Gene description for L1CAM
Gene name L1 cell adhesion molecule
Gene symbol L1CAM
Other names/aliases CAML1
CD171
HSAS
HSAS1
MASA
MIC5
N-CAM-L1
N-CAML1
NCAM-L1
S10
SPG1
Species Homo sapiens
 Database cross references - L1CAM
ExoCarta ExoCarta_3897
Vesiclepedia VP_3897
Entrez Gene 3897
HGNC 6470
MIM 308840
UniProt P32004  
 L1CAM identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Glioma cells 19109410    
Malignant ascites 19188015    
Medulloblastoma cells 19109410    
Melanoma cells 25950383    
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for L1CAM
Molecular Function
    protein binding GO:0005515 IPI
    axon guidance receptor activity GO:0008046 IBA
    protein domain specific binding GO:0019904 IDA
Biological Process
    chemotaxis GO:0006935 TAS
    cell adhesion GO:0007155 NAS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IBA
    cell-matrix adhesion GO:0007160 IDA
    nervous system development GO:0007399 TAS
    axon guidance GO:0007411 IDA
    cell migration GO:0016477 IDA
    neuron projection development GO:0031175 IDA
    positive regulation of axon extension GO:0045773 ISS
    synapse organization GO:0050808 IBA
    synapse organization GO:0050808 IDA
    axon development GO:0061564 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    cell surface GO:0009986 HDA
    cell surface GO:0009986 IDA
    axon GO:0030424 IBA
    axon GO:0030424 IDA
    dendrite GO:0030425 IEA
    neuronal cell body GO:0043025 IBA
    neuronal cell body GO:0043025 IDA
    axonal growth cone GO:0044295 ISS
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified L1CAM in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 28
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Homo sapiens
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Glioma cells
Sample name "X43,T
D456MG
H2159MG
D54MG
D247MG"
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 131
MISEV standards
✘ Biophysical techniques
✔
HSP70|EpCAM|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors "Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P."
Journal name CL
Publication year 2009
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
Western blotting
FACS
8
Experiment ID 131
MISEV standards
✘ Biophysical techniques
✔
HSP70|EpCAM|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors "Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P."
Journal name CL
Publication year 2009
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
Western blotting
FACS
9
Experiment ID 27
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Homo sapiens
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Medulloblastoma cells
Sample name D283MED
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for L1CAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GPM6A 2823
Affinity Capture-MS Homo sapiens
2 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CTSE  
Affinity Capture-MS Homo sapiens
4 BTNL2  
Affinity Capture-MS Homo sapiens
5 ERBB2 2064
Affinity Capture-Western Homo sapiens
6 FGFR1 2260
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
7 CANX 821
Affinity Capture-Western Homo sapiens
8 PRNP 5621
Affinity Capture-MS Homo sapiens
9 LINC00839  
Protein-RNA Homo sapiens
10 RABGEF1 27342
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 EZR 7430
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 LGALS1 3956
Affinity Capture-MS Homo sapiens
13 CBLN4  
Affinity Capture-MS Homo sapiens
14 EGFR 1956
Affinity Capture-Western Homo sapiens
15 FBXO6 26270
Affinity Capture-MS Homo sapiens
16 ERBB3 2065
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
17 NRP1 8829
Reconstituted Complex Homo sapiens
18 ITGA5 3678
Reconstituted Complex Homo sapiens
19 KIAA1429 25962
Affinity Capture-MS Homo sapiens
20 ITGAV 3685
Reconstituted Complex Homo sapiens
21 RANBP9 10048
Two-hybrid Homo sapiens
22 NUMB 8650
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 FBXO2 26232
Affinity Capture-MS Homo sapiens
24 NTRK1 4914
Affinity Capture-MS Homo sapiens
25 Caskin1  
Two-hybrid Rattus norvegicus
26 AP2A1 160
Affinity Capture-Western Homo sapiens
27 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NCAN  
Reconstituted Complex Homo sapiens
31 PTCH1  
Affinity Capture-MS Homo sapiens
32 PEA15 8682
Two-hybrid Homo sapiens
33 CNTN2  
Reconstituted Complex Homo sapiens
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