Gene description for ASPSCR1
Gene name alveolar soft part sarcoma chromosome region, candidate 1
Gene symbol ASPSCR1
Other names/aliases ASPCR1
ASPL
ASPS
RCC17
TUG
UBXD9
UBXN9
Species Homo sapiens
 Database cross references - ASPSCR1
ExoCarta ExoCarta_79058
Vesiclepedia VP_79058
Entrez Gene 79058
HGNC 13825
MIM 606236
UniProt Q9BZE9  
 ASPSCR1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for ASPSCR1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    positive regulation of protein modification process GO:0031401 IMP
    glucose homeostasis GO:0042593 IBA
    regulation of D-glucose import GO:0046324 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IDA
    cytoplasmic side of plasma membrane GO:0009898 IEA
    endomembrane system GO:0012505 IEA
    vesicle membrane GO:0012506 IBA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified ASPSCR1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ASPSCR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SHKBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MAN2C1 4123
Affinity Capture-MS Homo sapiens
3 NOTCH2NL 388677
Two-hybrid Homo sapiens
4 NSFL1C 55968
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HACL1 26061
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 UFD1L 7353
Affinity Capture-MS Homo sapiens
7 XAF1  
Affinity Capture-MS Homo sapiens
8 ATPIF1 93974
Cross-Linking-MS (XL-MS) Homo sapiens
9 NPLOC4 55666
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 HK1 3098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NEDD4 4734
Reconstituted Complex Homo sapiens
12 FTL 2512
Affinity Capture-MS Homo sapiens
13 PREX1  
Affinity Capture-MS Homo sapiens
14 USP25  
Affinity Capture-Western Homo sapiens
15 NSF 4905
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
16 YTHDF2 51441
Co-fractionation Homo sapiens
17 URGCP 55665
Affinity Capture-MS Homo sapiens
18 YTHDF1 54915
Co-fractionation Homo sapiens
19 UBXN10  
Affinity Capture-MS Homo sapiens
20 ADAMTSL4  
Two-hybrid Homo sapiens
21 MAT1A 4143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 KRTAP10-8  
Two-hybrid Homo sapiens
23 CLK2 1196
Two-hybrid Homo sapiens
24 INCA1  
Two-hybrid Homo sapiens
25 SEPHS1 22929
Affinity Capture-MS Homo sapiens
26 PIPSL 266971
Affinity Capture-MS Homo sapiens
27 EFTUD2 9343
Affinity Capture-MS Homo sapiens
28 GLTP 51228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 UBC 7316
Affinity Capture-MS Homo sapiens
30 KRTAP1-1  
Two-hybrid Homo sapiens
31 DCAF11  
Co-fractionation Homo sapiens
32 PHAX  
Two-hybrid Homo sapiens
33 UBXN2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 DERA 51071
Co-fractionation Homo sapiens
35 FADS1 3992
Affinity Capture-MS Homo sapiens
36 APP 351
Reconstituted Complex Homo sapiens
37 PSMD14 10213
Affinity Capture-MS Homo sapiens
38 UBXN7 26043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ASNA1 439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TCF4  
Two-hybrid Homo sapiens
41 HPD 3242
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 EZH2  
Affinity Capture-MS Homo sapiens
43 GLUL 2752
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RPA3 6119
Proximity Label-MS Homo sapiens
45 NDOR1  
Affinity Capture-MS Homo sapiens
46 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
47 C16orf72 29035
Affinity Capture-MS Homo sapiens
48 NTRK1 4914
Affinity Capture-MS Homo sapiens
49 PINK1  
Affinity Capture-MS Homo sapiens
50 ALPP 250
Affinity Capture-MS Homo sapiens
51 SERPINB2 5055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DNAJC24  
Affinity Capture-MS Homo sapiens
53 GAPDH 2597
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 BANF1 8815
Co-fractionation Homo sapiens
55 VCPKMT  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
56 FAF1 11124
Affinity Capture-MS Homo sapiens
57 UBXN1 51035
Cross-Linking-MS (XL-MS) Homo sapiens
58 DDX58 23586
Affinity Capture-RNA Homo sapiens
59 VCPIP1 80124
Affinity Capture-MS Homo sapiens
60 AGL 178
Co-fractionation Homo sapiens
61 KCTD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 KRT31 3881
Two-hybrid Homo sapiens
63 UBXN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FAM136A 84908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SLC12A9 56996
Affinity Capture-MS Homo sapiens
66 HUWE1 10075
Affinity Capture-MS Homo sapiens
67 UBXN6 80700
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 TACC3  
Two-hybrid Homo sapiens
69 HAO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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