Gene description for CA9
Gene name carbonic anhydrase IX
Gene symbol CA9
Other names/aliases CAIX
MN
Species Homo sapiens
 Database cross references - CA9
ExoCarta ExoCarta_768
Vesiclepedia VP_768
Entrez Gene 768
HGNC 1383
MIM 603179
UniProt Q16790  
 CA9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
 Gene ontology annotations for CA9
Molecular Function
    carbonate dehydratase activity GO:0004089 IBA
    carbonate dehydratase activity GO:0004089 IDA
    carbonate dehydratase activity GO:0004089 IMP
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    molecular function activator activity GO:0140677 IDA
Biological Process
    response to hypoxia GO:0001666 IEA
    morphogenesis of an epithelium GO:0002009 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to testosterone GO:0033574 IEA
    secretion GO:0046903 IEA
Subcellular Localization
    nucleolus GO:0005730 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    basolateral plasma membrane GO:0016323 IEA
    microvillus membrane GO:0031528 IEA
 Experiment description of studies that identified CA9 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 282
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 285
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for CA9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RTCB 51493
Affinity Capture-MS Homo sapiens
2 SRPRB 58477
Affinity Capture-MS Homo sapiens
3 IPO5 3843
Affinity Capture-MS Homo sapiens
4 HADHA 3030
Affinity Capture-MS Homo sapiens
5 XPO1 7514
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 IPO7 10527
Affinity Capture-MS Homo sapiens
7 TNPO1 3842
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 CTNNB1 1499
Affinity Capture-Western Homo sapiens
9 CAND2 23066
Affinity Capture-MS Homo sapiens
10 ATP5A1 498
Affinity Capture-MS Homo sapiens
11 CSE1L 1434
Affinity Capture-MS Homo sapiens
12 KPNA2 3838
Affinity Capture-MS Homo sapiens
13 CAND1 55832
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 ATXN10 25814
Affinity Capture-MS Homo sapiens
15 G3BP2 9908
Affinity Capture-MS Homo sapiens
16 SAAL1 113174
Affinity Capture-MS Homo sapiens
17 RPS5 6193
Affinity Capture-MS Homo sapiens
18 ACACA 31
Affinity Capture-MS Homo sapiens
19 XPO5 57510
Affinity Capture-MS Homo sapiens
20 HEATR3 55027
Affinity Capture-MS Homo sapiens
21 CDH1 999
Affinity Capture-Western Homo sapiens
22 IPO4 79711
Affinity Capture-MS Homo sapiens
23 FLOT1 10211
Co-fractionation Homo sapiens
24 TNPO3 23534
Affinity Capture-MS Homo sapiens
25 CSRP1 1465
Two-hybrid Homo sapiens
26 XPOT 11260
Affinity Capture-MS Homo sapiens
27 GLRX3 10539
Two-hybrid Homo sapiens
28 STAT3 6774
Co-localization Homo sapiens
29 ATP4A 495
Affinity Capture-MS Homo sapiens
30 CTNNA1 1495
Affinity Capture-Western Homo sapiens
31 CA2 760
Affinity Capture-MS Homo sapiens
32 ATP5B 506
Affinity Capture-MS Homo sapiens
33 IPO9 55705
Affinity Capture-MS Homo sapiens
34 HIF1A 3091
Co-localization Homo sapiens
View the network image/svg+xml



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