Gene description for ATP6V0B
Gene name ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b
Gene symbol ATP6V0B
Other names/aliases ATP6F
HATPL
VMA16
Species Homo sapiens
 Database cross references - ATP6V0B
ExoCarta ExoCarta_533
Vesiclepedia VP_533
Entrez Gene 533
HGNC 861
MIM 603717
UniProt Q99437  
 ATP6V0B identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for ATP6V0B
Molecular Function
    protein binding GO:0005515 IPI
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
Biological Process
    vacuolar acidification GO:0007035 NAS
    lysosomal lumen acidification GO:0007042 NAS
    regulation of macroautophagy GO:0016241 NAS
    endosomal lumen acidification GO:0048388 NAS
    intracellular pH reduction GO:0051452 NAS
    Golgi lumen acidification GO:0061795 NAS
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    vacuolar proton-transporting V-type ATPase, V0 domain GO:0000220 ISS
    lysosomal membrane GO:0005765 NAS
    lysosomal membrane GO:0005765 TAS
    plasma membrane GO:0005886 NAS
    endosome membrane GO:0010008 NAS
    endosome membrane GO:0010008 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    clathrin-coated vesicle membrane GO:0030665 IEA
    phagocytic vesicle membrane GO:0030670 TAS
    proton-transporting V-type ATPase complex GO:0033176 NAS
 Experiment description of studies that identified ATP6V0B in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ATP6V0B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 MS4A3  
Two-hybrid Homo sapiens
3 GJA8  
Two-hybrid Homo sapiens
4 MIP  
Two-hybrid Homo sapiens
5 FAS 355
Two-hybrid Homo sapiens
6 INSIG2  
Two-hybrid Homo sapiens
7 EMP1  
Two-hybrid Homo sapiens
8 TM4SF19  
Two-hybrid Homo sapiens
9 YWHAE 7531
Reconstituted Complex Homo sapiens
10 ADRB2  
Two-hybrid Homo sapiens
11 GLP1R  
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
12 GPR97  
Two-hybrid Homo sapiens
13 MTNR1B  
Two-hybrid Homo sapiens
14 NRM 11270
Two-hybrid Homo sapiens
15 STOM 2040
Two-hybrid Homo sapiens
16 DDX58 23586
Affinity Capture-RNA Homo sapiens
17 TMEM242  
Two-hybrid Homo sapiens
18 SPACA1 81833
Two-hybrid Homo sapiens
19 NXF1 10482
Affinity Capture-RNA Homo sapiens
20 MFF 56947
Two-hybrid Homo sapiens
21 APOD 347
Two-hybrid Homo sapiens
22 GPR42  
Two-hybrid Homo sapiens
23 MOV10 4343
Affinity Capture-RNA Homo sapiens
24 SREBF2 6721
Negative Genetic Homo sapiens
25 COL4A5  
Two-hybrid Homo sapiens
26 SLC10A1  
Two-hybrid Homo sapiens
27 SLC2A5 6518
Two-hybrid Homo sapiens
28 GPR61  
Two-hybrid Homo sapiens
29 KRAS 3845
Negative Genetic Homo sapiens
30 VMA21  
Two-hybrid Homo sapiens
31 KLRC1  
Two-hybrid Homo sapiens
32 YWHAG 7532
Two-hybrid Homo sapiens
33 IRAK3 11213
Two-hybrid Homo sapiens
34 FAM210B  
Two-hybrid Homo sapiens
View the network image/svg+xml



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