Gene description for MSRB3
Gene name methionine sulfoxide reductase B3
Gene symbol MSRB3
Other names/aliases DFNB74
Species Homo sapiens
 Database cross references - MSRB3
ExoCarta ExoCarta_253827
Vesiclepedia VP_253827
Entrez Gene 253827
HGNC 27375
MIM 613719
UniProt Q8IXL7  
 MSRB3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MSRB3
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    peptide-methionine (R)-S-oxide reductase activity GO:0033743 IBA
    peptide-methionine (R)-S-oxide reductase activity GO:0033743 IDA
    peptide-methionine (R)-S-oxide reductase activity GO:0033743 TAS
    L-methionine-(R)-S-oxide reductase activity GO:0033745 IEA
Biological Process
    response to oxidative stress GO:0006979 IEA
    protein repair GO:0030091 IDA
    protein repair GO:0030091 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IDA
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MSRB3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MSRB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TXN2 25828
Co-fractionation Homo sapiens
2 GLRX5 51218
Co-fractionation Homo sapiens
3 TRIP6 7205
Two-hybrid Homo sapiens
4 CHMP4C 92421
Affinity Capture-MS Homo sapiens
5 GABARAPL2 11345
Co-fractionation Homo sapiens
6 SPOP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PSAP 5660
Co-fractionation Homo sapiens
8 GUK1 2987
Co-fractionation Homo sapiens
9 AK2 204
Co-fractionation Homo sapiens
10 AK4 205
Co-fractionation Homo sapiens
11 KIF14 9928
Affinity Capture-MS Homo sapiens
12 LNX2  
Two-hybrid Homo sapiens
13 MTPN 136319
Co-fractionation Homo sapiens
14 BTBD9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EIF6 3692
Two-hybrid Homo sapiens
16 CTBP2 1488
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
17 UFSP1  
Two-hybrid Homo sapiens
18 ISCA2 122961
Co-fractionation Homo sapiens
19 REL 5966
Two-hybrid Homo sapiens
20 BANP  
Two-hybrid Homo sapiens
21 TCOF1 6949
Co-fractionation Homo sapiens
22 KRT34  
Two-hybrid Homo sapiens
23 RBP4 5950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SNX21  
Affinity Capture-MS Homo sapiens
25 LYRM2  
Co-fractionation Homo sapiens
26 PRDX5 25824
Co-fractionation Homo sapiens
27 KIF20A 10112
Affinity Capture-MS Homo sapiens
28 FHL3 2275
Two-hybrid Homo sapiens
29 KRT40  
Two-hybrid Homo sapiens
30 TRIM55  
Two-hybrid Homo sapiens
31 TXNDC12 51060
Co-fractionation Homo sapiens
32 NUDT19 390916
Co-fractionation Homo sapiens
33 METTL21B  
Two-hybrid Homo sapiens
34 FTH1 2495
Affinity Capture-MS Homo sapiens
35 AZIN1 51582
Affinity Capture-MS Homo sapiens
36 WDR61 80349
Two-hybrid Homo sapiens
37 NXF1 10482
Affinity Capture-RNA Homo sapiens
38 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 AURKB 9212
Affinity Capture-MS Homo sapiens
40 TXN 7295
Co-fractionation Homo sapiens
41 MED12L  
Two-hybrid Homo sapiens
42 PLEKHG4  
Two-hybrid Homo sapiens
43 POU6F2  
Two-hybrid Homo sapiens
44 TCF4  
Two-hybrid Homo sapiens
45 TIGAR 57103
Co-fractionation Homo sapiens
46 GAPDH 2597
Co-fractionation Homo sapiens
47 CHMP4B 128866
Affinity Capture-MS Homo sapiens
48 C1QL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 TRIM23 373
Two-hybrid Homo sapiens
50 ASB13  
Two-hybrid Homo sapiens
51 AK3 50808
Co-fractionation Homo sapiens
52 PTRHD1 391356
Co-fractionation Homo sapiens
53 METTL21A  
Two-hybrid Homo sapiens
54 STIM2 57620
Affinity Capture-MS Homo sapiens
55 MOV10 4343
Affinity Capture-RNA Homo sapiens
56 PRC1 9055
Affinity Capture-MS Homo sapiens
57 DCUN1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 COA7  
Co-fractionation Homo sapiens
59 KLHL7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 LCN2 3934
Two-hybrid Homo sapiens
61 TBCA 6902
Co-fractionation Homo sapiens
62 FXN  
Co-fractionation Homo sapiens
63 PRDM4  
Two-hybrid Homo sapiens
64 METTL15  
Two-hybrid Homo sapiens
65 IFNA21  
Affinity Capture-MS Homo sapiens
66 FAM168B 130074
Two-hybrid Homo sapiens
67 EFEMP1 2202
Two-hybrid Homo sapiens
68 TRIM63  
Two-hybrid Homo sapiens
69 SRI 6717
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which MSRB3 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Protein repair TAS Reactome





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