Gene description for WFS1
Gene name Wolfram syndrome 1 (wolframin)
Gene symbol WFS1
Other names/aliases CTRCT41
WFRS
WFS
WFSL
Species Homo sapiens
 Database cross references - WFS1
ExoCarta ExoCarta_7466
Vesiclepedia VP_7466
Entrez Gene 7466
HGNC 12762
MIM 606201
UniProt O76024  
 WFS1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for WFS1
Molecular Function
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ubiquitin protein ligase binding GO:0031625 IDA
    calcium-dependent protein binding GO:0048306 IEA
    ATPase binding GO:0051117 IPI
    proteasome binding GO:0070628 IEA
    DNA-binding transcription factor binding GO:0140297 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    kidney development GO:0001822 IMP
    renal water homeostasis GO:0003091 IMP
    ER overload response GO:0006983 IC
    ER overload response GO:0006983 TAS
    visual perception GO:0007601 IMP
    sensory perception of sound GO:0007605 IMP
    negative regulation of translation GO:0017148 IEA
    protein maturation by protein folding GO:0022417 IC
    endoplasmic reticulum unfolded protein response GO:0030968 IBA
    pancreas development GO:0031016 IEA
    positive regulation of protein ubiquitination GO:0031398 IDA
    positive regulation of protein ubiquitination GO:0031398 ISS
    endoplasmic reticulum calcium ion homeostasis GO:0032469 IDA
    response to endoplasmic reticulum stress GO:0034976 IDA
    ERAD pathway GO:0036503 IDA
    ERAD pathway GO:0036503 ISS
    olfactory behavior GO:0042048 IEA
    glucose homeostasis GO:0042593 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of programmed cell death GO:0043069 IMP
    negative regulation of neuron apoptotic process GO:0043524 IMP
    positive regulation of growth GO:0045927 ISS
    protein stabilization GO:0050821 IDA
    protein stabilization GO:0050821 IMP
    protein stabilization GO:0050821 ISS
    protein stabilization GO:0050821 TAS
    nervous system process GO:0050877 IMP
    positive regulation of protein metabolic process GO:0051247 IDA
    positive regulation of calcium ion transport GO:0051928 IDA
    calcium ion homeostasis GO:0055074 IBA
    calcium ion homeostasis GO:0055074 IDA
    negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902236 IEA
    negative regulation of response to endoplasmic reticulum stress GO:1903573 IMP
    negative regulation of ATF6-mediated unfolded protein response GO:1903892 IDA
    positive regulation of ERAD pathway GO:1904294 IEA
    negative regulation of type B pancreatic cell apoptotic process GO:2000675 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    secretory granule GO:0030141 IDA
    dendrite GO:0030425 ISS
    synaptic vesicle membrane GO:0030672 IEA
 Experiment description of studies that identified WFS1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for WFS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STARD3NL 83930
Affinity Capture-MS Homo sapiens
2 F2RL1  
Two-hybrid Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 IMMT 10989
Co-fractionation Homo sapiens
5 SEC63 11231
Proximity Label-MS Homo sapiens
6 LYPD3 27076
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 VIPR2  
Affinity Capture-MS Homo sapiens
9 ADORA2B  
Affinity Capture-MS Homo sapiens
10 HSD3B7 80270
Proximity Label-MS Homo sapiens
11 TMEM9 252839
Affinity Capture-MS Homo sapiens
12 DHCR24 1718
