Gene description for Ncam1
Gene name neural cell adhesion molecule 1
Gene symbol Ncam1
Other names/aliases CD56
E-NCAM
NCAM-1
Ncam
Species Mus musculus
 Database cross references - Ncam1
ExoCarta ExoCarta_17967
Vesiclepedia VP_17967
Entrez Gene 17967
UniProt P13595  
 Ncam1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ncam1
Molecular Function
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IEA
    phosphatase binding GO:0019902 ISO
    LRR domain binding GO:0030275 ISO
Biological Process
    epithelial to mesenchymal transition GO:0001837 IDA
    regulation of exocyst assembly GO:0001928 IMP
    regulation of protein phosphorylation GO:0001932 ISO
    cell surface receptor signaling pathway GO:0007166 IDA
    axonal fasciculation GO:0007413 ISO
    learning or memory GO:0007611 ISO
    response to xenobiotic stimulus GO:0009410 ISO
    response to lead ion GO:0010288 ISO
    calcium-mediated signaling GO:0019722 IDA
    thalamus development GO:0021794 ISO
    neuron projection development GO:0031175 IGI
    multicellular organismal response to stress GO:0033555 ISO
    homotypic cell-cell adhesion GO:0034109 IMP
    response to cocaine GO:0042220 ISO
    negative regulation of programmed cell death GO:0043069 ISO
    neuron development GO:0048666 ISO
    modulation of chemical synaptic transmission GO:0050804 ISO
    positive regulation of calcium-mediated signaling GO:0050850 IDA
    positive regulation of cardiac muscle cell proliferation GO:0060045 ISO
    cellular response to ethanol GO:0071361 ISO
    commissural neuron axon guidance GO:0071679 IGI
    regulation of semaphorin-plexin signaling pathway GO:2001260 IGI
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    external side of plasma membrane GO:0009897 IDA
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 ISO
    axon GO:0030424 IDA
    growth cone GO:0030426 IDA
    presynaptic membrane GO:0042734 ISO
    neuron projection GO:0043005 IBA
    neuronal cell body GO:0043025 IDA
    neuronal cell body GO:0043025 ISO
    myelin sheath GO:0043209 HDA
    postsynaptic membrane GO:0045211 ISO
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Ncam1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
4
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ncam1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Syngap1  
Affinity Capture-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
5 Prnp 19122
Affinity Capture-MS Mus musculus
6 Sptan1 20740
Affinity Capture-Western Mus musculus
7 Ywhab 54401
Affinity Capture-Western Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
9 Homer1  
Affinity Capture-MS Mus musculus
10 Ptk2  
Co-fractionation Mus musculus
11 Uchl1 22223
Co-fractionation Mus musculus
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