Gene description for ABCA3
Gene name ATP-binding cassette, sub-family A (ABC1), member 3
Gene symbol ABCA3
Other names/aliases ABC-C
ABC3
EST111653
LBM180
SMDP3
Species Homo sapiens
 Database cross references - ABCA3
ExoCarta ExoCarta_21
Vesiclepedia VP_21
Entrez Gene 21
HGNC 33
MIM 601615
UniProt Q99758  
 ABCA3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ABCA3
Molecular Function
    lipid transporter activity GO:0005319 IBA
    lipid transporter activity GO:0005319 TAS
    ATP binding GO:0005524 IEA
    ABC-type xenobiotic transporter activity GO:0008559 IMP
    ATP hydrolysis activity GO:0016887 IDA
    ATPase-coupled transmembrane transporter activity GO:0042626 IBA
    phosphatidylcholine transfer activity GO:0120019 IMP
    phosphatidylcholine flippase activity GO:0140345 IMP
Biological Process
    xenobiotic transmembrane transport GO:0006855 IMP
    lipid transport GO:0006869 IBA
    lipid transport GO:0006869 TAS
    response to xenobiotic stimulus GO:0009410 TAS
    positive regulation of cholesterol efflux GO:0010875 IMP
    phospholipid transport GO:0015914 IMP
    lung development GO:0030324 ISS
    positive regulation of protein homooligomerization GO:0032464 IDA
    lipid translocation GO:0034204 IEA
    xenobiotic transport GO:0042908 IMP
    surfactant homeostasis GO:0043129 ISS
    phosphatidylcholine metabolic process GO:0046470 IMP
    phosphatidylcholine metabolic process GO:0046470 ISS
    phosphatidylglycerol metabolic process GO:0046471 ISS
    xenobiotic export from cell GO:0046618 IMP
    regulation of lipid biosynthetic process GO:0046890 IEA
    response to glucocorticoid GO:0051384 IEA
    phospholipid homeostasis GO:0055091 IEA
    organelle assembly GO:0070925 ISS
    intermembrane lipid transfer GO:0120009 IEA
    regulation of phosphatidylcholine metabolic process GO:0150172 IMP
    positive regulation of phospholipid efflux GO:1902995 IMP
    positive regulation of phospholipid transport GO:2001140 IMP
Subcellular Localization
    extracellular space GO:0005615 HDA
    lysosomal membrane GO:0005765 IEA
    late endosome GO:0005770 IDA
    plasma membrane GO:0005886 IDA
    cytoplasmic vesicle membrane GO:0030659 IDA
    multivesicular body membrane GO:0032585 IEA
    lamellar body GO:0042599 IDA
    intracellular membrane-bounded organelle GO:0043231 IBA
    alveolar lamellar body GO:0097208 IDA
    lamellar body membrane GO:0097232 IDA
    lamellar body membrane GO:0097232 TAS
    alveolar lamellar body membrane GO:0097233 IDA
 Experiment description of studies that identified ABCA3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ABCA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 NRG1 3084
Affinity Capture-MS Homo sapiens
3 NKAIN1  
Affinity Capture-MS Homo sapiens
4 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 KLRC2  
Affinity Capture-MS Homo sapiens
6 PTH1R  
Affinity Capture-MS Homo sapiens
7 TMEM59 9528
Affinity Capture-MS Homo sapiens
8 TTYH1  
Affinity Capture-MS Homo sapiens
9 ARRDC4  
Affinity Capture-MS Homo sapiens
10 HLA-E 3133
Affinity Capture-MS Homo sapiens
11 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ZDHHC12  
Affinity Capture-MS Homo sapiens
13 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ASIC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 MANSC1 54682
Affinity Capture-MS Homo sapiens
16 TMPRSS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TMEM9B 56674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GPR114  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ATP1B3 483
Affinity Capture-MS Homo sapiens
20 GNAI1 2770
Affinity Capture-MS Homo sapiens
21 EVA1C  
Affinity Capture-MS Homo sapiens
22 CHRNB2  
Affinity Capture-MS Homo sapiens
23 B3GAT1  
Proximity Label-MS Homo sapiens
24 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TMEM130  
Affinity Capture-MS Homo sapiens
26 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 HTR2B  
Two-hybrid Homo sapiens
28 FAM189A2  
Affinity Capture-MS Homo sapiens
29 PCDHB16  
Affinity Capture-MS Homo sapiens
30 MARCH4  
Affinity Capture-MS Homo sapiens
31 RPN1 6184
Proximity Label-MS Homo sapiens
32 MPPE1 65258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 IL17RC 84818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 UPK1A 11045
Affinity Capture-MS Homo sapiens
35 SPINT2 10653
Affinity Capture-MS Homo sapiens
36 PMEL 6490
Affinity Capture-MS Homo sapiens
37 C3orf52  
Affinity Capture-MS Homo sapiens
38 ZACN  
Affinity Capture-MS Homo sapiens
39 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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