ExoCarta: Gene summary

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Gene description for ABCA3
Gene name	ATP-binding cassette, sub-family A (ABC1), member 3
Gene symbol	ABCA3
Other names/aliases	ABC-C ABC3 EST111653 LBM180 SMDP3
Species	Homo sapiens
Database cross references - ABCA3
ExoCarta	ExoCarta_21
Entrez Gene	21
HGNC	33
MIM	601615
UniProt	Q99758
ABCA3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells	26054723
Hepatocellular carcinoma cells	26054723

Gene ontology annotations for ABCA3

Molecular Function
	transporter activity	GO:0005215	TAS
	ATP binding	GO:0005524	IEA
	ATPase activity, coupled to transmembrane movement of substances	GO:0042626	TAS
Biological Process
	transmembrane transport	GO:0055085	TAS
	transport	GO:0006810	IDA
	lipid transport	GO:0006869	IBA
	metabolic process	GO:0008152	IEA
	response to drug	GO:0042493	TAS
Subcellular Localization
	integral component of membrane	GO:0016021	IEA
	alveolar lamellar body membrane	GO:0097233	IEA
	alveolar lamellar body	GO:0097208	IDA
	plasma membrane	GO:0005886	TAS
	membrane	GO:0016020	TAS
	extracellular space	GO:0005615	IDA

Experiment description of studies that identified ABCA3 in exosomes

Experiment ID

234

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|Alix\|HSC70\|GAPDH	EV Cytosolic markers
✘	EV Membrane markers
✔ HSP90B1	EV Negative markers
✔ qNano	EV Particle analysis

Identified molecule

mRNA

Identification method

RNA Sequencing

PubMed ID

26054723

Organism

Homo sapiens

Experiment description

Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs

Authors

He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N

Journal name

Carcinogenesis

Publication year

2015

Sample

Hepatocellular carcinoma cells

Sample name

HKCI-C3

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.19 g/mL

Molecules identified in the study

Protein
RNA

Methods used in the study

Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing