ExoCarta: Gene summary

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Gene description for COL8A2
Gene name	collagen, type VIII, alpha 2
Gene symbol	COL8A2
Other names/aliases	FECD FECD1 PPCD PPCD2
Species	Homo sapiens
Database cross references - COL8A2
ExoCarta	ExoCarta_1296
Vesiclepedia	VP_1296
Entrez Gene	1296
HGNC	2216
MIM	120252
UniProt	P25067
COL8A2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Mesenchymal stem cells	36408942

Gene ontology annotations for COL8A2

Molecular Function
	extracellular matrix structural constituent	GO:0005201	NAS
	extracellular matrix structural constituent conferring tensile strength	GO:0030020	IBA
	extracellular matrix structural constituent conferring tensile strength	GO:0030020	RCA
	protein-macromolecule adaptor activity	GO:0030674	NAS
Biological Process
	angiogenesis	GO:0001525	IEA
	endothelial cell proliferation	GO:0001935	IEA
	extracellular matrix organization	GO:0030198	IBA
	extracellular matrix organization	GO:0030198	NAS
	camera-type eye morphogenesis	GO:0048593	IEA
	cell-cell adhesion	GO:0098609	NAS
Subcellular Localization
	extracellular region	GO:0005576	TAS
	collagen trimer	GO:0005581	IEA
	basement membrane	GO:0005604	NAS
	extracellular space	GO:0005615	IBA
	endoplasmic reticulum lumen	GO:0005788	TAS
	extracellular matrix	GO:0031012	NAS
	collagen-containing extracellular matrix	GO:0062023	HDA
	collagen-containing extracellular matrix	GO:0062023	HDA
	collagen-containing extracellular matrix	GO:0062023	IBA

Experiment description of studies that identified COL8A2 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for COL8A2

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

COL14A1

7373

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

P3H1

64175

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

COLGALT2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

COL4A5

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GPX8

493869

Two-hybrid

Homo sapiens

COLEC12

81035

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FNDC9

Two-hybrid

Homo sapiens

COL4A2

1284

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MVP

9961

Affinity Capture-MS

Homo sapiens

COL2A1

1280

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLHL8

Affinity Capture-MS

Homo sapiens

P4HA2

8974

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

COL4A6

1288

Affinity Capture-MS

Homo sapiens

PEX12

Two-hybrid

Homo sapiens

COL18A1

80781

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CREB3L1

Two-hybrid

Homo sapiens

COL6A1

1291

Affinity Capture-MS

Homo sapiens

FN1

2335

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FKBP14

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

COL6A2

1292

Affinity Capture-MS

Homo sapiens

GPR152

Two-hybrid

Homo sapiens

FAM209A

200232

Two-hybrid

Homo sapiens

ATP6V0E1

Two-hybrid

Homo sapiens

PLOD2

5352

Affinity Capture-MS

Homo sapiens

BSND

Two-hybrid

Homo sapiens

TFAM

7019

Two-hybrid

Homo sapiens

COL5A1

1289

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HSPA5

3309

Affinity Capture-MS

Homo sapiens

CRTAP

10491

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MUC1

4582

Two-hybrid

Homo sapiens

COL8A1

1295

Reconstituted Complex

Homo sapiens

PARP4

143

Affinity Capture-MS

Homo sapiens

SGTA

6449

Two-hybrid

Homo sapiens

ARL13B

200894

Two-hybrid

Homo sapiens

COL12A1

1303

Affinity Capture-MS

Homo sapiens

TMX2

51075

Two-hybrid

Homo sapiens

DARS2

55157

Two-hybrid

Homo sapiens

View the network

Pathways in which COL8A2 is involved

Pathway	Evidence	Source
Assembly of collagen fibrils and other multimeric structures	TAS	Reactome
Collagen biosynthesis and modifying enzymes	TAS	Reactome
Collagen chain trimerization	TAS	Reactome
Collagen degradation	TAS	Reactome
Collagen degradation	IEA	Reactome
Collagen formation	TAS	Reactome
Degradation of the extracellular matrix	TAS	Reactome
Degradation of the extracellular matrix	IEA	Reactome
Extracellular matrix organization	TAS	Reactome
Extracellular matrix organization	IEA	Reactome
Integrin cell surface interactions	TAS	Reactome

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