Gene ontology annotations for L1CAM
Experiment description of studies that identified L1CAM in exosomes
1
Experiment ID
28
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSC70
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
19109410
Organism
Homo sapiens
Experiment description
Proteomic and immunologic analyses of brain tumor exosomes.
Authors
Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name
FASEB
Publication year
2008
Sample
Glioma cells
Sample name
X43,T D456MG H2159MG D54MG D247MG
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
2
Experiment ID
131
ISEV standards
✘
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
EpCAM|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
19188015
Organism
Homo sapiens
Experiment description
Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors
Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name
CL
Publication year
2009
Sample
Malignant ascites
Sample name
Malignant ascites - Ovarian cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.08-1.14 mg/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Cytofluorographic analysis Annexin-V-FITC Western blotting FACS
3
Experiment ID
131
ISEV standards
✘
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
EpCAM|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
19188015
Organism
Homo sapiens
Experiment description
Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors
Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name
CL
Publication year
2009
Sample
Malignant ascites
Sample name
Malignant ascites - Ovarian cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.08-1.14 mg/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Cytofluorographic analysis Annexin-V-FITC Western blotting FACS
4
Experiment ID
27
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
19109410
Organism
Homo sapiens
Experiment description
Proteomic and immunologic analyses of brain tumor exosomes.
Authors
Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name
FASEB
Publication year
2008
Sample
Medulloblastoma cells
Sample name
D283MED
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
5
Experiment ID
259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
A375M
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
6
Experiment ID
260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
1205Lu
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
7
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for L1CAM
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ANK2
Invivo
Homo sapiens
2
ITGA5
3678
Reconstituted Complex
Homo sapiens
3
EZR
7430
Reconstituted Complex
Homo sapiens
4
ITGAV
3685
Reconstituted Complex
Homo sapiens
5
AP2A1
160
Affinity Capture-Western
Homo sapiens
6
NCAM1
4684
Invitro
Homo sapiens
7
ANK1
286
Invivo
Homo sapiens
8
NUMB
8650
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
9
PEA15
Two-hybrid
Homo sapiens
10
CSPG3
Reconstituted Complex
Homo sapiens
11
CNTN2
Invitro
Homo sapiens
12
CNTN1
1272
Invivo
Homo sapiens
View the network
image/svg+xml
Pathways in which L1CAM is involved