Gene description for HSD3B2
Gene name hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Gene symbol HSD3B2
Other names/aliases HSD3B
HSDB
SDR11E2
Species Homo sapiens
 Database cross references - HSD3B2
ExoCarta ExoCarta_3284
Vesiclepedia VP_3284
Entrez Gene 3284
HGNC 5218
MIM 613890
UniProt P26439  
 HSD3B2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for HSD3B2
Molecular Function
    3-beta-hydroxy-delta5-steroid dehydrogenase (NAD+) activity GO:0003854 IDA
    3-beta-hydroxy-delta5-steroid dehydrogenase (NAD+) activity GO:0003854 IMP
    steroid delta-isomerase activity GO:0004769 IDA
    steroid delta-isomerase activity GO:0004769 IMP
    protein binding GO:0005515 IPI
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IBA
Biological Process
    steroid biosynthetic process GO:0006694 IBA
    steroid biosynthetic process GO:0006694 IDA
    steroid biosynthetic process GO:0006694 IMP
    androgen biosynthetic process GO:0006702 IMP
    C21-steroid hormone metabolic process GO:0008207 IBA
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial intermembrane space GO:0005758 ISS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 IMP
    endoplasmic reticulum GO:0005783 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 NAS
    smooth endoplasmic reticulum membrane GO:0030868 ISS
    mitochondrial membrane GO:0031966 NAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    intercellular bridge GO:0045171 IDA
 Experiment description of studies that identified HSD3B2 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for HSD3B2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WBP11  
Affinity Capture-MS Homo sapiens
2 HIST2H3PS2 440686
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 DDX46 9879
Affinity Capture-MS Homo sapiens
4 TCEA1 6917
Affinity Capture-MS Homo sapiens
5 LETM1 3954
Affinity Capture-MS Homo sapiens
6 CTSF 8722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ATP4A 495
Affinity Capture-MS Homo sapiens
8 TOP2A 7153
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 WDR36 134430
Affinity Capture-MS Homo sapiens
10 NARS 4677
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TMEM38A 79041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MTHFR 4524
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PC 5091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 HSD3B1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TBL3 10607
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 VRK1 7443
Affinity Capture-MS Homo sapiens
18 TOP2B 7155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 H2AFY 9555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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