Gene description for KCTD1
Gene name potassium channel tetramerization domain containing 1
Gene symbol KCTD1
Other names/aliases C18orf5
SENS
Species Homo sapiens
 Database cross references - KCTD1
ExoCarta ExoCarta_284252
Vesiclepedia VP_284252
Entrez Gene 284252
HGNC 18249
MIM 613420
UniProt Q719H9  
 KCTD1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for KCTD1
Molecular Function
    transcription corepressor activity GO:0003714 IBA
    transcription corepressor activity GO:0003714 IDA
    protein binding GO:0005515 IPI
    transcription factor binding GO:0008134 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    negative regulation of DNA-templated transcription GO:0045892 IBA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    protein homooligomerization GO:0051260 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified KCTD1 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for KCTD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TFAP2B  
Affinity Capture-Western Homo sapiens
2 TRAPPC2 6399
Two-hybrid Homo sapiens
3 SDCBP 6386
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 EPM2AIP1 9852
Two-hybrid Homo sapiens
5 WNT3A 89780
Phenotypic Suppression Homo sapiens
6 TFAP2A  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
7 POLR1C 9533
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 CTNNB1 1499
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 PRKAA2 5563
Two-hybrid Homo sapiens
10 APC  
Phenotypic Suppression Homo sapiens
11 MORF4L2  
Two-hybrid Homo sapiens
12 TRAPPC2B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 SOX2  
Affinity Capture-MS Homo sapiens
14 PSMA1 5682
Two-hybrid Homo sapiens
15 TGM7  
Two-hybrid Homo sapiens
16 ACACA 31
Positive Genetic Homo sapiens
17 UBE2I 7329
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 LMO3  
Two-hybrid Homo sapiens
19 UBASH3A 53347
Two-hybrid Homo sapiens
20 WDYHV1  
Two-hybrid Homo sapiens
21 S100P 6286
Affinity Capture-MS Homo sapiens
22 TFAP2C 7022
Affinity Capture-Western Homo sapiens
23 ZBTB25  
Two-hybrid Homo sapiens
24 KCTD1 284252
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 LMO1  
Two-hybrid Homo sapiens
26 HECTD1 25831
Affinity Capture-MS Homo sapiens
27 KCTD15 79047
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 KCTD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 EXOSC1 51013
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 LNX1  
Two-hybrid Homo sapiens
View the network image/svg+xml



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