Gene description for EPHX4
Gene name epoxide hydrolase 4
Gene symbol EPHX4
Other names/aliases ABHD7
EH4
EPHXRP
Species Homo sapiens
 Database cross references - EPHX4
ExoCarta ExoCarta_253152
Vesiclepedia VP_253152
Entrez Gene 253152
HGNC 23758
UniProt Q8IUS5  
 EPHX4 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for EPHX4
Molecular Function
    epoxide hydrolase activity GO:0004301 IEA
    protein binding GO:0005515 IPI
    hydrolase activity GO:0016787 IBA
Subcellular Localization
    membrane GO:0016020 IEA
 Experiment description of studies that identified EPHX4 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for EPHX4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM14B  
Two-hybrid Homo sapiens
2 RNASE7 84659
Affinity Capture-MS Homo sapiens
3 SERPINA12  
Affinity Capture-MS Homo sapiens
4 TTYH1  
Affinity Capture-MS Homo sapiens
5 CD3D 915
Affinity Capture-MS Homo sapiens
6 MFSD4  
Affinity Capture-MS Homo sapiens
7 HS2ST1 9653
Affinity Capture-MS Homo sapiens
8 TSPAN15 23555
Affinity Capture-MS Homo sapiens
9 RBM6 10180
Affinity Capture-MS Homo sapiens
10 KLK7 5650
Affinity Capture-MS Homo sapiens
11 CDSN 1041
Affinity Capture-MS Homo sapiens
12 SBSN 374897
Affinity Capture-MS Homo sapiens
13 ARMC6 93436
Affinity Capture-MS Homo sapiens
14 PBXIP1 57326
Affinity Capture-MS Homo sapiens
15 GPR45  
Affinity Capture-MS Homo sapiens
16 GPR182  
Affinity Capture-MS Homo sapiens
17 PCDHA12  
Affinity Capture-MS Homo sapiens
18 CGRRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SERPINB7 8710
Affinity Capture-MS Homo sapiens
20 PCDHB3  
Affinity Capture-MS Homo sapiens
21 TMEM59 9528
Affinity Capture-MS Homo sapiens
22 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EPHX4 is involved
No pathways found





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