Gene description for GNB5
Gene name guanine nucleotide binding protein (G protein), beta 5
Gene symbol GNB5
Other names/aliases GB5
Species Homo sapiens
 Database cross references - GNB5
ExoCarta ExoCarta_10681
Vesiclepedia VP_10681
Entrez Gene 10681
HGNC 4401
MIM 604447
UniProt O14775  
 GNB5 identified in sEVs derived from the following tissue/cell type
Malignant pleural effusions 14975938    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GNB5
Molecular Function
    GTPase activity GO:0003924 NAS
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    signaling receptor complex adaptor activity GO:0030159 IBA
    G-protein gamma-subunit binding GO:0031682 IPI
    protein-folding chaperone binding GO:0051087 IPI
Biological Process
    signal transduction GO:0007165 NAS
    G protein-coupled dopamine receptor signaling pathway GO:0007212 IBA
    G protein-coupled dopamine receptor signaling pathway GO:0007212 IDA
    light adaption GO:0036367 IEA
    positive regulation of GTPase activity GO:0043547 IDA
    negative regulation of voltage-gated calcium channel activity GO:1901386 IDA
    dark adaptation GO:1990603 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    heterotrimeric G-protein complex GO:0005834 IBA
    dendrite GO:0030425 IEA
    presynaptic membrane GO:0042734 IEA
    postsynaptic membrane GO:0045211 IEA
    cell tip GO:0051286 IEA
    parallel fiber to Purkinje cell synapse GO:0098688 IEA
    GTPase activator complex GO:1902773 TAS
 Experiment description of studies that identified GNB5 in sEVs
1
Experiment ID 51
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GNB5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNG2 54331
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
2 GNG13  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
3 CSNK2B 1460
Two-hybrid Homo sapiens
4 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MVD 4597
Two-hybrid Homo sapiens
6 GNG3 2785
Two-hybrid Homo sapiens
FRET Homo sapiens
7 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PDCL 5082
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 GNG4  
Two-hybrid Homo sapiens
10 ANXA7 310
Two-hybrid Homo sapiens
11 GNG8  
Affinity Capture-MS Homo sapiens
12 TCP1 6950
Affinity Capture-MS Homo sapiens
13 USP38 84640
Affinity Capture-MS Homo sapiens
14 BNIP3L  
Two-hybrid Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 CCT6B 10693
Affinity Capture-MS Homo sapiens
17 CDKN1A  
Two-hybrid Homo sapiens
18 PSMD4 5710
Two-hybrid Homo sapiens
19 FKBP8 23770
Affinity Capture-MS Homo sapiens
20 GNB1 2782
Affinity Capture-MS Homo sapiens
21 APP 351
Reconstituted Complex Homo sapiens
22 CCT7 10574
Affinity Capture-MS Homo sapiens
23 RGS7  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
24 CCT5 22948
Affinity Capture-Western Homo sapiens
25 RGS6 9628
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 E2F2 1870
Two-hybrid Homo sapiens
27 FOXK2  
Affinity Capture-MS Homo sapiens
28 CCT6A 908
Affinity Capture-MS Homo sapiens
29 RGS7BP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 TSC22D1 8848
Two-hybrid Homo sapiens
31 PRPF40B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MCM2 4171
Two-hybrid Homo sapiens
33 MTOR 2475
Affinity Capture-Western Homo sapiens
34 RGS11  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
FRET Homo sapiens
35 COPS5 10987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LDLR 3949
Positive Genetic Homo sapiens
37 RPA1 6117
Two-hybrid Homo sapiens
38 RGS9  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 GNG7 2788
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
40 GNG5 2787
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
41 PFDN5 5204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CUL4A 8451
Affinity Capture-Western Homo sapiens
43 DRD2  
Co-localization Homo sapiens
PCA Homo sapiens
44 ASPM 259266
Negative Genetic Homo sapiens
View the network image/svg+xml
 Pathways in which GNB5 is involved
PathwayEvidenceSource
Activation of G protein gated Potassium channels TAS Reactome
Activation of GABAB receptors TAS Reactome
Activation of kainate receptors upon glutamate binding TAS Reactome
ADORA2B mediated anti-inflammatory cytokines production TAS Reactome
ADP signalling through P2Y purinoceptor 1 TAS Reactome
ADP signalling through P2Y purinoceptor 1 IEA Reactome
ADP signalling through P2Y purinoceptor 12 TAS Reactome
Adrenaline,noradrenaline inhibits insulin secretion TAS Reactome
Adrenaline,noradrenaline inhibits insulin secretion IEA Reactome
Anti-inflammatory response favouring Leishmania parasite infection TAS Reactome
Aquaporin-mediated transport TAS Reactome
Beta-catenin independent WNT signaling TAS Reactome
Beta-catenin independent WNT signaling IEA Reactome
Ca2+ pathway TAS Reactome
Ca2+ pathway IEA Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
Chaperonin-mediated protein folding TAS Reactome
Class B/2 (Secretin family receptors) TAS Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding TAS Reactome
Disease TAS Reactome
ESR-mediated signaling TAS Reactome
Extra-nuclear estrogen signaling TAS Reactome
G alpha (12/13) signalling events TAS Reactome
G alpha (i) signalling events TAS Reactome
G alpha (q) signalling events TAS Reactome
G alpha (s) signalling events TAS Reactome
G alpha (z) signalling events TAS Reactome
G beta:gamma signalling through BTK TAS Reactome
G beta:gamma signalling through CDC42 TAS Reactome
G beta:gamma signalling through PI3Kgamma TAS Reactome
G beta:gamma signalling through PLC beta TAS Reactome
G protein gated Potassium channels TAS Reactome
G-protein activation TAS Reactome
G-protein beta:gamma signalling TAS Reactome
GABA B receptor activation TAS Reactome
GABA receptor activation TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Glucagon-type ligand receptors TAS Reactome
GPCR downstream signalling TAS Reactome
GPCR ligand binding TAS Reactome
GPER1 signaling TAS Reactome
Hemostasis TAS Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells TAS Reactome
Inactivation, recovery and regulation of the phototransduction cascade TAS Reactome
Inactivation, recovery and regulation of the phototransduction cascade IEA Reactome
Infectious disease TAS Reactome
Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits TAS Reactome
Integration of energy metabolism TAS Reactome
Integration of energy metabolism IEA Reactome
Inwardly rectifying K+ channels TAS Reactome
Leishmania infection TAS Reactome
Leishmania parasite growth and survival TAS Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of proteins TAS Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Opioid Signalling TAS Reactome
Parasitic Infection Pathways TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet homeostasis IEA Reactome
Platelet homeostasis TAS Reactome
Potassium Channels TAS Reactome
Presynaptic function of Kainate receptors TAS Reactome
Prostacyclin signalling through prostacyclin receptor IEA Reactome
Prostacyclin signalling through prostacyclin receptor TAS Reactome
Protein folding TAS Reactome
Regulation of insulin secretion TAS Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress TAS Reactome
Sensory Perception TAS Reactome
Sensory Perception IEA Reactome
Signal amplification TAS Reactome
Signal amplification IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
The phototransduction cascade TAS Reactome
The phototransduction cascade IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) TAS Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Thromboxane signalling through TP receptor TAS Reactome
Transmission across Chemical Synapses TAS Reactome
Transport of small molecules TAS Reactome
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome
Visual phototransduction TAS Reactome
Visual phototransduction IEA Reactome





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