Gene description for SEPHS1
Gene name selenophosphate synthetase 1
Gene symbol SEPHS1
Other names/aliases SELD
SPS
SPS1
Species Homo sapiens
 Database cross references - SEPHS1
ExoCarta ExoCarta_22929
Vesiclepedia VP_22929
Entrez Gene 22929
HGNC 19685
MIM 600902
UniProt P49903  
 SEPHS1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SEPHS1
Molecular Function
    selenide, water dikinase activity GO:0004756 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    GTP binding GO:0005525 TAS
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 IDA
Biological Process
    selenocysteine biosynthetic process GO:0016260 IBA
    protein modification process GO:0036211 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IEA
    nuclear membrane GO:0031965 IDA
    nuclear membrane GO:0031965 IEA
 Experiment description of studies that identified SEPHS1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SEPHS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 PTPRF 5792
Co-fractionation Homo sapiens
3 PDCD6 10016
Co-fractionation Homo sapiens
4 SUGT1 10910
Co-fractionation Homo sapiens
5 CCDC130  
Affinity Capture-MS Homo sapiens
6 ECH1 1891
Co-fractionation Homo sapiens
7 PKLR 5313
Cross-Linking-MS (XL-MS) Homo sapiens
8 CLIC1 1192
Co-fractionation Homo sapiens
9 CDCA4 55038
Two-hybrid Homo sapiens
10 COPS2 9318
Co-fractionation Homo sapiens
11 GART 2618
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
12 TAS2R41  
Affinity Capture-MS Homo sapiens
13 C14orf1 11161
Two-hybrid Homo sapiens
14 AHCYL1 10768
Co-fractionation Homo sapiens
15 XPO1 7514
Co-fractionation Homo sapiens
16 ACADS 35
Co-fractionation Homo sapiens
17 SEC13 6396
Co-fractionation Homo sapiens
18 ASPSCR1 79058
Affinity Capture-MS Homo sapiens
19 DEPDC7  
Two-hybrid Homo sapiens
20 C9orf9  
Two-hybrid Homo sapiens
21 SEPHS1 22929
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 HYPK 25764
Co-fractionation Homo sapiens
23 CUL3 8452
Affinity Capture-MS Homo sapiens
24 HLX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SEPHS2  
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
26 PYCR1 5831
Co-fractionation Homo sapiens
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 IGSF21  
Two-hybrid Homo sapiens
29 SLC35F6 54978
Two-hybrid Homo sapiens
30 SAT1  
Two-hybrid Homo sapiens
31 MAT2B 27430
Co-fractionation Homo sapiens
32 GBP2  
Two-hybrid Homo sapiens
33 DDX19A 55308
Co-fractionation Homo sapiens
34 SOX2  
Affinity Capture-MS Homo sapiens
35 G6PD 2539
Co-fractionation Homo sapiens
36 TUFM 7284
Co-fractionation Homo sapiens
37 TPD52 7163
Co-fractionation Homo sapiens
38 PPIL3 53938
Co-fractionation Homo sapiens
39 XPO7 23039
Co-fractionation Homo sapiens
40 UMPS 7372
Co-fractionation Homo sapiens
41 QRICH1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
42 ANPEP 290
Co-fractionation Homo sapiens
43 RTCB 51493
Co-fractionation Homo sapiens
44 FDPS 2224
Co-fractionation Homo sapiens
45 CRKL 1399
Co-fractionation Homo sapiens
46 DIS3 22894
Co-fractionation Homo sapiens
47 PUM1 9698
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
48 REXO2 25996
Co-fractionation Homo sapiens
49 ZBTB25  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 UNC119 9094
Two-hybrid Homo sapiens
51 WDR12 55759
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
52 HSPB1 3315
Co-fractionation Homo sapiens
53 CLIC4 25932
Co-fractionation Homo sapiens
54 MCFD2 90411
Co-fractionation Homo sapiens
55 PIPSL 266971
Co-fractionation Homo sapiens
56 CRMP1 1400
Two-hybrid Homo sapiens
57 PFAS 5198
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which SEPHS1 is involved
No pathways found





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