Gene description for AP1S2
Gene name adaptor-related protein complex 1, sigma 2 subunit
Gene symbol AP1S2
Other names/aliases DC22
MRX59
MRXS21
MRXS5
MRXSF
PGS
SIGMA1B
Species Homo sapiens
 Database cross references - AP1S2
ExoCarta ExoCarta_8905
Vesiclepedia VP_8905
Entrez Gene 8905
HGNC 560
MIM 300629
UniProt P56377  
 AP1S2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for AP1S2
Molecular Function
    protein binding GO:0005515 IPI
    clathrin adaptor activity GO:0035615 IEA
Biological Process
    intracellular protein transport GO:0006886 IEA
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    platelet dense granule organization GO:0060155 NAS
    melanosome assembly GO:1903232 NAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    lysosomal membrane GO:0005765 NAS
    lysosomal membrane GO:0005765 TAS
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    clathrin-coated pit GO:0005905 IEA
    AP-type membrane coat adaptor complex GO:0030119 TAS
    AP-1 adaptor complex GO:0030121 NAS
    cytoplasmic vesicle membrane GO:0030659 TAS
    trans-Golgi network membrane GO:0032588 NAS
    trans-Golgi network membrane GO:0032588 TAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified AP1S2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AP1S2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SYNRG 11276
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 AP2B1 163
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 HEATR5B 54497
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NOP58 51602
Affinity Capture-MS Homo sapiens
5 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HADHA 3030
Co-fractionation Homo sapiens
7 CPS1 1373
Affinity Capture-MS Homo sapiens
8 ABCG1  
Affinity Capture-MS Homo sapiens
9 XPO1 7514
Affinity Capture-MS Homo sapiens
10 STX8 9482
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SEC13 6396
Affinity Capture-MS Homo sapiens
12 CAV3 859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 UBE2M 9040
Affinity Capture-MS Homo sapiens
14 ATG9A 79065
Proximity Label-MS Homo sapiens
15 DLD 1738
Affinity Capture-MS Homo sapiens
16 AP1M1 8907
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DCAF13 25879
Affinity Capture-MS Homo sapiens
18 EGFR 1956
Affinity Capture-MS Homo sapiens
19 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
20 MAB21L2  
Two-hybrid Homo sapiens
21 FTH1 2495
Co-fractionation Homo sapiens
22 PI4K2A 55361
Affinity Capture-MS Homo sapiens
23 AFTPH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GGCX 2677
Affinity Capture-MS Homo sapiens
25 AP1M2 10053
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 AP1G2 8906
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NBEAL1 65065
Affinity Capture-MS Homo sapiens
29 AAGAB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PRDX3 10935
Co-fractionation Homo sapiens
31 SPATA33  
Affinity Capture-MS Homo sapiens
32 SLC43A3 29015
Affinity Capture-MS Homo sapiens
33 PPP6R2 9701
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 C19orf52  
Affinity Capture-MS Homo sapiens
35 CLTA 1211
Affinity Capture-MS Homo sapiens
36 AP1B1 162
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CCM2 83605
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NUDT3 11165
Affinity Capture-MS Homo sapiens
39 AP1G1 164
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
40 RNF13 11342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CLINT1 9685
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 POTEG  
Affinity Capture-MS Homo sapiens
43 CORT  
Affinity Capture-MS Homo sapiens
44 AP1AR 55435
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 COMT 1312
Affinity Capture-MS Homo sapiens
46 POTEB3 102724631
Affinity Capture-MS Homo sapiens
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