA
Subcellular Localization
    actin cytoskeleton GO:0015629 TAS
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified KALRN in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for KALRN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PAM 5066
Two-hybrid Homo sapiens
View the network image/svg+xml



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