Affinity Capture-MS Homo sapiens
13 CLGN 1047
Affinity Capture-MS Homo sapiens
14 KLK8 11202
Affinity Capture-MS Homo sapiens
15 GPR17 2840
Affinity Capture-MS Homo sapiens
16 RAB7A 7879
Proximity Label-MS Homo sapiens
17 TP53 7157
Affinity Capture-MS Homo sapiens
18 ATF6  
Affinity Capture-Western Homo sapiens
19 SYNGR1 9145
Affinity Capture-MS Homo sapiens
20 EIF6 3692
Two-hybrid Homo sapiens
21 PXMP2  
Proximity Label-MS Homo sapiens
22 ACKR2  
Affinity Capture-MS Homo sapiens
23 HSD17B11 51170
Proximity Label-MS Homo sapiens
24 TPTE  
Affinity Capture-MS Homo sapiens
25 UBE2L6 9246
Affinity Capture-MS Homo sapiens
26 OPALIN  
Affinity Capture-MS Homo sapiens
27 CD274 29126
Affinity Capture-MS Homo sapiens
28 EDEM1  
Affinity Capture-MS Homo sapiens
29 MGARP  
Affinity Capture-MS Homo sapiens
30 NDUFS4 4724
Co-fractionation Homo sapiens
31 TSPAN15 23555
Affinity Capture-MS Homo sapiens
32 SMURF1 57154
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 TYROBP  
Affinity Capture-MS Homo sapiens
34 GJD3  
Proximity Label-MS Homo sapiens
35 EMD 2010
Proximity Label-MS Homo sapiens
36 DNAJC25 548645
Proximity Label-MS Homo sapiens
37 CALM1 801
Affinity Capture-MS Homo sapiens
38 ANTXR1 84168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TBL2 26608
Co-fractionation Homo sapiens
40 CD79A  
Two-hybrid Homo sapiens
41 TMEM72  
Affinity Capture-MS Homo sapiens
42 SYVN1 84447
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
43 P2RY8  
Affinity Capture-MS Homo sapiens
44 FFAR1  
Affinity Capture-MS Homo sapiens
45 LTB4R2  
Two-hybrid Homo sapiens
46 FAM209A 200232
Two-hybrid Homo sapiens
47 PSMA5 5686
Affinity Capture-Western Homo sapiens
48 MAPK6  
Two-hybrid Homo sapiens
49 TERF1 7013
Two-hybrid Homo sapiens
50 NDUFA2 4695
Co-fractionation Homo sapiens
51 TUSC5  
Two-hybrid Homo sapiens
52 PBXIP1 57326
Affinity Capture-MS Homo sapiens
53 SEC62 7095
Proximity Label-MS Homo sapiens
54 ATP2A1 487
Proximity Label-MS Homo sapiens
55 FBXO2 26232
Affinity Capture-MS Homo sapiens
56 CMTM5  
Affinity Capture-MS Homo sapiens
57 EFNA4  
Affinity Capture-MS Homo sapiens
58 GPR182  
Affinity Capture-MS Homo sapiens
59 TNFRSF9  
Affinity Capture-MS Homo sapiens
60 TMEM231 79583
Affinity Capture-MS Homo sapiens
61 C5AR1  
Affinity Capture-MS Homo sapiens
62 NDUFS1 4719
Co-fractionation Homo sapiens
63 RPN1 6184
Proximity Label-MS Homo sapiens
64 DNAJC1 64215
Proximity Label-MS Homo sapiens
65 P2RX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 TMEM184A  
Affinity Capture-MS Homo sapiens
68 SSR1 6745
Proximity Label-MS Homo sapiens
69 CD3E 916
Affinity Capture-MS Homo sapiens
70 CHCHD3 54927
Co-fractionation Homo sapiens
71 GPR84  
Affinity Capture-MS Homo sapiens
72 CALM3 808
Affinity Capture-MS Homo sapiens
73 CANX 821
Affinity Capture-MS Homo sapiens
74 ADIPOR1 51094
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 CLEC4E  
Affinity Capture-MS Homo sapiens
76 PDIA3 2923
Cross-Linking-MS (XL-MS) Homo sapiens
77 GPR173  
Affinity Capture-MS Homo sapiens
78 ARMCX2 9823
Co-fractionation Homo sapiens
79 C3orf52  
Affinity Capture-MS Homo sapiens
80 NDUFB4 4710
Co-fractionation Homo sapiens
81 DRD2  
Two-hybrid Homo sapiens
82 CCDC47 57003
Affinity Capture-MS Homo sapiens
83 CYBA 1535
Co-fractionation Homo sapiens
View the network image/svg+xml



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