Gene description for KPNA6
Gene name karyopherin alpha 6 (importin alpha 7)
Gene symbol KPNA6
Other names/aliases IPOA7
KPNA7
Species Homo sapiens
 Database cross references - KPNA6
ExoCarta ExoCarta_23633
Vesiclepedia VP_23633
Entrez Gene 23633
HGNC 6399
MIM 610563
UniProt O60684  
 KPNA6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for KPNA6
Molecular Function
    protein binding GO:0005515 IPI
    nuclear localization sequence binding GO:0008139 IBA
    nuclear import signal receptor activity GO:0061608 IBA
Biological Process
    transcription by RNA polymerase II GO:0006366 IEA
    protein import into nucleus GO:0006606 ISS
    NLS-bearing protein import into nucleus GO:0006607 IBA
    viral genome replication GO:0019079 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    maternal process involved in female pregnancy GO:0060135 IEA
    entry of viral genome into host nucleus through nuclear pore complex via importin GO:0075506 IMP
    positive regulation of cytokine production involved in inflammatory response GO:1900017 IEA
    positive regulation of viral life cycle GO:1903902 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 NAS
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    NLS-dependent protein nuclear import complex GO:0042564 IPI
    host cell GO:0043657 IEA
 Experiment description of studies that identified KPNA6 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KPNA6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 NCBP1 4686
Affinity Capture-MS Homo sapiens
3 KPNA3 3839
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
4 NOP58 51602
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
5 UBE2O 63893
Affinity Capture-MS Homo sapiens
6 CDKN1B 1027
Reconstituted Complex Homo sapiens
7 ANP32A 8125
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 KPNA1 3836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TNPO1 3842
Co-fractionation Homo sapiens
10 KPNB1 3837
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
11 ACTC1 70
Proximity Label-MS Homo sapiens
12 NOP56 10528
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
13 MORF4L2  
Co-fractionation Homo sapiens
14 TRIM33 51592
Affinity Capture-MS Homo sapiens
15 RNMT 8731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 XRN2 22803
Affinity Capture-MS Homo sapiens
17 KPNA2 3838
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
18 Oxnad1  
Affinity Capture-MS Mus musculus
19 HDAC1 3065
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
20 SURF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CENPU  
Affinity Capture-MS Homo sapiens
22 PARK2  
Affinity Capture-MS Homo sapiens
23 TAF9 6880
Two-hybrid Homo sapiens
24 GFPT1 2673
Co-fractionation Homo sapiens
25 MCM2 4171
Affinity Capture-MS Homo sapiens
26 C18orf25 147339
Affinity Capture-MS Homo sapiens
27 NUP153 9972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CDK2AP1  
Affinity Capture-MS Homo sapiens
29 ANP32B 10541
Affinity Capture-MS Homo sapiens
30 HDAC2 3066
Affinity Capture-MS Homo sapiens
31 MTHFR 4524
Affinity Capture-MS Homo sapiens
32 FBL 2091
Co-fractionation Homo sapiens
33 POLR1E  
Affinity Capture-MS Homo sapiens
34 PSMC2 5701
Co-fractionation Homo sapiens
35 EXOSC4 54512
Co-fractionation Homo sapiens
36 Nhp2l1 20826
Affinity Capture-MS Mus musculus
37 FAS 355
Proximity Label-MS Homo sapiens
38 UNK  
Affinity Capture-RNA Homo sapiens
39 PPM1D  
Affinity Capture-MS Homo sapiens
40 TADA2B  
Affinity Capture-MS Homo sapiens
41 MEPCE 56257
Affinity Capture-MS Homo sapiens
42 MAP1B 4131
Co-fractionation Homo sapiens
43 NONO 4841
Affinity Capture-MS Homo sapiens
44 NOP2 4839
Two-hybrid Homo sapiens
45 SNRNP200 23020
Co-fractionation Homo sapiens
