Gene description for CPS1
Gene name carbamoyl-phosphate synthase 1, mitochondrial
Gene symbol CPS1
Other names/aliases CPSASE1
PHN
Species Homo sapiens
 Database cross references - CPS1
ExoCarta ExoCarta_1373
Vesiclepedia VP_1373
Entrez Gene 1373
HGNC 2323
MIM 608307
UniProt P31327  
 CPS1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CPS1
Molecular Function
    carbamoyl-phosphate synthase (ammonia) activity GO:0004087 EXP
    carbamoyl-phosphate synthase (ammonia) activity GO:0004087 IBA
    carbamoyl-phosphate synthase (ammonia) activity GO:0004087 IMP
    carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity GO:0004088 IBA
    endopeptidase activity GO:0004175 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    phospholipid binding GO:0005543 IEA
    glutamate binding GO:0016595 IEA
    potassium ion binding GO:0030955 EXP
    small molecule binding GO:0036094 EXP
    protein-containing complex binding GO:0044877 IEA
    metal ion binding GO:0046872 EXP
    modified amino acid binding GO:0072341 IDA
Biological Process
    urea cycle GO:0000050 NAS
    urea cycle GO:0000050 TAS
    'de novo' pyrimidine nucleobase biosynthetic process GO:0006207 IEA
    glutamine metabolic process GO:0006541 IBA
    midgut development GO:0007494 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to toxic substance GO:0009636 IEA
    response to zinc ion GO:0010043 IEA
    response to amine GO:0014075 IEA
    citrulline biosynthetic process GO:0019240 NAS
    triglyceride catabolic process GO:0019433 IMP
    response to food GO:0032094 IEA
    response to lipopolysaccharide GO:0032496 IDA
    vasodilation GO:0042311 IMP
    response to starvation GO:0042594 IEA
    response to amino acid GO:0043200 IEA
    cellular response to fibroblast growth factor stimulus GO:0044344 IEA
    nitric oxide metabolic process GO:0046209 IMP
    homocysteine metabolic process GO:0050667 IDA
    monoatomic anion homeostasis GO:0055081 IEA
    response to growth hormone GO:0060416 IEA
    hepatocyte differentiation GO:0070365 IEA
    carbamoyl phosphate biosynthetic process GO:0070409 IMP
    cellular response to ammonium ion GO:0071242 IMP
    cellular response to cAMP GO:0071320 IEA
    cellular response to glucagon stimulus GO:0071377 IEA
    cellular response to oleic acid GO:0071400 IEA
    response to dexamethasone GO:0071548 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial matrix GO:0005759 TAS
    plasma membrane GO:0005886 IEA
    protein-containing complex GO:0032991 IEA
    mitochondrial nucleoid GO:0042645 IDA
 Experiment description of studies that identified CPS1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CPS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Co-fractionation Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 FOXF1 2294
Affinity Capture-MS Homo sapiens
4 RAMP3  
Affinity Capture-MS Homo sapiens
5 HNRNPH1 3187
Co-fractionation Homo sapiens
6 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
7 Cdk1 12534
Affinity Capture-MS Mus musculus
8 SPRTN  
Affinity Capture-MS Homo sapiens
9 KRT1 3848
Affinity Capture-MS Homo sapiens
10 Ruvbl1 56505
Affinity Capture-MS Mus musculus
11 Ormdl1  
Affinity Capture-MS Mus musculus
12 FBXL4 26235
Affinity Capture-MS Homo sapiens
13 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ALB 213
Affinity Capture-MS Homo sapiens
15 DEFB121  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MTG2  
Affinity Capture-MS Homo sapiens
17 KIF20A 10112
Affinity Capture-MS Homo sapiens
18 UBC 7316
Affinity Capture-MS Homo sapiens
19 BPNT1 10380
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 CPT1A 1374
Co-fractionation Homo sapiens
21 LRPPRC 10128
Co-fractionation Homo sapiens
22 KIAA1429 25962
Affinity Capture-MS Homo sapiens
23 LMO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 B3GNT2 10678
Affinity Capture-MS Homo sapiens
25 TRMU  
Affinity Capture-MS Homo sapiens
26 KIF23 9493
Affinity Capture-MS Homo sapiens
27 MRPL23 6150
Affinity Capture-MS Homo sapiens
28 FAM174A 345757
