Gene description for Chmp2a
Gene name charged multivesicular body protein 2A
Gene symbol Chmp2a
Other names/aliases 1500016L11Rik
Species Mus musculus
 Database cross references - Chmp2a
ExoCarta ExoCarta_68953
Vesiclepedia VP_68953
Entrez Gene 68953
UniProt Q9DB34  
 Chmp2a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Chmp2a
Molecular Function
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    phosphatidylcholine binding GO:0031210 IEA
    phosphatidylcholine binding GO:0031210 ISO
Biological Process
    plasma membrane repair GO:0001778 ISO
    autophagy GO:0006914 ISO
    nucleus organization GO:0006997 ISO
    mitotic metaphase chromosome alignment GO:0007080 ISO
    membrane invagination GO:0010324 IEA
    membrane invagination GO:0010324 ISO
    exit from mitosis GO:0010458 ISO
    exit from mitosis GO:0010458 ISS
    regulation of centrosome duplication GO:0010824 IEA
    regulation of centrosome duplication GO:0010824 ISO
    protein transport GO:0015031 IBA
    protein transport GO:0015031 IEA
    viral release from host cell GO:0019076 ISO
    nuclear membrane reassembly GO:0031468 ISO
    nuclear membrane reassembly GO:0031468 ISS
    endosome transport via multivesicular body sorting pathway GO:0032509 IBA
    multivesicular body assembly GO:0036258 NAS
    viral budding via host ESCRT complex GO:0039702 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    establishment of protein localization GO:0045184 ISO
    late endosome to vacuole transport GO:0045324 IBA
    viral budding from plasma membrane GO:0046761 ISO
    protein polymerization GO:0051258 IEA
    protein polymerization GO:0051258 ISO
    protein homooligomerization GO:0051260 IEA
    protein homooligomerization GO:0051260 ISO
    vesicle fusion with vacuole GO:0051469 NAS
    multivesicular body-lysosome fusion GO:0061763 NAS
    midbody abscission GO:0061952 ISO
    multivesicular body sorting pathway GO:0071985 ISO
    membrane fission GO:0090148 NAS
    autophagosome maturation GO:0097352 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    late endosome to lysosome transport GO:1902774 ISO
    positive regulation of exosomal secretion GO:1903543 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
    negative regulation of centriole elongation GO:1903723 IEA
    negative regulation of centriole elongation GO:1903723 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 ISO
    kinetochore GO:0000776 ISO
    chromatin GO:0000785 IEA
    chromatin GO:0000785 ISO
    ESCRT III complex GO:0000815 IBA
    ESCRT III complex GO:0000815 IEA
    ESCRT III complex GO:0000815 ISO
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISS
    nuclear pore GO:0005643 ISO
    lysosomal membrane GO:0005765 ISO
    multivesicular body GO:0005771 IBA
    kinetochore microtubule GO:0005828 ISO
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 ISO
    membrane coat GO:0030117 IEA
    membrane coat GO:0030117 ISO
    midbody GO:0030496 ISO
    multivesicular body membrane GO:0032585 ISO
    amphisome membrane GO:1904930 ISO
 Experiment description of studies that identified Chmp2a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Chmp2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Vps4b 20479
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
3 2610002M06Rik  
Co-fractionation Mus musculus
4 Chmp1a  
Co-fractionation Mus musculus
5 MEN1 4221
Affinity Capture-MS Homo sapiens
6 CHMP1A 5119
Affinity Capture-MS Homo sapiens
7 Eed  
Affinity Capture-MS Mus musculus
8 RBM26 64062
Affinity Capture-MS Homo sapiens
9 CHMP3 51652
Affinity Capture-MS Homo sapiens
10 Fitm2  
Negative Genetic Mus musculus
11 Chmp2b  
Co-fractionation Mus musculus
12 NES 10763
Affinity Capture-MS Homo sapiens
13 Mitd1 69028
Co-fractionation Mus musculus
14 Chmp3  
Co-fractionation Mus musculus
15 CLU 1191
Affinity Capture-MS Homo sapiens
16 KPNA6 23633
Affinity Capture-MS Homo sapiens
17 CHMP4B 128866
Affinity Capture-MS Homo sapiens
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