Experiment description of studies that identified SDCBP2 in exosomes

1 Experiment ID 76 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI Enriched markers ✔ HSP90B1 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20224111     Organism Homo sapiens Experiment description Proteomics analysis of bladder cancer exosomes. Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A." Journal name MCP Publication year 2010 Sample Bladder cancer cells Sample name HT1376 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.10-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF/TOF] Western blotting FACS

2 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

3 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 282 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

7 Experiment ID 283 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

8 Experiment ID 285 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

9 Experiment ID 286 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

10 Experiment ID 1203 MISEV standards ✔ EM Biophysical techniques ✔ SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

11 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for SDCBP2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CT45A1   Two-hybrid Homo sapiens 2 EIF1AD 84285 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 3 PRM2   Two-hybrid Homo sapiens 4 CT45A10   Two-hybrid Homo sapiens 5 SEC23A 10484 Two-hybrid Homo sapiens 6 NUF2   Two-hybrid Homo sapiens 7 LGALS2   Two-hybrid Homo sapiens 8 TIFA   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 9 PTMS 5763 Two-hybrid Homo sapiens 10 LGALS14   Two-hybrid Homo sapiens 11 C11orf57   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 12 PAFAH1B3 5050 Two-hybrid Homo sapiens 13 CHCHD1   Two-hybrid Homo sapiens 14 AKAP17A   Two-hybrid Homo sapiens 15 SDCBP2 27111 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 16 ZCCHC10   Two-hybrid Homo sapiens 17 GABRA3   Two-hybrid Homo sapiens 18 TDO2   Two-hybrid Homo sapiens 19 ADAP1   Two-hybrid Homo sapiens 20 FAM9B   Two-hybrid Homo sapiens 21 CT45A3 441519 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 22 NOP56 10528 Two-hybrid Homo sapiens 23 TCP11L1 55346 Two-hybrid Homo sapiens 24 DPPA2   Two-hybrid Homo sapiens 25 FAM13C   Two-hybrid Homo sapiens 26 NFU1 27247 Two-hybrid Homo sapiens 27 CDK15   Affinity Capture-MS Homo sapiens 28 C1orf35   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 29 THAP6   Two-hybrid Homo sapiens 30 PRPF40A 55660 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 31 ARF4 378 Two-hybrid Homo sapiens 32 TNP1   Two-hybrid Homo sapiens 33 PRR13   Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 34 YTHDC1   Two-hybrid Homo sapiens 35 SYT17   Two-hybrid Homo sapiens 36 NPM2   Two-hybrid Homo sapiens 37 RPL41   Two-hybrid Homo sapiens 38 ZCCHC17   Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 39 RNPS1 10921 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 40 SREK1IP1   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 41 PRPF38A 84950 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 42 FAM83A   Affinity Capture-MS Homo sapiens 43 PHC1   Two-hybrid Homo sapiens 44 HSBP1 3281 Two-hybrid Homo sapiens 45 RNF111 54778 Two-hybrid Homo sapiens 46 BEND7 222389 Two-hybrid Homo sapiens 47 RPL22 6146 Two-hybrid Homo sapiens 48 CRY2   Two-hybrid Homo sapiens 49 LYAR 55646 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 50 FBXO28   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 51 ZNF581   Two-hybrid Homo sapiens 52 ZRSR2   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 53 VAX1   Two-hybrid Homo sapiens 54 CTH 1491 Two-hybrid Homo sapiens 55 RPL22L1 200916 Two-hybrid Homo sapiens 56 CRCT1   Two-hybrid Homo sapiens 57 FAM133A 286499 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 58 CT45A5   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 59 VPS25 84313 Two-hybrid Homo sapiens 60 TNNT1   Two-hybrid Homo sapiens View the network

Pathways in which SDCBP2 is involved

No pathways found