Gene description for Chmp2a
Gene name charged multivesicular body protein 2A
Gene symbol Chmp2a
Other names/aliases 1500016L11Rik
Species Mus musculus
 Database cross references - Chmp2a
ExoCarta ExoCarta_68953
Entrez Gene 68953
UniProt Q9DB34  
 Chmp2a identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Chmp2a
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylcholine binding GO:0031210 ISO
    protein domain specific binding GO:0019904 ISO
Biological Process
    transport GO:0006810 IEA
    membrane invagination GO:0010324 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    viral budding via host ESCRT complex GO:0039702 ISO
    positive regulation of viral release from host cell GO:1902188 ISO
    establishment of protein localization GO:0045184 ISO
    regulation of viral process GO:0050792 ISO
    cell separation after cytokinesis GO:0000920 ISO
    regulation of centrosome duplication GO:0010824 ISO
    positive regulation of exosomal secretion GO:1903543 ISO
    mitotic metaphase plate congression GO:0007080 ISO
    negative regulation of cell death GO:0060548 ISO
    protein transport GO:0015031 IEA
    vacuolar transport GO:0007034 IEA
    nucleus organization GO:0006997 ISO
    protein homooligomerization GO:0051260 ISO
    protein polymerization GO:0051258 ISO
    protein heterooligomerization GO:0051291 ISO
    negative regulation of centriole elongation GO:1903723 ISO
Subcellular Localization
    membrane coat GO:0030117 ISO
    cytosol GO:0005829 IDA
    endosome GO:0005768 IEA
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Chmp2a in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Chmp2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Chmp2a is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome
Lysosome Vesicle Biogenesis IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here