Gene description for CPD
Gene name carboxypeptidase D
Gene symbol CPD
Other names/aliases GP180
Species Homo sapiens
 Database cross references - CPD
ExoCarta ExoCarta_1362
Vesiclepedia VP_1362
Entrez Gene 1362
HGNC 2301
MIM 603102
UniProt O75976  
 CPD identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Saliva 19199708    
 Gene ontology annotations for CPD
Molecular Function
    metallocarboxypeptidase activity GO:0004181 IBA
    serine-type carboxypeptidase activity GO:0004185 TAS
    zinc ion binding GO:0008270 IEA
Biological Process
    peptide metabolic process GO:0006518 IBA
    protein processing GO:0016485 IBA
Subcellular Localization
    extracellular space GO:0005615 IBA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 HDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CPD in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
10
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for CPD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 AP2B1 163
Proximity Label-MS Homo sapiens
4 LGR4 55366
Affinity Capture-MS Homo sapiens
5 AATF  
Two-hybrid Homo sapiens
6 METTL7A 25840
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 ATG9A 79065
Proximity Label-MS Homo sapiens
9 GJA1 2697
Proximity Label-MS Homo sapiens
10 GAK 2580
Proximity Label-MS Homo sapiens
11 MTG2  
Proximity Label-MS Homo sapiens
12 C9orf78 51759
Affinity Capture-MS Homo sapiens
13 HSD17B11 51170
Proximity Label-MS Homo sapiens
14 LAMP2 3920
Proximity Label-MS Homo sapiens
15 DNAJC25 548645
Proximity Label-MS Homo sapiens
16 STX4 6810
Proximity Label-MS Homo sapiens
17 APEX1 328
Affinity Capture-RNA Homo sapiens
18 ASB17  
Affinity Capture-MS Homo sapiens
19 B3GAT1  
Proximity Label-MS Homo sapiens
20 KIF23 9493
Affinity Capture-MS Homo sapiens
21 LGALS1 3956
Affinity Capture-MS Homo sapiens
22 RAB3B 5865
Proximity Label-MS Homo sapiens
23 STX7 8417
Proximity Label-MS Homo sapiens
24 UBL4A 8266
Affinity Capture-MS Homo sapiens
25 OCLN 100506658
Proximity Label-MS Homo sapiens
26 TACSTD2 4070
Affinity Capture-MS Homo sapiens
27 UFSP2 55325
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CANX 821
Affinity Capture-MS Homo sapiens
29 PRTN3 5657
Affinity Capture-MS Homo sapiens
30 RAB35 11021
Proximity Label-MS Homo sapiens
31 TMEM132D  
Two-hybrid Homo sapiens
32 EBAG9 9166
Proximity Label-MS Homo sapiens
33 MARCKS 4082
Proximity Label-MS Homo sapiens
34 LAMTOR1 55004
Proximity Label-MS Homo sapiens
35 TNF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LMAN1 3998
Proximity Label-MS Homo sapiens
37 RAB5A 5868
Proximity Label-MS Homo sapiens
38 CXADR 1525
Proximity Label-MS Homo sapiens
39 IL1R2  
Affinity Capture-MS Homo sapiens
40 RAB4A 5867
Proximity Label-MS Homo sapiens
41 DNAJC5 80331
Proximity Label-MS Homo sapiens
42 DIRAS3  
Proximity Label-MS Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 FOXK2  
Affinity Capture-MS Homo sapiens
45 RAD18  
Co-localization Homo sapiens
46 STX6 10228
Proximity Label-MS Homo sapiens
47 RPN1 6184
Proximity Label-MS Homo sapiens
48 ATP4A 495
Affinity Capture-MS Homo sapiens
49 ELOVL5 60481
Proximity Label-MS Homo sapiens
50 SEC61B 10952
Proximity Label-MS Homo sapiens
51 AP1M1 8907
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 NHLRC3 387921
Affinity Capture-MS Homo sapiens
53 RPN2 6185
Proximity Label-MS Homo sapiens
54 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 LRRC59 55379
Proximity Label-MS Homo sapiens
56 ARRDC4  
Affinity Capture-MS Homo sapiens
57 LAMP1 3916
Proximity Label-MS Homo sapiens
58 ATG12  
Proximity Label-MS Homo sapiens
59 GAST  
Affinity Capture-MS Homo sapiens
60 HSD3B7 80270
Proximity Label-MS Homo sapiens
61 STIM1 6786
Proximity Label-MS Homo sapiens
62 HOOK3 84376
Proximity Label-MS Homo sapiens
63 ARF6 382
Proximity Label-MS Homo sapiens
64 FBXO6 26270
Affinity Capture-MS Homo sapiens
65 ATP2A1 487
Proximity Label-MS Homo sapiens
66 FBXO2 26232
Affinity Capture-MS Homo sapiens
67 RAB9A 9367
Proximity Label-MS Homo sapiens
68 GOLGA1  
Proximity Label-MS Homo sapiens
69 RHOB 388
Proximity Label-MS Homo sapiens
70 KIAA0368 23392
Two-hybrid Homo sapiens
71 IFNA21  
Affinity Capture-MS Homo sapiens
72 CRLF2 64109
Affinity Capture-MS Homo sapiens
73 RNF43  
Proximity Label-MS Homo sapiens
74 ANKFY1 51479
Proximity Label-MS Homo sapiens
75 PPM1H  
Proximity Label-MS Homo sapiens
76 CD1B 910
Affinity Capture-MS Homo sapiens
77 RYK 6259
Affinity Capture-MS Homo sapiens
78 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
79 COX8A  
Proximity Label-MS Homo sapiens
80 ERGIC2 51290
Proximity Label-MS Homo sapiens
81 RAB2A 5862
Proximity Label-MS Homo sapiens
82 SEC62 7095
Proximity Label-MS Homo sapiens
83 RAB7A 7879
Proximity Label-MS Homo sapiens
84 RAB11A 8766
Proximity Label-MS Homo sapiens
85 TCF3  
Affinity Capture-MS Homo sapiens
86 EPHA2 1969
Proximity Label-MS Homo sapiens
87 DNAJC1 64215
Proximity Label-MS Homo sapiens
88 KRAS 3845
Proximity Label-MS Homo sapiens
89 RAB5C 5878
Proximity Label-MS Homo sapiens
90 CAV1 857
Proximity Label-MS Homo sapiens
91 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here