Gene description for CAND2
Gene name cullin-associated and neddylation-dissociated 2 (putative)
Gene symbol CAND2
Other names/aliases TIP120B
Tp120b
Species Homo sapiens
 Database cross references - CAND2
ExoCarta ExoCarta_23066
Entrez Gene 23066
HGNC 30689
MIM 610403
UniProt O75155  
 CAND2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for CAND2
Molecular Function
    TBP-class protein binding GO:0017025 IEA
    protein binding GO:0005515 IPI
Biological Process
    protein ubiquitination GO:0016567 IBA
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    transcription, DNA-templated GO:0006351 IEA
    SCF complex assembly GO:0010265 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    intracellular GO:0005622 IDA
 Experiment description of studies that identified CAND2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for CAND2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT3  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 UBE3C 9690
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which CAND2 is involved
No pathways found





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