Gene description for ATP6V0B
Gene name ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b
Gene symbol ATP6V0B
Other names/aliases ATP6F
HATPL
VMA16
Species Homo sapiens
 Database cross references - ATP6V0B
ExoCarta ExoCarta_533
Entrez Gene 533
HGNC 861
MIM 603717
UniProt Q99437  
 ATP6V0B identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for ATP6V0B
Molecular Function
    transporter activity GO:0005215 TAS
    hydrogen ion transmembrane transporter activity GO:0015078 IEA
Biological Process
    transferrin transport GO:0033572 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    phagosome maturation GO:0090382 TAS
    proton transport GO:0015992 TAS
    ATP hydrolysis coupled proton transport GO:0015991 IEA
    cellular iron ion homeostasis GO:0006879 TAS
    ion transmembrane transport GO:0034220 TAS
    transmembrane transport GO:0055085 TAS
    interaction with host GO:0051701 TAS
Subcellular Localization
    vacuolar membrane GO:0005774 IEA
    integral component of membrane GO:0016021 IEA
    phagocytic vesicle membrane GO:0030670 TAS
    proton-transporting V-type ATPase, V0 domain GO:0033179 IEA
    endosome membrane GO:0010008 TAS
 Experiment description of studies that identified ATP6V0B in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ATP6V0B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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