Gene description for SLC12A5
Gene name solute carrier family 12 (potassium/chloride transporter), member 5
Gene symbol SLC12A5
Other names/aliases KCC2
Species Homo sapiens
 Database cross references - SLC12A5
ExoCarta ExoCarta_57468
Entrez Gene 57468
HGNC 13818
MIM 606726
UniProt Q9H2X9  
 SLC12A5 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC12A5
Molecular Function
    potassium:chloride symporter activity GO:0015379 NAS
    protein kinase binding GO:0019901 IPI
Biological Process
    ion transport GO:0006811 TAS
    potassium ion transport GO:0006813 IEA
    multicellular organism growth GO:0035264 IEA
    cellular ion homeostasis GO:0006873 NAS
    chloride transmembrane transport GO:1902476 IEA
    transmembrane transport GO:0055085 TAS
    learning GO:0007612 IEA
    thermosensory behavior GO:0040040 IEA
    response to drug GO:0042493 IEA
    transport GO:0006810 NAS
    synaptic transmission GO:0007268 IEA
Subcellular Localization
    neuronal cell body GO:0043025 IEA
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 NAS
    integral component of plasma membrane GO:0005887 IEA
    dendritic shaft GO:0043198 IEA
 Experiment description of studies that identified SLC12A5 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC12A5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SLC12A5 is involved
PathwayEvidenceSource
Cation-coupled Chloride cotransporters TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here