Gene description for EPB42
Gene name erythrocyte membrane protein band 4.2
Gene symbol EPB42
Other names/aliases PA
SPH5
Species Homo sapiens
 Database cross references - EPB42
ExoCarta ExoCarta_2038
Vesiclepedia VP_2038
Entrez Gene 2038
HGNC 3381
MIM 177070
UniProt P16452  
 EPB42 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for EPB42
Molecular Function
    protein-glutamine gamma-glutamyltransferase activity GO:0003810 IBA
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 TAS
Biological Process
    cell morphogenesis GO:0000902 IEA
    cytoskeleton organization GO:0007010 IEA
    regulation of cell shape GO:0008360 IEA
    hemoglobin metabolic process GO:0020027 IEA
    erythrocyte maturation GO:0043249 IEA
    spleen development GO:0048536 IEA
    multicellular organismal-level iron ion homeostasis GO:0060586 IEA
Subcellular Localization
    cytoskeleton GO:0005856 TAS
    plasma membrane GO:0005886 IEA
    cortical cytoskeleton GO:0030863 IEA
    ankyrin-1 complex GO:0170014 IDA
 Experiment description of studies that identified EPB42 in sEVs
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EPB42
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC4A7 9497
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SLC4A1 6521
Protein-peptide Homo sapiens
3 KRAS 3845
Reconstituted Complex Homo sapiens
4 MAPK14 1432
Two-hybrid Homo sapiens
5 SPTAN1 6709
Reconstituted Complex Homo sapiens
6 ANK2 287
Reconstituted Complex Homo sapiens
7 SPINK7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SNX6 58533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EPB42 is involved
No pathways found





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