Gene description for GNB3
Gene name guanine nucleotide binding protein (G protein), beta polypeptide 3
Gene symbol GNB3
Other names/aliases -
Species Homo sapiens
 Database cross references - GNB3
ExoCarta ExoCarta_2784
Vesiclepedia VP_2784
Entrez Gene 2784
HGNC 4400
MIM 139130
UniProt P16520  
 GNB3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for GNB3
Molecular Function
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    signaling receptor complex adaptor activity GO:0030159 IBA
    spectrin binding GO:0030507 IEA
    GTPase binding GO:0051020 IPI
Biological Process
    cell volume homeostasis GO:0006884 IGI
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    regulation of blood pressure GO:0008217 TAS
    regulation of gene expression GO:0010468 IGI
    regulation of glucose metabolic process GO:0010906 IGI
    regulation of hormone metabolic process GO:0032350 IGI
    regulation of fat cell differentiation GO:0045598 IGI
    regulation of feeding behavior GO:0060259 IGI
    regulation of cholesterol metabolic process GO:0090181 IGI
    regulation of triglyceride metabolic process GO:0090207 IGI
    regulation of locomotion involved in locomotory behavior GO:0090325 IGI
    regulation of phospholipid metabolic process GO:1903725 IGI
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    heterotrimeric G-protein complex GO:0005834 IBA
    plasma membrane GO:0005886 TAS
    dendrite GO:0030425 IEA
    cell body GO:0044297 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GNB3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 GNG10 2790
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
4 GNG2 54331
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
5 GNA12 2768
Affinity Capture-Western Homo sapiens
6 KIF14 9928
Affinity Capture-MS Homo sapiens
7 ZNF511  
Affinity Capture-MS Homo sapiens
8 GNG3 2785
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 ECT2 1894
Affinity Capture-MS Homo sapiens
10 PDCL 5082
Affinity Capture-Western Homo sapiens
11 GNG4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 CXCL6  
Affinity Capture-MS Homo sapiens
13 RLIM 51132
Affinity Capture-MS Homo sapiens
14 GNG8  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
15 CD47 961
Affinity Capture-Western Homo sapiens
16 KIF20A 10112
Affinity Capture-MS Homo sapiens
17 GNGT2  
Affinity Capture-Western Homo sapiens
18 GNB3 2784
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 GNGT1  
Affinity Capture-MS Homo sapiens
20 GNG13  
Affinity Capture-Western Homo sapiens
21 GNAQ 2776
Affinity Capture-Western Homo sapiens
22 RGS6 9628
Affinity Capture-Western Homo sapiens
23 GNG12 55970
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
24 ESRRB  
Affinity Capture-MS Homo sapiens
25 GNG11 2791
Affinity Capture-Western Homo sapiens
26 RASD2  
Two-hybrid Homo sapiens
27 MTOR 2475
Affinity Capture-Western Homo sapiens
28 ZNF131  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SLC36A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 GNB1 2782
Affinity Capture-MS Homo sapiens
31 RCAN2  
Affinity Capture-MS Homo sapiens
32 CHMP4B 128866
Affinity Capture-MS Homo sapiens
33 GNAI3 2773
Affinity Capture-Western Homo sapiens
34 GNAI2 2771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CIDEB  
Two-hybrid Homo sapiens
36 GNG7 2788
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
37 GNG5 2787
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
38 CXCR5  
Affinity Capture-Western Homo sapiens
39 CUL4A 8451
Affinity Capture-Western Homo sapiens
40 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GNB3 is involved
PathwayEvidenceSource
Activation of G protein gated Potassium channels TAS Reactome
Activation of GABAB receptors TAS Reactome
Activation of kainate receptors upon glutamate binding TAS Reactome
ADORA2B mediated anti-inflammatory cytokines production TAS Reactome
ADP signalling through P2Y purinoceptor 1 TAS Reactome
ADP signalling through P2Y purinoceptor 1 IEA Reactome
ADP signalling through P2Y purinoceptor 12 TAS Reactome
Adrenaline,noradrenaline inhibits insulin secretion TAS Reactome
Adrenaline,noradrenaline inhibits insulin secretion IEA Reactome
Anti-inflammatory response favouring Leishmania parasite infection TAS Reactome
Aquaporin-mediated transport TAS Reactome
Beta-catenin independent WNT signaling TAS Reactome
Beta-catenin independent WNT signaling IEA Reactome
Ca2+ pathway TAS Reactome
Ca2+ pathway IEA Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
Chaperonin-mediated protein folding TAS Reactome
Class B/2 (Secretin family receptors) TAS Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding TAS Reactome
Disease TAS Reactome
ESR-mediated signaling TAS Reactome
Extra-nuclear estrogen signaling TAS Reactome
G alpha (12/13) signalling events TAS Reactome
G alpha (i) signalling events TAS Reactome
G alpha (q) signalling events TAS Reactome
G alpha (s) signalling events TAS Reactome
G alpha (z) signalling events TAS Reactome
G beta:gamma signalling through BTK TAS Reactome
G beta:gamma signalling through CDC42 TAS Reactome
G beta:gamma signalling through PI3Kgamma TAS Reactome
G beta:gamma signalling through PLC beta TAS Reactome
G protein gated Potassium channels TAS Reactome
G-protein activation TAS Reactome
G-protein beta:gamma signalling TAS Reactome
GABA B receptor activation TAS Reactome
GABA receptor activation TAS Reactome
Glucagon signaling in metabolic regulation TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Glucagon-type ligand receptors TAS Reactome
GPCR downstream signalling TAS Reactome
GPCR ligand binding TAS Reactome
GPER1 signaling TAS Reactome
Hemostasis TAS Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells TAS Reactome
Incretin synthesis, secretion, and inactivation TAS Reactome
Infectious disease TAS Reactome
Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits TAS Reactome
Integration of energy metabolism TAS Reactome
Integration of energy metabolism IEA Reactome
Inwardly rectifying K+ channels TAS Reactome
Leishmania infection TAS Reactome
Leishmania parasite growth and survival TAS Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of proteins TAS Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Opioid Signalling TAS Reactome
Parasitic Infection Pathways TAS Reactome
Peptide hormone metabolism TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet homeostasis IEA Reactome
Platelet homeostasis TAS Reactome
Potassium Channels TAS Reactome
Presynaptic function of Kainate receptors TAS Reactome
Prostacyclin signalling through prostacyclin receptor IEA Reactome
Prostacyclin signalling through prostacyclin receptor TAS Reactome
Protein folding TAS Reactome
Regulation of insulin secretion TAS Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress TAS Reactome
Sensory Perception IEA Reactome
Sensory Perception TAS Reactome
Sensory perception of sweet, bitter, and umami (glutamate) taste IEA Reactome
Sensory perception of sweet, bitter, and umami (glutamate) taste TAS Reactome
Sensory perception of taste IEA Reactome
Sensory perception of taste TAS Reactome
Signal amplification TAS Reactome
Signal amplification IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) TAS Reactome
Thrombin signalling through proteinase activated receptors (PARs) TAS Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Thromboxane signalling through TP receptor TAS Reactome
Transmission across Chemical Synapses TAS Reactome
Transport of small molecules TAS Reactome
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here