Experiment description of studies that identified SERPINB7 in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for SERPINB7

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PRSS16 10279 Affinity Capture-MS Homo sapiens 2 DEFB106A   Affinity Capture-MS Homo sapiens 3 NONO 4841 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 IFNA4   Affinity Capture-MS Homo sapiens 5 RHBDD1 84236 Affinity Capture-MS Homo sapiens 6 KLHL11   Affinity Capture-MS Homo sapiens 7 ADSS 159 Affinity Capture-MS Homo sapiens 8 SEP15 9403 Affinity Capture-MS Homo sapiens 9 VWA3B 200403 Affinity Capture-MS Homo sapiens 10 AMTN   Affinity Capture-MS Homo sapiens 11 SCGB1D1   Affinity Capture-MS Homo sapiens 12 CCNC   Affinity Capture-MS Homo sapiens 13 ZDHHC19   Affinity Capture-MS Homo sapiens 14 CCR1   Affinity Capture-MS Homo sapiens 15 AGPAT1 10554 Affinity Capture-MS Homo sapiens 16 POLR2L 5441 Affinity Capture-MS Homo sapiens 17 DDX19B 11269 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 TBC1D22B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 MCL1 4170 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 SLC37A4 2542 Affinity Capture-MS Homo sapiens 21 EPHX4 253152 Affinity Capture-MS Homo sapiens 22 PHF8   Affinity Capture-MS Homo sapiens 23 ATP6AP2 10159 Affinity Capture-MS Homo sapiens 24 CDK15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 C1orf35   Affinity Capture-MS Homo sapiens 26 SSBP2   Affinity Capture-MS Homo sapiens 27 ZC3HC1   Affinity Capture-MS Homo sapiens 28 LRRC10   Affinity Capture-MS Homo sapiens 29 PLK2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 FTH1 2495 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 MKI67   Affinity Capture-MS Homo sapiens 32 SOX2   Affinity Capture-MS Homo sapiens 33 PI4KA 5297 Affinity Capture-MS Homo sapiens 34 C21orf59 56683 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 HEY2   Affinity Capture-MS Homo sapiens 36 RNF115   Affinity Capture-MS Homo sapiens 37 CHST10   Affinity Capture-MS Homo sapiens 38 RPS19BP1   Affinity Capture-MS Homo sapiens 39 MBNL1 4154 Affinity Capture-MS Homo sapiens 40 KIR3DS1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 PI4KAP1   Affinity Capture-MS Homo sapiens 42 GDF5 8200 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 THAP4 51078 Affinity Capture-MS Homo sapiens 44 TEFM   Affinity Capture-MS Homo sapiens 45 SFR1 119392 Affinity Capture-MS Homo sapiens 46 PIGT 51604 Affinity Capture-MS Homo sapiens 47 PINK1   Affinity Capture-MS Homo sapiens 48 GOT1 2805 Affinity Capture-MS Homo sapiens 49 SARAF   Affinity Capture-MS Homo sapiens 50 SSUH2   Affinity Capture-MS Homo sapiens 51 NUDT3 11165 Affinity Capture-MS Homo sapiens 52 OR2A4 79541 Affinity Capture-MS Homo sapiens 53 MOGAT3   Affinity Capture-MS Homo sapiens 54 FANCG 2189 Affinity Capture-MS Homo sapiens 55 GABPA 2551 Affinity Capture-MS Homo sapiens 56 FOXJ3   Affinity Capture-MS Homo sapiens 57 C18orf21   Affinity Capture-MS Homo sapiens 58 GUCY1A3   Affinity Capture-MS Homo sapiens 59 STX17 55014 Affinity Capture-MS Homo sapiens 60 RIPPLY3   Affinity Capture-MS Homo sapiens 61 GALNT9   Affinity Capture-MS Homo sapiens 62 PHF11 51131 Affinity Capture-MS Homo sapiens View the network

Pathways in which SERPINB7 is involved

No pathways found