Gene description for HPD
Gene name 4-hydroxyphenylpyruvate dioxygenase
Gene symbol HPD
Other names/aliases 4-HPPD
4HPPD
GLOD3
HPPDASE
PPD
Species Homo sapiens
 Database cross references - HPD
ExoCarta ExoCarta_3242
Vesiclepedia VP_3242
Entrez Gene 3242
HGNC 5147
MIM 609695
UniProt P32754  
 HPD identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for HPD
Molecular Function
    4-hydroxyphenylpyruvate dioxygenase activity GO:0003868 IBA
    4-hydroxyphenylpyruvate dioxygenase activity GO:0003868 IDA
    4-hydroxyphenylpyruvate dioxygenase activity GO:0003868 ISS
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    L-phenylalanine catabolic process GO:0006559 IEA
    tyrosine catabolic process GO:0006572 IBA
    tyrosine catabolic process GO:0006572 IDA
    tyrosine catabolic process GO:0006572 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    endoplasmic reticulum membrane GO:0005789 IBA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified HPD in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HPD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ASPSCR1 79058
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 IKBKG 8517
Two-hybrid Homo sapiens
3 HIVEP1 3096
Two-hybrid Homo sapiens
4 CDKN1A  
Two-hybrid Homo sapiens
5 HRSP12 10247
Two-hybrid Homo sapiens
6 RTN4 57142
Affinity Capture-MS Homo sapiens
7 RBMX 27316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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