Gene description for SLC4A1
Gene name solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
Gene symbol SLC4A1
Other names/aliases AE1
BND3
CD233
DI
EMPB3
EPB3
FR
RTA1A
SW
WD
WD1
WR
Species Homo sapiens
 Database cross references - SLC4A1
ExoCarta ExoCarta_6521
Vesiclepedia VP_6521
Entrez Gene 6521
HGNC 11027
MIM 109270
UniProt P02730  
 SLC4A1 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for SLC4A1
Molecular Function
    anion transmembrane transporter activity GO:0008509 TAS
    protein anchor GO:0043495 TAS
    anion:anion antiporter activity GO:0015301 IDA
    actin binding GO:0003779 IEA
    chloride transmembrane transporter activity GO:0015108 ISS
    protein homodimerization activity GO:0042803 IPI
    protein binding GO:0005515 IPI
    ankyrin binding GO:0030506 IPI
    protein C-terminus binding GO:0008022 IEA
    bicarbonate transmembrane transporter activity GO:0015106 IDA
    inorganic anion exchanger activity GO:0005452 TAS
Biological Process
    chloride transmembrane transport GO:1902476 IBA
    ion transport GO:0006811 TAS
    transmembrane transport GO:0055085 TAS
    small molecule metabolic process GO:0044281 TAS
    anion transport GO:0006820 IDA
    regulation of intracellular pH GO:0051453 IBA
    chloride transport GO:0006821 ISS
    cellular ion homeostasis GO:0006873 TAS
    bicarbonate transport GO:0015701 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 NAS
    extracellular exosome GO:0070062 IDA
    cortical cytoskeleton GO:0030863 IDA
    Z disc GO:0030018 ISS
    integral component of plasma membrane GO:0005887 IDA
    blood microparticle GO:0072562 IDA
    basolateral plasma membrane GO:0016323 IDA
 Experiment description of studies that identified SLC4A1 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SLC4A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC4A1 6521
Invitro Homo sapiens
Co-crystal Structure Homo sapiens
Invitro Homo sapiens
Co-crystal Structure Homo sapiens
2 SLC4A1AP  
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 ANK1 286
Invitro Homo sapiens
4 RHAG  
Invivo Homo sapiens
5 CA2 760
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
6 CANX 821
Invivo Homo sapiens
Invitro Homo sapiens
7 CA4 762
Invitro Homo sapiens
8 EPB41 2035
Invitro Homo sapiens
View the network image/svg+xml



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