Gene description for SLC2A5
Gene name solute carrier family 2 (facilitated glucose/fructose transporter), member 5
Gene symbol SLC2A5
Other names/aliases GLUT-5
GLUT5
Species Homo sapiens
 Database cross references - SLC2A5
ExoCarta ExoCarta_6518
Vesiclepedia VP_6518
Entrez Gene 6518
HGNC 11010
MIM 138230
UniProt P22732  
 SLC2A5 identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for SLC2A5
Molecular Function
    fructose transmembrane transporter activity GO:0005353 IBA
    fructose transmembrane transporter activity GO:0005353 IDA
    fructose transmembrane transporter activity GO:0005353 TAS
    protein binding GO:0005515 IPI
    D-glucose transmembrane transporter activity GO:0055056 IBA
    D-glucose transmembrane transporter activity GO:0055056 IEA
    fructose binding GO:0070061 ISS
Biological Process
    regulation of systemic arterial blood pressure mediated by a chemical signal GO:0003044 ISS
    carbohydrate metabolic process GO:0005975 TAS
    response to fructose GO:0009750 ISS
    fructose transmembrane transport GO:0015755 IBA
    fructose transmembrane transport GO:0015755 IDA
    D-glucose import GO:0046323 IBA
    dehydroascorbic acid transport GO:0070837 IBA
    cellular response to fructose stimulus GO:0071332 ISS
    intestinal hexose absorption GO:0106001 TAS
    D-glucose transmembrane transport GO:1904659 IEA
    fructose import across plasma membrane GO:1990539 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    apical plasma membrane GO:0016324 ISS
    specific granule membrane GO:0035579 TAS
    sarcolemma GO:0042383 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SLC2A5 in exosomes
1
Experiment ID 13
MISEV standards
IEM
Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC2A5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Two-hybrid Homo sapiens
2 LMBRD2 92255
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ALDH1L1 10840
Affinity Capture-MS Homo sapiens
4 MTERF3  
Two-hybrid Homo sapiens
5 FITM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FCRL3  
Two-hybrid Homo sapiens
8 OSBPL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ATP12A 479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TCF7L2  
Co-localization Homo sapiens
11 CCL5 6352
Two-hybrid Homo sapiens
12 SLC7A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 EVI2B 2124
Two-hybrid Homo sapiens
14 TMEM237 65062
Two-hybrid Homo sapiens
15 LPGAT1 9926
Affinity Capture-MS Homo sapiens
16 FTO 79068
Affinity Capture-MS Homo sapiens
17 TMEM130  
Two-hybrid Homo sapiens
18 ZMPSTE24 10269
Affinity Capture-MS Homo sapiens
19 KIAA0368 23392
Affinity Capture-MS Homo sapiens
20 ATP6V0B 533
Two-hybrid Homo sapiens
21 ARL13B 200894
Two-hybrid Homo sapiens
22 RBFOX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 SCN3B  
Two-hybrid Homo sapiens
24 MGST3 4259
Two-hybrid Homo sapiens
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