Gene description for NOP14
Gene name NOP14 nucleolar protein
Gene symbol NOP14
Other names/aliases C4orf9
NOL14
RES4-25
RES425
UTP2
Species Homo sapiens
 Database cross references - NOP14
ExoCarta ExoCarta_8602
Entrez Gene 8602
HGNC 16821
MIM 611526
UniProt P78316  
 NOP14 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for NOP14
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    snoRNA binding GO:0030515 ISS
    enzyme binding GO:0019899 IPI
Biological Process
    maturation of SSU-rRNA GO:0030490 IBA
    maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000462 ISS
    rRNA processing GO:0006364 ISS
    endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000447 ISS
    endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000480 ISS
    endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000472 ISS
    ribosomal small subunit biogenesis GO:0042274 ISS
Subcellular Localization
    90S preribosome GO:0030686 ISS
    Noc4p-Nop14p complex GO:0030692 ISS
    membrane GO:0016020 IDA
    nucleus GO:0005634 IDA
    small-subunit processome GO:0032040 ISS
    nucleolus GO:0005730 ISS
    mitochondrion GO:0005739 ISS
 Experiment description of studies that identified NOP14 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for NOP14
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NOP14 is involved
No pathways found





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