Gene description for EPB42
Gene name erythrocyte membrane protein band 4.2
Gene symbol EPB42
Other names/aliases PA
SPH5
Species Homo sapiens
 Database cross references - EPB42
ExoCarta ExoCarta_2038
Entrez Gene 2038
HGNC 3381
MIM 177070
UniProt P16452  
 EPB42 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for EPB42
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of cytoskeleton GO:0005200 TAS
    ATP binding GO:0005524 TAS
    protein-glutamine gamma-glutamyltransferase activity GO:0003810 IEA
Biological Process
    regulation of cell shape GO:0008360 IEA
    hemoglobin metabolic process GO:0020027 IEA
    iron ion homeostasis GO:0055072 IEA
    peptide cross-linking GO:0018149 IEA
    cell morphogenesis GO:0000902 IEA
    erythrocyte maturation GO:0043249 IEA
    spleen development GO:0048536 IEA
Subcellular Localization
    cortical cytoskeleton GO:0030863 IEA
    plasma membrane GO:0005886 IEA
    cytoskeleton GO:0005856 TAS
 Experiment description of studies that identified EPB42 in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EPB42
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC4A2 6522
Invitro Homo sapiens
Invitro Homo sapiens
2 KRAS 3845
Invitro Homo sapiens
3 ECG2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 SPTAN1 6709
Invitro Homo sapiens
5 EPB49  
Invivo Homo sapiens
6 EPB41 2035
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which EPB42 is involved
No pathways found





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