Gene description for B4GALT4
Gene name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
Gene symbol B4GALT4
Other names/aliases B4Gal-T4
beta4Gal-T4
Species Homo sapiens
 Database cross references - B4GALT4
ExoCarta ExoCarta_8702
Vesiclepedia VP_8702
Entrez Gene 8702
HGNC 927
MIM 604015
UniProt O60513  
 B4GALT4 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for B4GALT4
Molecular Function
    N-acetyllactosamine synthase activity GO:0003945 IDA
    protein binding GO:0005515 IPI
    galactosyltransferase activity GO:0008378 IBA
    UDP-galactosyltransferase activity GO:0035250 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    lactosylceramide biosynthetic process GO:0001572 IDA
    carbohydrate metabolic process GO:0005975 IEA
    protein glycosylation GO:0006486 IEA
    membrane lipid metabolic process GO:0006643 TAS
    keratan sulfate biosynthetic process GO:0018146 IDA
    keratan sulfate biosynthetic process GO:0018146 IMP
    glycosylation GO:0070085 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 TAS
 Experiment description of studies that identified B4GALT4 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for B4GALT4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 NGLY1 55768
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CCL3  
Affinity Capture-MS Homo sapiens
4 CBWD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HSPA5 3309
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HS3ST1  
Affinity Capture-MS Homo sapiens
7 Tubg1 103733
Affinity Capture-MS Mus musculus
8 Psmb5 19173
Affinity Capture-MS Mus musculus
9 CLDND1 56650
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SORT1 6272
Affinity Capture-MS Homo sapiens
11 CST8  
Affinity Capture-MS Homo sapiens
12 PDGFRA 5156
Affinity Capture-MS Homo sapiens
13 PRG2  
Affinity Capture-MS Homo sapiens
14 STRN3 29966
Affinity Capture-MS Homo sapiens
15 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
16 ITIH2 3698
Affinity Capture-MS Homo sapiens
17 ITGA6 3655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SLURP1  
Affinity Capture-MS Homo sapiens
19 DDX58 23586
Affinity Capture-RNA Homo sapiens
20 NXF1 10482
Affinity Capture-RNA Homo sapiens
21 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
22 MOV10 4343
Affinity Capture-RNA Homo sapiens
23 SDF2L1 23753
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CRLF1 9244
Affinity Capture-MS Homo sapiens
25 CGREF1 10669
Affinity Capture-MS Homo sapiens
26 SCGB2A2  
Affinity Capture-MS Homo sapiens
27 C1orf54  
Affinity Capture-MS Homo sapiens
28 CRP  
Affinity Capture-MS Homo sapiens
29 LRRC8E 80131
Affinity Capture-MS Homo sapiens
30 CLEC11A 6320
Affinity Capture-MS Homo sapiens
31 DNAJC3 5611
Affinity Capture-MS Homo sapiens
32 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
33 HSPG2 3339
Affinity Capture-MS Homo sapiens
34 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 TMPRSS13  
Affinity Capture-MS Homo sapiens
36 PIPSL 266971
Affinity Capture-MS Homo sapiens
37 SP3  
Affinity Capture-MS Homo sapiens
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