Affinity Capture-MS Homo sapiens
29 YARS2  
Affinity Capture-MS Homo sapiens
30 MRPS24 64951
Affinity Capture-MS Homo sapiens
31 GCAT  
Affinity Capture-MS Homo sapiens
32 HSPH1 10808
Co-fractionation Homo sapiens
33 FTSJ2 29960
Affinity Capture-MS Homo sapiens
34 PRC1 9055
Affinity Capture-MS Homo sapiens
35 MALSU1  
Affinity Capture-MS Homo sapiens
36 DNAJC7 7266
Affinity Capture-MS Homo sapiens
37 XRCC3  
Affinity Capture-MS Homo sapiens
38 NAA38  
Affinity Capture-MS Homo sapiens
39 KIF14 9928
Affinity Capture-MS Homo sapiens
40 HSPA8 3312
Co-fractionation Homo sapiens
41 AP1S2 8905
Affinity Capture-MS Homo sapiens
42 AGO2 27161
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CHMP4C 92421
Affinity Capture-MS Homo sapiens
44 MRPS25 64432
Affinity Capture-MS Homo sapiens
45 SLC25A10 1468
Affinity Capture-MS Homo sapiens
46 ACSM5 54988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 MRPS26 64949
Co-fractionation Homo sapiens
48 DHRS2 10202
Affinity Capture-MS Homo sapiens
49 TUBA1C 84790
Affinity Capture-MS Homo sapiens
50 THBS3 7059
Affinity Capture-MS Homo sapiens
51 CLEC11A 6320
Affinity Capture-MS Homo sapiens
52 YWHAQ 10971
Affinity Capture-MS Homo sapiens
53 Bag2  
Affinity Capture-MS Mus musculus
54 CHMP4B 128866
Affinity Capture-MS Homo sapiens
55 TMEM70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 RAD21 5885
Affinity Capture-Western Homo sapiens
57 ANLN 54443
Affinity Capture-MS Homo sapiens
58 HSPA5 3309
Affinity Capture-MS Homo sapiens
59 OTC 5009
Affinity Capture-MS Homo sapiens
60 RARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 MRPL21 219927
Affinity Capture-MS Homo sapiens
62 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
63 METTL14  
Affinity Capture-MS Homo sapiens
64 ECT2 1894
Affinity Capture-MS Homo sapiens
65 KIRREL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 C2orf47  
Affinity Capture-MS Homo sapiens
67 FOXG1  
Affinity Capture-MS Homo sapiens
68 RICTOR 253260
Affinity Capture-MS Homo sapiens
69 FAHD1 81889
Affinity Capture-MS Homo sapiens
70 HSD17B10 3028
Two-hybrid Homo sapiens
71 FBXO6 26270
Affinity Capture-MS Homo sapiens
72 TOMM70A 9868
Co-fractionation Homo sapiens
73 AURKB 9212
Affinity Capture-MS Homo sapiens
74 DMAP1 55929
Affinity Capture-MS Homo sapiens
75 ARMC6 93436
Affinity Capture-MS Homo sapiens
76 PCBP1 5093
Co-fractionation Homo sapiens
77 MYC  
Dosage Lethality Homo sapiens
78 FH 2271
Co-fractionation Homo sapiens
79 LDHAL6B 92483
Affinity Capture-MS Homo sapiens
80 HSPA1A 3303
Co-fractionation Homo sapiens
81 HSPA4 3308
Co-fractionation Homo sapiens
82 FOXRED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 Rpl35 66489
Affinity Capture-MS Mus musculus
84 Mad2l1 56150
Affinity Capture-MS Mus musculus
85 HSPA9 3313
Co-fractionation Homo sapiens
86 ARAF 369
Two-hybrid Homo sapiens
87 H2AFX 3014
Affinity Capture-MS Homo sapiens
88 Lgals3bp 19039
Affinity Capture-MS Mus musculus
89 AK4 205
Affinity Capture-MS Homo sapiens
90 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
91 Tubg1 103733
Affinity Capture-MS Mus musculus
92 MRPS2 51116
Affinity Capture-MS Homo sapiens
93 CIT 11113
Affinity Capture-MS Homo sapiens
94 TUBG1 7283
Affinity Capture-MS Homo sapiens
95 ASB1 51665
Affinity Capture-MS Homo sapiens
96 ACAA2 10449
Affinity Capture-MS Homo sapiens
97 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
99 FAM83F 113828
Affinity Capture-MS Homo sapiens
100 Csnk1e  
Affinity Capture-MS Mus musculus
101 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
102 TCF4  
Affinity Capture-MS Homo sapiens
103 RPUSD3  
Affinity Capture-MS Homo sapiens
104 MYL10 93408
Affinity Capture-MS Homo sapiens
105 OXCT2  
Affinity Capture-MS Homo sapiens
106 LMO1  
Affinity Capture-MS Homo sapiens
107 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 TRIM43  
Affinity Capture-MS Homo sapiens
109 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CPS1 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome
Urea cycle TAS Reactome





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