Gene description for NDUFB4
Gene name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
Gene symbol NDUFB4
Other names/aliases B15
CI-B15
Species Homo sapiens
 Database cross references - NDUFB4
ExoCarta ExoCarta_4710
Vesiclepedia VP_4710
Entrez Gene 4710
HGNC 7699
MIM 603840
UniProt O95168  
 NDUFB4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDUFB4
Molecular Function
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified NDUFB4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDUFB4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFV1 4723
Affinity Capture-MS Homo sapiens
2 PHB 5245
Co-fractionation Homo sapiens
3 PPP4R1 9989
Affinity Capture-MS Homo sapiens
4 BAG3 9531
Affinity Capture-MS Homo sapiens
5 NDUFA1 4694
Affinity Capture-MS Homo sapiens
6 Rab5c 19345
Affinity Capture-MS Mus musculus
7 C15orf48  
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 NDUFA11 126328
Affinity Capture-MS Homo sapiens
10 SPRTN  
Affinity Capture-MS Homo sapiens
11 Ercc6l  
Affinity Capture-MS Mus musculus
12 FTL 2512
Affinity Capture-MS Homo sapiens
13 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
15 VAPA 9218
Affinity Capture-MS Homo sapiens
16 FFAR1  
Affinity Capture-MS Homo sapiens
17 NDUFA9 4704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
19 MME 4311
Affinity Capture-MS Homo sapiens
20 AURKA 6790
Affinity Capture-MS Homo sapiens
21 M6PR 4074
Co-fractionation Homo sapiens
22 Bmpr1a  
Affinity Capture-MS Mus musculus
23 MPV17 4358
Co-fractionation Homo sapiens
24 Rmdn3  
Affinity Capture-MS Mus musculus
25 FOXA1  
Affinity Capture-MS Homo sapiens
26 NDUFS4 4724
Affinity Capture-MS Homo sapiens
27 TCF3  
Affinity Capture-MS Homo sapiens
28 NDUFA12 55967
Affinity Capture-MS Homo sapiens
29 Tmed2 56334
Affinity Capture-MS Mus musculus
30 NDUFA4 4697
Affinity Capture-MS Homo sapiens
31 NUP35 129401
Proximity Label-MS Homo sapiens
32 SNX27 81609
Affinity Capture-MS Homo sapiens
33 MARC1  
Co-fractionation Homo sapiens
34 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 UQCRFS1 7386
Co-fractionation Homo sapiens
36 Cdc16  
Affinity Capture-MS Mus musculus
37 ABCD1 215
Co-fractionation Homo sapiens
38 RPA3 6119
Proximity Label-MS Homo sapiens
39 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 NDUFB3 4709
Affinity Capture-MS Homo sapiens
41 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 RAB7A 7879
Affinity Capture-MS Homo sapiens
43 NDUFA7 4701
Affinity Capture-MS Homo sapiens
44 TIMMDC1  
Affinity Capture-MS Homo sapiens
45 RIPK4  
Affinity Capture-MS Homo sapiens
46 Kctd5  
Affinity Capture-MS Mus musculus
47 WFS1 7466
Co-fractionation Homo sapiens
48 OGDH 4967
Co-fractionation Homo sapiens
49 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
50 VDAC1 7416
Co-fractionation Homo sapiens
51 DNAJC30  
Affinity Capture-MS Homo sapiens
52 NDUFV2 4729
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
53 LMNA 4000
Proximity Label-MS Homo sapiens
54 SCARA3  
Affinity Capture-MS Homo sapiens
55 NDUFV3 4731
Affinity Capture-MS Homo sapiens
56 MYL12A 10627
Co-fractionation Homo sapiens
57 LAMTOR3 8649
Co-fractionation Homo sapiens
58 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 TOMM5  
Co-fractionation Homo sapiens
60 TOMM20 9804
Co-fractionation Homo sapiens
61 FUT1  
Affinity Capture-MS Homo sapiens
62 GOLT1B 51026
Affinity Capture-MS Homo sapiens
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