Gene description for GNG3
Gene name guanine nucleotide binding protein (G protein), gamma 3
Gene symbol GNG3
Other names/aliases -
Species Homo sapiens
 Database cross references - GNG3
ExoCarta ExoCarta_2785
Vesiclepedia VP_2785
Entrez Gene 2785
HGNC 4405
MIM 608941
UniProt P63215  
 GNG3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GNG3
Molecular Function
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    G-protein beta-subunit binding GO:0031681 IBA
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
Subcellular Localization
    heterotrimeric G-protein complex GO:0005834 IBA
    plasma membrane GO:0005886 TAS
    postsynaptic density GO:0014069 IEA
    dendrite GO:0030425 IEA
    cell body GO:0044297 IEA
 Experiment description of studies that identified GNG3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GNG3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDCL 5082
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 GNB5 10681
FRET Homo sapiens
Two-hybrid Homo sapiens
4 GNAT2 2780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GNAZ 2781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GNA13 10672
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FLNC 2318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TRIM73  
Two-hybrid Homo sapiens
9 GNA11 2767
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 GNAQ 2776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GNAS 2778
Affinity Capture-MS Homo sapiens
12 GNB2 2783
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 GNB3 2784
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 GNB4 59345
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 GNAO1 2775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 GNB1 2782
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GNAI1 2770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GNAI2 2771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 WDYHV1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GNG3 is involved
PathwayEvidenceSource
Activation of G protein gated Potassium channels TAS Reactome
Activation of GABAB receptors TAS Reactome
Activation of kainate receptors upon glutamate binding TAS Reactome
ADORA2B mediated anti-inflammatory cytokines production TAS Reactome
ADP signalling through P2Y purinoceptor 1 TAS Reactome
ADP signalling through P2Y purinoceptor 1 IEA Reactome
ADP signalling through P2Y purinoceptor 12 TAS Reactome
Adrenaline,noradrenaline inhibits insulin secretion TAS Reactome
Adrenaline,noradrenaline inhibits insulin secretion IEA Reactome
Anti-inflammatory response favouring Leishmania parasite infection TAS Reactome
Aquaporin-mediated transport TAS Reactome
Beta-catenin independent WNT signaling TAS Reactome
Beta-catenin independent WNT signaling IEA Reactome
Ca2+ pathway TAS Reactome
Ca2+ pathway IEA Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
Chaperonin-mediated protein folding TAS Reactome
Class B/2 (Secretin family receptors) TAS Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding TAS Reactome
Disease TAS Reactome
ESR-mediated signaling TAS Reactome
Extra-nuclear estrogen signaling TAS Reactome
G alpha (12/13) signalling events TAS Reactome
G alpha (i) signalling events TAS Reactome
G alpha (q) signalling events TAS Reactome
G alpha (s) signalling events TAS Reactome
G alpha (z) signalling events TAS Reactome
G beta:gamma signalling through BTK TAS Reactome
G beta:gamma signalling through CDC42 TAS Reactome
G beta:gamma signalling through PI3Kgamma TAS Reactome
G beta:gamma signalling through PLC beta TAS Reactome
G protein gated Potassium channels TAS Reactome
G-protein activation TAS Reactome
G-protein beta:gamma signalling TAS Reactome
GABA B receptor activation TAS Reactome
GABA receptor activation TAS Reactome
Glucagon signaling in metabolic regulation TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Glucagon-type ligand receptors TAS Reactome
GPCR downstream signalling TAS Reactome
GPCR ligand binding TAS Reactome
GPER1 signaling TAS Reactome
Hemostasis TAS Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells TAS Reactome
Infectious disease TAS Reactome
Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits TAS Reactome
Integration of energy metabolism TAS Reactome
Integration of energy metabolism IEA Reactome
Inwardly rectifying K+ channels TAS Reactome
Leishmania infection TAS Reactome
Leishmania parasite growth and survival TAS Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of proteins TAS Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Opioid Signalling TAS Reactome
Parasitic Infection Pathways TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet homeostasis IEA Reactome
Platelet homeostasis TAS Reactome
Potassium Channels TAS Reactome
Presynaptic function of Kainate receptors TAS Reactome
Prostacyclin signalling through prostacyclin receptor IEA Reactome
Prostacyclin signalling through prostacyclin receptor TAS Reactome
Protein folding TAS Reactome
Regulation of insulin secretion TAS Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress TAS Reactome
Signal amplification TAS Reactome
Signal amplification IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) TAS Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Thromboxane signalling through TP receptor TAS Reactome
Transmission across Chemical Synapses TAS Reactome
Transport of small molecules TAS Reactome
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome





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