46 Ctnnbl1  
Affinity Capture-MS Mus musculus
47 CHMP4B 128866
Affinity Capture-MS Homo sapiens
48 SP110  
Affinity Capture-MS Homo sapiens
49 RAN 5901
Co-fractionation Homo sapiens
50 Ddx21 56200
Affinity Capture-MS Mus musculus
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 Mki67 17345
Affinity Capture-MS Mus musculus
53 ZNF131  
Reconstituted Complex Homo sapiens
54 FAM103A1  
Affinity Capture-MS Homo sapiens
55 LYAR 55646
Affinity Capture-MS Homo sapiens
56 COPS3 8533
Co-fractionation Homo sapiens
57 GSG2  
Affinity Capture-MS Homo sapiens
58 RANBP6 26953
Co-fractionation Homo sapiens
59 PPM1G 5496
Affinity Capture-MS Homo sapiens
60 BZW1 9689
Co-fractionation Homo sapiens
61 RPL11 6135
Co-fractionation Homo sapiens
62 OGT 8473
Reconstituted Complex Homo sapiens
63 IPO5 3843
Co-fractionation Homo sapiens
64 NR4A1  
Affinity Capture-MS Homo sapiens
65 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 MUS81  
Synthetic Growth Defect Homo sapiens
67 LMNB1 4001
Affinity Capture-MS Homo sapiens
68 NCBP2 22916
Affinity Capture-MS Homo sapiens
69 CD2BP2 10421
Affinity Capture-MS Homo sapiens
70 PRKAR2A 5576
Co-fractionation Homo sapiens
71 SKP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
72 METTL16 79066
Affinity Capture-MS Homo sapiens
73 VARS 7407
Co-fractionation Homo sapiens
74 MORF4L1  
Co-fractionation Homo sapiens
75 NAF1  
Affinity Capture-MS Homo sapiens
76 NT5C2 22978
Co-fractionation Homo sapiens
77 FRG1B  
Two-hybrid Homo sapiens
78 Mast3  
Affinity Capture-MS Mus musculus
79 PSPC1 55269
Affinity Capture-MS Homo sapiens
80 RPA3 6119
Proximity Label-MS Homo sapiens
81 TTC1 7265
Co-fractionation Homo sapiens
82 CHD8 57680
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 ANP32E 81611
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 LMNA 4000
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
85 MDM2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
86 Nipbl  
Affinity Capture-MS Mus musculus
87 IL1A  
Two-hybrid Homo sapiens
88 COPS2 9318
Co-fractionation Homo sapiens
89 HIF1A 3091
Reconstituted Complex Homo sapiens
90 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
91 PHB2 11331
Affinity Capture-Western Homo sapiens
92 SPOP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 SIRT6  
Affinity Capture-MS Homo sapiens
94 CENPA  
Proximity Label-MS Homo sapiens
95 ILF3 3609
Affinity Capture-MS Homo sapiens
96 HMGB1 3146
Co-fractionation Homo sapiens
97 KEAP1 9817
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
98 IKBKG 8517
Affinity Capture-Western Homo sapiens
99 Chmp2a 68953
Affinity Capture-MS Mus musculus
100 PTTG1  
Synthetic Growth Defect Homo sapiens
101 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
102 PTPN2 5771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 CREBBP  
Biochemical Activity Homo sapiens
104 CHD4 1108
Affinity Capture-MS Homo sapiens
105 KLHL7  
Affinity Capture-MS Homo sapiens
106 SIRT1  
Affinity Capture-MS Homo sapiens
107 CACUL1  
Affinity Capture-MS Homo sapiens
108 NUP50 10762
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 SREK1IP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 C16orf72 29035
Affinity Capture-MS Homo sapiens
111 RELB  
Affinity Capture-MS Homo sapiens
112 MEAF6  
Affinity Capture-MS Homo sapiens
113 Ufl1  
Affinity Capture-MS Mus musculus
114 C9orf72  
Affinity Capture-MS Homo sapiens
115 KBTBD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 RAC1 5879
Two-hybrid Homo sapiens
117 MEN1 4221
Affinity Capture-MS Homo sapiens
118 FOXE1  
Affinity Capture-MS Homo sapiens
119 PRMT1 3276
Co-fractionation Homo sapiens
120 ALKBH5 54890
Affinity Capture-MS Homo sapiens
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