Gene description for PAM
Gene name peptidylglycine alpha-amidating monooxygenase
Gene symbol PAM
Other names/aliases PAL
PHM
Species Homo sapiens
 Database cross references - PAM
ExoCarta ExoCarta_5066
Vesiclepedia VP_5066
Entrez Gene 5066
HGNC 8596
MIM 170270
UniProt P19021  
 PAM identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Prostate cancer cells 25857301    
Saliva 19199708    
 Gene ontology annotations for PAM
Molecular Function
    peptidylglycine monooxygenase activity GO:0004504 IBA
    peptidylglycine monooxygenase activity GO:0004504 IDA
    peptidylamidoglycolate lyase activity GO:0004598 IDA
    peptidylamidoglycolate lyase activity GO:0004598 ISS
    copper ion binding GO:0005507 ISS
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    L-ascorbic acid binding GO:0031418 IEA
Biological Process
    peptide amidation GO:0001519 IDA
    response to zinc ion GO:0010043 IDA
    fatty acid primary amide biosynthetic process GO:0062112 ISS
Subcellular Localization
    extracellular region GO:0005576 IBA
    membrane GO:0016020 HDA
    transport vesicle membrane GO:0030658 IEA
    secretory granule membrane GO:0030667 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PAM in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 250
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|TSG101|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors "Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D"
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for PAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBR2 23304
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CCL3  
Affinity Capture-MS Homo sapiens
3 SERPINA6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PRG2  
Affinity Capture-MS Homo sapiens
5 BTNL2  
Affinity Capture-MS Homo sapiens
6 TMEM106A  
Affinity Capture-MS Homo sapiens
7 ART3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KLK15  
Affinity Capture-MS Homo sapiens
9 CEACAM8 1088
Affinity Capture-MS Homo sapiens
10 TSC2 7249
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
11 LILRB4  
Affinity Capture-MS Homo sapiens
12 RALB 5899
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CFC1  
Affinity Capture-MS Homo sapiens
16 DPEP2  
Affinity Capture-MS Homo sapiens
17 GLP1R  
PCA Homo sapiens
18 HS2ST1 9653
Affinity Capture-MS Homo sapiens
19 CBLN4  
Affinity Capture-MS Homo sapiens
20 ADPGK 83440
Affinity Capture-MS Homo sapiens
21 CALM1 801
Reconstituted Complex Homo sapiens
22 GAA 2548
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TSC1 7248
Co-localization Homo sapiens
24 IL5RA  
Affinity Capture-MS Homo sapiens
25 SUSD4  
Affinity Capture-MS Homo sapiens
26 SFTPC  
Affinity Capture-MS Homo sapiens
27 CLEC12B  
Affinity Capture-MS Homo sapiens
28 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
29 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 B3GNT2 10678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 SCGB2A2  
Affinity Capture-MS Homo sapiens
32 RASSF9  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
33 KALRN 8997
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 KRAS 3845
Synthetic Lethality Homo sapiens
35 FAM19A2 338811
Affinity Capture-MS Homo sapiens
36 KLRC1  
Affinity Capture-MS Homo sapiens
37 ST14 6768
Affinity Capture-MS Homo sapiens
38 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TRAV20  
Affinity Capture-MS Homo sapiens
40 PDGFRA 5156
Affinity Capture-MS Homo sapiens
41 C1QTNF7  
Affinity Capture-MS Homo sapiens
42 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PAM is involved
No pathways found





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