Gene description for PPIL3
Gene name peptidylprolyl isomerase (cyclophilin)-like 3
Gene symbol PPIL3
Other names/aliases CYPJ
Species Homo sapiens
 Database cross references - PPIL3
ExoCarta ExoCarta_53938
Vesiclepedia VP_53938
Entrez Gene 53938
HGNC 9262
MIM 615811
UniProt Q9H2H8  
 PPIL3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PPIL3
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    protein binding GO:0005515 IPI
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IC
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein folding GO:0006457 IBA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified PPIL3 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPIL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Co-fractionation Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 SDHD  
Affinity Capture-MS Homo sapiens
4 BAG3 9531
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
5 WDR82 80335
Co-fractionation Homo sapiens
6 SUGT1 10910
Co-fractionation Homo sapiens
7 SNCA 6622
Affinity Capture-MS Homo sapiens
8 Cstf2t  
Two-hybrid Mus musculus
9 Cct4 12464
Affinity Capture-MS Mus musculus
10 FASN 2194
Negative Genetic Homo sapiens
11 SNX3 8724
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
12 ACP1 52
Co-fractionation Homo sapiens
13 COX5B 1329
Co-fractionation Homo sapiens
14 GLOD4 51031
Co-fractionation Homo sapiens
15 RCN1 5954
Co-fractionation Homo sapiens
16 SLU7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
17 SOD1 6647
Co-fractionation Homo sapiens
18 AHCYL1 10768
Co-fractionation Homo sapiens
19 UBE2H 7328
Affinity Capture-MS Homo sapiens
20 YTHDF1 54915
Two-hybrid Homo sapiens
21 ECT2 1894
Affinity Capture-MS Homo sapiens
22 CCDC132 55610
Affinity Capture-MS Homo sapiens
23 E2F4  
Affinity Capture-MS Homo sapiens
24 RPAP1  
Co-fractionation Homo sapiens
25 PLCL2 23228
Affinity Capture-MS Homo sapiens
26 RAD23B 5887
Co-fractionation Homo sapiens
27 SEPHS1 22929
Co-fractionation Homo sapiens
28 Spire2  
Affinity Capture-MS Mus musculus
29 Rbm14  
Two-hybrid Mus musculus
30 MTAP 4507
Co-fractionation Homo sapiens
31 GPALPP1  
Affinity Capture-MS Homo sapiens
32 CCDC84 338657
Two-hybrid Homo sapiens
33 DMRTB1  
Two-hybrid Homo sapiens
34 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
35 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
36 TNNC2  
Affinity Capture-MS Homo sapiens
37 ECI1 1632
Co-fractionation Homo sapiens
38 PHC1  
Two-hybrid Homo sapiens
39 PRSS12 8492
Affinity Capture-MS Homo sapiens
40 SERPINB5 5268
Co-fractionation Homo sapiens
41 MAPRE1 22919
Affinity Capture-MS Homo sapiens
42 BAG6 7917
Affinity Capture-MS Homo sapiens
43 NUDCD2 134492
Co-fractionation Homo sapiens
44 SGTB  
Affinity Capture-MS Homo sapiens
45 PRKACA 5566
Affinity Capture-MS Homo sapiens
46 PCBP1 5093
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 PSMA1 5682
Co-fractionation Homo sapiens
48 TAGLN2 8407
Co-fractionation Homo sapiens
49 BAG1 573
Co-fractionation Homo sapiens
50 RPS13 6207
Two-hybrid Homo sapiens
51 CCS 9973
Co-fractionation Homo sapiens
52 TMOD3 29766
Co-fractionation Homo sapiens
53 GOT1 2805
Co-fractionation Homo sapiens
54 CIT 11113
Affinity Capture-MS Homo sapiens
55 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
56 Srsf9  
Two-hybrid Mus musculus
57 SEC24C 9632
Co-fractionation Homo sapiens
58 SNX12 29934
Co-fractionation Homo sapiens
59 IL17F  
Affinity Capture-MS Homo sapiens
60 Cdca5  
Affinity Capture-MS Mus musculus
61 AR 367
Proximity Label-MS Homo sapiens
62 FAM49B 51571
Co-fractionation Homo sapiens
63 LMNA 4000
Affinity Capture-MS Homo sapiens
64 SP7  
Proximity Label-MS Homo sapiens
65 Slc6a8 102857
Affinity Capture-MS Mus musculus
66 CEBPA  
Proximity Label-MS Homo sapiens
67 GINS4  
Co-fractionation Homo sapiens
68 SUMO1 7341
Co-fractionation Homo sapiens
69 FABP7  
Co-fractionation Homo sapiens
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