ExoCarta: Gene summary

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Gene description for HSBP1
Gene name	heat shock factor binding protein 1
Gene symbol	HSBP1
Other names/aliases	NPC-A-13
Species	Homo sapiens
Database cross references - HSBP1
ExoCarta	ExoCarta_3281
Vesiclepedia	VP_3281
Entrez Gene	3281
HGNC	5203
MIM	604553
UniProt	O75506
HSBP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Hepatocellular carcinoma cells	26054723

Gene ontology annotations for HSBP1

Molecular Function
	transcription corepressor activity	GO:0003714	IEA
	protein binding	GO:0005515	IPI
	identical protein binding	GO:0042802	IPI
Biological Process
	negative regulation of transcription by RNA polymerase II	GO:0000122	TAS
	axonal transport of mitochondrion	GO:0019896	IMP
	endodermal cell differentiation	GO:0035987	IEA
	cellular heat acclimation	GO:0070370	IBA
Subcellular Localization
	nucleus	GO:0005634	IBA
	nucleoplasm	GO:0005654	TAS
	cytosol	GO:0005829	IBA
	axon cytoplasm	GO:1904115	IEA

Experiment description of studies that identified HSBP1 in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

236

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|HSC70\|GAPDH	Enriched markers
✔ HSP90B1	Negative markers
✔ qNano	Particle analysis

Identified molecule

mRNA

Identification method

RNA Sequencing

PubMed ID

26054723

Organism

Homo sapiens

Experiment description

Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs

Authors

"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"

Journal name

Carcinogenesis

Publication year

2015

Sample

Hepatocellular carcinoma cells

Sample name

MHCC97L

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.19 g/mL

Molecules identified in the study

Protein
RNA

Methods used in the study

Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing

Protein-protein interactions for HSBP1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

KRTAP10-7

Two-hybrid

Homo sapiens

GSX1

Affinity Capture-MS

Homo sapiens

RNF175

Affinity Capture-MS

Homo sapiens

IFT57

55081

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NUF2

Affinity Capture-MS

Homo sapiens

SDCBP

6386

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

RRBP1

6238

Co-fractionation

Homo sapiens

BLOC1S6

Affinity Capture-MS

Homo sapiens

FOXA1

Affinity Capture-MS

Homo sapiens

EEA1

8411

Affinity Capture-MS

Homo sapiens

SDCBP2

27111

Two-hybrid

Homo sapiens

CCHCR1

Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens

KIFC3

3801

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

WASF2

10163

Affinity Capture-MS

Homo sapiens

NOTCH2NL

388677

Two-hybrid

Homo sapiens

KIAA1217

56243

Two-hybrid

Homo sapiens

TMCC3

Affinity Capture-MS

Homo sapiens

MIF

4282

Affinity Capture-MS

Homo sapiens

BSPRY

54836

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HSBP1

3281

Reconstituted Complex		Homo sapiens
Co-crystal Structure		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Reconstituted Complex		Homo sapiens
Co-crystal Structure		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

ATG101

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KRTAP1-1

Two-hybrid

Homo sapiens

RB1CC1

9821

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FAM9C

Affinity Capture-MS

Homo sapiens

KRTAP10-3

Two-hybrid

Homo sapiens

STX11

8676

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

BRK1

55845

Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens

HSF2BP

Affinity Capture-MS

Homo sapiens

CAPZB

832

Affinity Capture-MS

Homo sapiens

FARSB

10056

Affinity Capture-MS

Homo sapiens

BLOC1S5

63915

Two-hybrid

Homo sapiens

S100A4

6275

Affinity Capture-MS

Homo sapiens

DYRK1A

1859

Affinity Capture-MS

Homo sapiens

CYSRT1

375791

Two-hybrid

Homo sapiens

HSF1

Two-hybrid		Homo sapiens
Affinity Capture-Western		Homo sapiens

FAM24B

Affinity Capture-MS

Homo sapiens

GORASP2

26003

Two-hybrid

Homo sapiens

HSPA4

3308

Affinity Capture-Western

Homo sapiens

RCAN2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

BEND3

Affinity Capture-MS

Homo sapiens

CCDC53

51019

Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

POC1B

282809

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MPP7

143098

Two-hybrid

Homo sapiens

WASH1

100287171

Two-hybrid

Homo sapiens

COG7

91949

Affinity Capture-MS

Homo sapiens

LURAP1

Affinity Capture-MS

Homo sapiens

LNX1

Two-hybrid

Homo sapiens

C17orf59

54785

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KRTAP10-9

Two-hybrid

Homo sapiens

VCP

7415

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which HSBP1 is involved

Pathway	Evidence	Source
Attenuation phase	TAS	Reactome
Cellular response to heat stress	TAS	Reactome
Cellular responses to stimuli	TAS	Reactome
Cellular responses to stress	TAS	Reactome
HSF1 activation	TAS	Reactome
HSF1-dependent transactivation	TAS	Reactome

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