Gene description for Uchl1
Gene name ubiquitin carboxy-terminal hydrolase L1
Gene symbol Uchl1
Other names/aliases AW822034
C88048
PGP 9.5
PGP9.5
R75593
UCH-L1
UCHL-1
gad
Species Mus musculus
 Database cross references - Uchl1
ExoCarta ExoCarta_22223
Vesiclepedia VP_22223
Entrez Gene 22223
UniProt Q9R0P9  
 Uchl1 identified in exosomes derived from the following tissue/cell type
Pancreatic cells 19351151    
 Gene ontology annotations for Uchl1
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 ISO
    cysteine-type endopeptidase activity GO:0004197 ISS
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    omega peptidase activity GO:0008242 ISO
    omega peptidase activity GO:0008242 ISS
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    alpha-2A adrenergic receptor binding GO:0031694 IEA
    alpha-2A adrenergic receptor binding GO:0031694 ISO
    ribosome binding GO:0043022 IDA
    ubiquitin binding GO:0043130 IDA
    ubiquitin binding GO:0043130 ISO
    ubiquitin binding GO:0043130 ISS
Biological Process
    male germ cell proliferation GO:0002176 IMP
    response to ischemia GO:0002931 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    axonogenesis GO:0007409 IMP
    axon target recognition GO:0007412 IMP
    adult walking behavior GO:0007628 IGI
    adult walking behavior GO:0007628 IMP
    protein deubiquitination GO:0016579 ISO
    protein deubiquitination GO:0016579 ISS
    axonal transport of mitochondrion GO:0019896 IMP
    protein catabolic process GO:0030163 IBA
    eating behavior GO:0042755 IGI
    negative regulation of MAP kinase activity GO:0043407 ISO
    positive regulation of glycolytic process GO:0045821 IEA
    positive regulation of glycolytic process GO:0045821 ISO
    neuromuscular process GO:0050905 IMP
    muscle cell development GO:0055001 IGI
    cellular response to xenobiotic stimulus GO:0071466 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    axon GO:0030424 IDA
    neuronal cell body GO:0043025 IDA
    myelin sheath GO:0043209 HDA
    neuron projection terminus GO:0044306 IDA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Uchl1 in exosomes
1
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Uchl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cdkn1b  
Co-fractionation Mus musculus
2 Ptov1  
Affinity Capture-Western Mus musculus
3 Cdk1 12534
Affinity Capture-Western Mus musculus
4 Cops5 26754
Affinity Capture-Western Mus musculus
5 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
Co-purification Mus musculus
6 Ncam1 17967
Co-fractionation Mus musculus
7 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 MAPT  
Reconstituted Complex Homo sapiens
10 Mapt  
Affinity Capture-MS Mus musculus
11 MTNR1A  
Reconstituted Complex Homo sapiens
12 Tuba1a 22142
Affinity Capture-Western Mus musculus
13 Cd36 12491
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
14 Lamp2 16784
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
15 Park2  
Reconstituted Complex Mus musculus
16 Sharpin  
Affinity Capture-MS Mus musculus
17 Nefl  
Co-fractionation Mus musculus
18 Uba1 22201
Co-fractionation Mus musculus
19 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Uchl1 is involved
PathwayEvidenceSource
Deubiquitination IEA Reactome
Metabolism of proteins IEA Reactome
Post-translational protein modification IEA Reactome
UCH proteinases IEA Reactome





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