Gene description for GNB5
Gene name guanine nucleotide binding protein (G protein), beta 5
Gene symbol GNB5
Other names/aliases GB5
Species Homo sapiens
 Database cross references - GNB5
ExoCarta ExoCarta_10681
Entrez Gene 10681
HGNC 4401
MIM 604447
UniProt O14775  
 GNB5 identified in exosomes derived from the following tissue/cell type
Malignant pleural effusions 14975938    
 Gene ontology annotations for GNB5
Molecular Function
    protein binding GO:0005515 IPI
    chaperone binding GO:0051087 IPI
    GTPase activity GO:0003924 NAS
    G-protein gamma-subunit binding GO:0031682 IPI
    signal transducer activity GO:0004871 NAS
Biological Process
    signal transduction GO:0007165 NAS
    rhodopsin mediated signaling pathway GO:0016056 TAS
    metabolic process GO:0008152 NAS
    phototransduction, visible light GO:0007603 TAS
    regulation of rhodopsin mediated signaling pathway GO:0022400 TAS
    negative regulation of voltage-gated calcium channel activity GO:1901386 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified GNB5 in exosomes
1
Experiment ID 51
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
 Protein-protein interactions for GNB5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RGS7  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
2 GNG2 54331
Two-hybrid Homo sapiens
3 GNG4  
Two-hybrid Homo sapiens
4 GNG3  
Two-hybrid Homo sapiens
5 MVD 4597
Two-hybrid Homo sapiens
6 GNG7 2788
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
7 GNG5 2787
Two-hybrid Homo sapiens
8 MCM2 4171
Two-hybrid Homo sapiens
9 GNGT1  
Two-hybrid Homo sapiens
10 GNG13  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 N/A  
Two-hybrid 284812
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