Gene description for CHSY1
Gene name chondroitin sulfate synthase 1
Gene symbol CHSY1
Other names/aliases CHSY
CSS1
ChSy-1
TPBS
Species Homo sapiens
 Database cross references - CHSY1
ExoCarta ExoCarta_22856
Vesiclepedia VP_22856
Entrez Gene 22856
HGNC 17198
MIM 608183
UniProt Q86X52  
 CHSY1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CHSY1
Molecular Function
    metal ion binding GO:0046872 IEA
    glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity GO:0047238 IBA
    glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity GO:0047238 IDA
    glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity GO:0047238 TAS
    N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity GO:0050510 IDA
    N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity GO:0050510 TAS
Biological Process
    chondrocyte development GO:0002063 IEA
    proximal/distal pattern formation GO:0009954 IEA
    chondroitin sulfate biosynthetic process GO:0030206 IBA
    chondroitin sulfate biosynthetic process GO:0030206 IDA
    chondroitin sulfate biosynthetic process GO:0030206 TAS
    negative regulation of ossification GO:0030279 IMP
    response to nutrient levels GO:0031667 IEA
    positive regulation of smoothened signaling pathway GO:0045880 IEA
    chondroitin sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process GO:0050653 IDA
    sulfation GO:0051923 IEA
    bone morphogenesis GO:0060349 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 IDA
    membrane GO:0016020 HDA
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified CHSY1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CHSY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 C7orf34  
Affinity Capture-MS Homo sapiens
3 DKK3 27122
Affinity Capture-MS Homo sapiens
4 HSPA5 3309
Affinity Capture-MS Homo sapiens
5 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TMEM106A  
Affinity Capture-MS Homo sapiens
7 CCL3  
Affinity Capture-MS Homo sapiens
8 KCNA4  
Affinity Capture-MS Homo sapiens
9 LCN6  
Affinity Capture-MS Homo sapiens
10 FOXP1 27086
Protein-RNA Homo sapiens
11 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 C1orf54  
Affinity Capture-MS Homo sapiens
13 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TMEM59 9528
Affinity Capture-MS Homo sapiens
15 PON2 5445
Affinity Capture-MS Homo sapiens
16 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PDGFRA 5156
Affinity Capture-MS Homo sapiens
18 GOLT1B 51026
Affinity Capture-MS Homo sapiens
19 NRSN1  
Affinity Capture-MS Homo sapiens
20 NIPAL1  
Affinity Capture-MS Homo sapiens
21 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
22 MIA2  
Affinity Capture-MS Homo sapiens
23 FAXC  
Affinity Capture-MS Homo sapiens
24 MYCBPAP 84073
Affinity Capture-MS Homo sapiens
25 EIF2B5 8893
Affinity Capture-MS Homo sapiens
26 DCN 1634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CLEC12B  
Affinity Capture-MS Homo sapiens
28 SAAL1 113174
Affinity Capture-MS Homo sapiens
29 TMEM154  
Affinity Capture-MS Homo sapiens
30 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TMEM79  
Affinity Capture-MS Homo sapiens
32 KCNA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SLC39A5 283375
Affinity Capture-MS Homo sapiens
36 FOXN3 1112
Affinity Capture-MS Homo sapiens
37 OLFML2B 25903
Affinity Capture-MS Homo sapiens
38 SLC1A1 6505
Affinity Capture-MS Homo sapiens
39 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SDF4 51150
Affinity Capture-MS Homo sapiens
41 CHSY3 337876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ATP2A3 489
Affinity Capture-MS Homo sapiens
43 ZMYM2  
Affinity Capture-MS Homo sapiens
44 SLC39A8 64116
Affinity Capture-MS Homo sapiens
45 C1QTNF7  
Affinity Capture-MS Homo sapiens
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