Gene description for COLEC12
Gene name collectin sub-family member 12
Gene symbol COLEC12
Other names/aliases CLP1
NSR2
SCARA4
SRCL
Species Homo sapiens
 Database cross references - COLEC12
ExoCarta ExoCarta_81035
Vesiclepedia VP_81035
Entrez Gene 81035
HGNC 16016
MIM 607621
UniProt Q5KU26  
 COLEC12 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for COLEC12
Molecular Function
    scavenger receptor activity GO:0005044 IDA
    scavenger receptor activity GO:0005044 TAS
    protein binding GO:0005515 IPI
    galactose binding GO:0005534 NAS
    low-density lipoprotein particle binding GO:0030169 IBA
    low-density lipoprotein particle binding GO:0030169 IDA
    pattern recognition receptor activity GO:0038187 IBA
    pattern recognition receptor activity GO:0038187 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    phagocytosis, recognition GO:0006910 IBA
    phagocytosis, recognition GO:0006910 IDA
    defense response GO:0006952 TAS
    carbohydrate mediated signaling GO:0009756 NAS
    toll-like receptor 3 signaling pathway GO:0034138 IMP
    defense response to bacterium GO:0042742 IDA
    plasma membrane raft organization GO:0044857 IMP
    innate immune response GO:0045087 TAS
    cellular response to exogenous dsRNA GO:0071360 IMP
    cellular response to exogenous dsRNA GO:0071360 IMP
Subcellular Localization
    collagen trimer GO:0005581 IEA
    extracellular space GO:0005615 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    endocytic vesicle membrane GO:0030666 TAS
    collagen-containing extracellular matrix GO:0062023 IBA
 Experiment description of studies that identified COLEC12 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for COLEC12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
2 MMP2 4313
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 HOMER1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CLEC4F  
Affinity Capture-MS Homo sapiens
5 LAMB1 3912
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 C1QTNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 C1QTNF1 114897
Affinity Capture-MS Homo sapiens
9 LAIR2  
Affinity Capture-MS Homo sapiens
10 LAMTOR1 55004
Proximity Label-MS Homo sapiens
11 CLGN 1047
Affinity Capture-MS Homo sapiens
12 TMEM59 9528
Affinity Capture-MS Homo sapiens
13 CANX 821
Affinity Capture-MS Homo sapiens
14 KIF14 9928
Affinity Capture-MS Homo sapiens
15 LAMP3  
Proximity Label-MS Homo sapiens
16 LGALS1 3956
Affinity Capture-MS Homo sapiens
17 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LGALS7 3963
Affinity Capture-MS Homo sapiens
19 FRMD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ARHGAP26 23092
Affinity Capture-MS Homo sapiens
21 CLEC2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 ZC4H2 55906
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TSPAN15 23555
Affinity Capture-MS Homo sapiens
25 LMAN2L 81562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 IKBIP 121457
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FAM189B  
Affinity Capture-MS Homo sapiens
28 TMEM126A 84233
Affinity Capture-MS Homo sapiens
29 LAMP2 3920
Proximity Label-MS Homo sapiens
30 FXYD6  
Affinity Capture-MS Homo sapiens
31 TMX1 81542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 S1PR1 1901
Affinity Capture-MS Homo sapiens
33 CLEC12A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 FRMD5 84978
Affinity Capture-MS Homo sapiens
35 SLC30A4 7782
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 APP 351
Reconstituted Complex Homo sapiens
38 SMIM5  
Affinity Capture-MS Homo sapiens
39 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RAB4A 5867
Proximity Label-MS Homo sapiens
41 ATG16L1 55054
Affinity Capture-MS Homo sapiens
42 RAB2A 5862
Proximity Label-MS Homo sapiens
43 CGRRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SCARA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 FBXO2 26232
Affinity Capture-MS Homo sapiens
46 KLRG2 346689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 EXOC1 55763
Two-hybrid Homo sapiens
48 DPH2 1802
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RAB11A 8766
Proximity Label-MS Homo sapiens
50 COL8A2 1296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 STX7 8417
Proximity Label-MS Homo sapiens
53 B3GAT3 26229
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PDZK1 5174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 MMP9 4318
Affinity Capture-MS Homo sapiens
57 RAB5C 5878
Proximity Label-MS Homo sapiens
58 PRC1 9055
Affinity Capture-MS Homo sapiens
59 CSPG5 10675
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TLR1 7096
Affinity Capture-MS Homo sapiens
61 DRD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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